Matrix metalloproteinases regulate extracellular levels of SDF-1/CXCL12, IL-6 and VEGF in hydrogen peroxide-stimulated human periodontal ligament fibroblasts

Cavalla, Franco; Osorio, C; Paredes, Rodolfo; Valenzuela, Manuel; García-Sesnich, Jocelyn; Sorsa, Timo; Tervahartiala, Taina; Hernández, Marcela

doi:10.1016/j.cyto.2015.02.001

Cited by 31 publications

(30 citation statements)

References 49 publications

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“…Cavalla et al reported that IL-6 and VEGF expressions of human periodontal ligament fibroblast in oxidative stress model were higher than in non-oxidative stress model (41). Matsui et al reported that reactive oxygen species inhibited production of type I collagen mRNA and collagen protein in 24-and 72-h HGF cultures (42).…”

Section: Discussionmentioning

confidence: 99%

Effects of cleaning methods for custom abutment surfaces on gene expression of human gingival fibroblasts

et al. 2017

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The aim of this study was to develop an effective method for cleaning implant abutments made by computer-aided design and computer-aided manufacturing techniques and to investigate the effect of decontamination in vitro. Briefly, a newly developed reagent (PK) and/or vacuum plasma (Plasma) were used to clean the surfaces of zirconia disks, and the effects of this decontamination were evaluated by X-ray photoelectron spectroscopy. Human gingival fibroblasts (HGFs) were cultured on sample disks for 6, 24, and 48 h. We evaluated cell attachment and gene expression of the acute inflammatory cytokines interleukin-6 and vascular endothelial growth factor A, and type 1 collagen. In the PK and PK+Plasma groups, surface contaminants were reduced by washing. In addition, HGF attachments was increased in the PK and PK+Plasma groups. Gene expressions of interleukin-6 and vascular endothelial growth factor A were lower at 6 h. Gene expression of type 1 collagen was increased at all time points after seeding. These results suggest that decontamination of implant abutment surfaces is important in initial HGF attachment and may improve the biological seal of peri-implant soft tissue.

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Section: Discussionmentioning

confidence: 99%

Effects of cleaning methods for custom abutment surfaces on gene expression of human gingival fibroblasts

et al. 2017

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“…In a recent article, we demonstrated that MMP activity decreased the secreted levels of IL-6, while at the same time it was responsible for increasing the secreted levels of CXC motif chemokine ligand 12 (CXCL12) and vascular endothelial growth factor A (VEGF A), and reduced cell migration in an in vitro system of human periodontal ligament fibroblasts [63]. The target-specific effect of MMP activity highlights the complexity of the networks that are at play during the inflammatory process.…”

Section: Mmp-mediated Regulation Of Inflammatory Responsementioning

confidence: 99%

Matrix Metalloproteinases as Regulators of Periodontal Inflammation

Cavalla

Patricia

Sorsa

et al. 2017

IJMS

232

196

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Periodontitis are infectious diseases characterized by immune-mediated destruction of periodontal supporting tissues and tooth loss. Matrix metalloproteinases (MMPs) are key proteases involved in destructive periodontal diseases. The study and interest in MMP has been fuelled by emerging evidence demonstrating the broad spectrum of molecules that can be cleaved by them and the myriad of biological processes that they can potentially regulate. The huge complexity of MMP functions within the ‘protease web’ is crucial for many physiologic and pathologic processes, including immunity, inflammation, bone resorption, and wound healing. Evidence points out that MMPs assemble in activation cascades and besides their classical extracellular matrix substrates, they cleave several signalling molecules—such as cytokines, chemokines, and growth factors, among others—regulating their biological functions and/or bioavailability during periodontal diseases. In this review, we provide an overview of emerging evidence of MMPs as regulators of periodontal inflammation.

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“…The extraction and isolation of HPLF from the periodontal ligaments were performed as previously described (Cavalla et al . , Osorio et al . ) and incubated in a humidified atmosphere with 5% CO 2 at 37 °C in DMEM supplemented with 100 IU mL −1 penicillin, 100 mg mL −1 streptomycin and 10% FBS.…”

Section: Methodsmentioning

confidence: 99%

“…, Cavalla et al . ). To further understand the role of HPLF in local and systemic events associated with apical periodontitis, the aim of this study was to characterize the potential of human PDL fibroblasts to synthesize CRP and Th‐related cytokines in response to IL‐6 in periodontal health and apical inflammation.…”

Section: Introductionmentioning

confidence: 97%

Human periodontal ligament fibroblasts synthesize C‐reactive protein and Th‐related cytokines in response to interleukin (IL)‐6 trans‐signalling

et al. 2017

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Matrix metalloproteinases regulate extracellular levels of SDF-1/CXCL12, IL-6 and VEGF in hydrogen peroxide-stimulated human periodontal ligament fibroblasts

Cited by 31 publications

References 49 publications

Effects of cleaning methods for custom abutment surfaces on gene expression of human gingival fibroblasts

Effects of cleaning methods for custom abutment surfaces on gene expression of human gingival fibroblasts

Matrix Metalloproteinases as Regulators of Periodontal Inflammation

Human periodontal ligament fibroblasts synthesize C‐reactive protein and Th‐related cytokines in response to interleukin (IL)‐6 trans‐signalling

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