Matrix metalloproteinases in choriocarcinoma cell lines: A potential regulatory role of extracellular matrix components

Maquoi, Erik; Noël, Agnès; Foidart, Jean‐Michel

doi:10.1016/s0143-4004(97)80084-7

Cited by 3 publications

(2 citation statements)

References 57 publications

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“…Preliminary biocompatibility experiments have shown a slight decrease in viable cell count when cells were plated on GelMOD-AEMA only, but this drawback was successfully tackled by using fibronectin coatings. The improved proliferation of both cells can be explained by the fact that the extra cellular matrix (ECM) proteins gelatin and fibronectin promote cell adhesion and spreading[ 29 ]. Several studies have shown that fibronectin plays a predominant role in adhesion processes, to neighboring cells as well as to substrate material, in various cell types[ 30 – 33 ].…”

Section: Discussionmentioning

confidence: 99%

Fabrication of biomimetic placental barrier structures within a microfluidic device utilizing two-photon polymerization

Mandt¹,

Gruber²,

Markovic³

et al. 2024

IJB

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The placenta is a transient organ, essential for development and survival of the unborn fetus. It interfaces the body of the pregnant woman with the unborn child and secures transport of endogenous and exogenous substances. Maternal and fetal blood are thereby separated at any time, by the so-called placental barrier. Current in vitro approaches fail to model this multifaceted structure, therefore research in the field of placental biology is particularly challenging. The present study aimed at establishing a novel model, simulating placental transport and its implications on development, in a versatile but reproducible way. The basal membrane was replicated using a gelatin-based material, closely mimicking the composition and properties of the natural extracellular matrix. The microstructure was produced by using a high-resolution 3D printing method – the two-photon polymerization (2PP). In order to structure gelatin by 2PP, its primary amines and carboxylic acids are modified with methacrylamides and methacrylates (GelMOD-AEMA), respectively. High-resolution structures in the range of a few micrometers were produced within the intersection of a customized microfluidic device, separating the x-shaped chamber into two isolated cell culture compartments. Human umbilical-vein endothelial cells (HUVEC) seeded on one side of this membrane simulate the fetal compartment while human choriocarcinoma cells, isolated from placental tissue (BeWo B30) mimic the maternal syncytium. This barrier model in combination with native flow profiles can be used to mimic the microenvironment of the placenta, investigating different pharmaceutical, clinical and biological scenarios. As proof-of-principle, this bioengineered placental barrier was used for the investigation of transcellular transport processes. While high molecular weight substances did not permeate, smaller molecules in the size of glucose were able to diffuse through the barrier in a time-depended manner. We envision to apply this bioengineered placental barrier for pathophysiological research, where altered nutrient transport is associated with health risks for the fetus.

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Section: Discussionmentioning

confidence: 99%

Fabrication of biomimetic placental barrier structures within a microfluidic device utilizing two-photon polymerization

Mandt¹,

Gruber²,

Markovic³

et al. 2024

IJB

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“…Differences in the reported findings concerning MMP-9 could be due to cell culture conditions. According to Maquoi et al [1997] both the MMP-2 and MMP-9 are expressed in choriocarcinoma cells only when cells are cultured on or in ECM substrates [as by Librach et al, 1991] whereas MMP-9 is not produced on tissue culture plastic (culture system used in our investigations on the proteinase production and by Graham et al [1994]). The MMP-9 gene is known to be downregulated by progesterone and, therefore, reduced invasiveness and reduced expression of MMP-9 are in line with higher levels of progesterone in term placenta [Shimonovitz et al, 1998;Bischof et al, 2001].…”

Section: Modulation Of Matrix Interactions As Related To Invasionmentioning

confidence: 99%

Experimental Modulation of Cell-Cell Adhesion, Invasiveness and Differentiation in Trophoblast Cells

Hohn¹,

Denker²

2002

Cells Tissues Organs

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The establishment of pregnancy in the human decisively depends on the competence of the early trophoblast to interact during implantation with (1) the uterine epithelium and subsequently (2) with the endometrial stroma and blood vessels. In the interaction with uterine epithelium cell-to-cell adhesion appears to be a critical element, involving initially (and astonishingly) apical cell poles of both epithelia. The subsequent invasion of the stroma includes both adhesive interactions with and degradation of extracellular matrix. How these different processes are regulated in detail remains largely unknown. While the invasiveness of the trophoblast is known to be regulated in local and temporal terms it has remained unclear so far whether trophoblast adhesiveness to cells and/or matrix is subject to a coupled regulation or whether both properties involve different, maybe sequentially effective, control mechanisms. It is also not known how the regulation of these activities is related to the differentiation pathways leading to the formation of noninvasive villous trophoblast serving endocrine as well as nutritive functions. This communication reviews experiments using normal cytotrophoblast cells isolated from first trimester or term placentae as well as malignant trophoblast (choriocarcinoma) cells treated with a panel of compounds known to modulate cell differentiation [retinoic acid, methotrexate, dibutyryl-cAMP, phorbol-(12-myristoyl-13-acetyl)-diester]. Parameters indicative of trophoblast differentiation [in particular chorionic gonadotrophin (hCG) secretion] as well as adhesion to uterine epithelial cells and invasion into extracellular matrix in vitro were monitored. While expression of differentiation parameters was increased by all drug treatments, adhesion to uterine epithelial cells in vitro was reduced. Modulation of invasiveness, however, followed a different pattern: while it was reduced in normal trophoblast cells it was even increased in choriocarcinoma cells with various substances. The response of cells with respect to production of extracellular matrix proteins or matrix-degrading proteinases showed a complex pattern that again lacked a stringent correlation with hCG production and adhesion, and in addition also with invasive behavior. These results suggest that adhesiveness of trophoblast to uterine epithelial cells and invasiveness into the uterine stroma (extracellular matrix) are subject to different control mechanisms. They support the view that trophoblast-endometrium interactions involve a cascade of various adhesion and migration processes whose cellular and molecular basis is complex but accessible to experimental investigation using a variety of available in vitro systems.

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Biological Function of Hyperglycosylated hCG

Cole

2010

Human Chorionic Gonadotropin

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Matrix metalloproteinases in choriocarcinoma cell lines: A potential regulatory role of extracellular matrix components

Cited by 3 publications

References 57 publications

Fabrication of biomimetic placental barrier structures within a microfluidic device utilizing two-photon polymerization

Fabrication of biomimetic placental barrier structures within a microfluidic device utilizing two-photon polymerization

Experimental Modulation of Cell-Cell Adhesion, Invasiveness and Differentiation in Trophoblast Cells

Biological Function of Hyperglycosylated hCG

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