Matrix metalloproteinases and their inhibitors in gingival crevicular fluid and saliva of periodontitis patients

Abstract

Matrix metalloproteinases (MMPs) and serine proteinases seem to be related to tissue destruction in periodontitis. The presence of MMPs in gingival crevicular fluid (GCF) and saliva, however, has not been studied comprehensively with the enzyme-linked immunosorbent assay (ELISA)-technique. We therefore examined the levels of MMP-1, -3, -8 and -9, and their endogenous inhibitor, tissue inhibitor of matrix metalloproteinases (TIMP-1), in GCF and saliva of patients with adult periodontitis (AP) and localized juvenile periodontitis (LJP). Elevated levels of MMP-1 were detected in LJP GCF compared to AP and control GCF. Elevated levels of TIMP-1 were also detected in LJP GCF in comparison to AP and control GCF. Higher MMP-8 levels were detected in AP GCF compared to LJP and control GCF. The relative low levels of MMP-3 were present in all studied GCF samples. Elevated levels of MMP-8 were further detected in saliva of AP compared to LJP and the controls. Both MMP-1 and TIMP-1 were detected in all studied saliva samples, but not significant differences were detected between the studied groups. Our ELISA-results confirm that (i) PMN MMP-8 and MMP-9 are the main collagenase and gelatinase in AP GCF, whereas GCF collagenase in LJP seems to be of the MMP-1-type; (ii) only low levels of TIMP-1, endogenous MMP-inhibitor, are present in AP GCF, which emphasises the importance of doxycycline as a possible adjunctive drug in the treatment of AP patients; (iii) tests based on specific antibodies against PMN MMPs, especially MMP-8, might serve as a reliable method of measuring and monitoring enzyme levels in GCF from different periodontitis patients.

Bioactive Chitosan Nanoparticles and Photodynamic Therapy Inhibit Collagen Degradation In Vitro

“…The residual bacteria may activate dentinal MMPs, contributing to the breakdown of demineralized collagen matrices and weakened dentin structure. MMPs, as collagenase and gelatinase have been found in gingival crevicular fluid and saliva (55)(56)(57)(58). Given the various sources of collagenase, a non-specific collagenase inhibitor with high inhibition, physico-chemical characteristic to adhere to inherently moistened dentin, and with substantivity is desired.…”

The Role of Chemokines and Cytokines in the Pathogenesis of Periodontal and Periapical Lesions: Current Concepts

“…It is thought that MMPs and TIMPs are involved in the physiological turnover of periodontal tissues, and MMPs appear to be involved in tissue destruction in PDs (Birkedal-Hansen, 1993, Golub et al, 2001, Reynolds et al, 1994, Van der Zee et al, 1997. However, there are contradictory results regarding the balance of MMPs/TIMPs in pathological versus healthy gingival samples (Aiba et al, 1996, Dahan et al, 2001, Garlet et al, 2004, Ingman et al, 1996, Kubota et al, 1996, Nomura et al, 1998. Some studies show a decrease in the levels of TIMPs in diseased periodontal tissues, supporting the idea that an imbalance in the levels of TIMPs/MMPs occurs in PDs and results in tissue destruction , Soell et al, 2002, Tuter et al, 2002.…”

Matrix metalloproteinase-8 and substance P levels in root canal exudates of nonvital teeth

“…2,[11][12][13][14][15][16] A time resolved immunofluorometric assay (IFMA), ELISA 그리고 a dentoAnalyzer device 등을 이용해서 MMP-8을 분석해 본 결과 방법에 상관없이 일치하는 결과 를 보였다는 연구도 있었지만, 16 ELISA의 경우 다른 방법 들과는 다른 종류의 antibody를 사용하게 되어서 조금 다 른 결과를 보일 수도 있다고 보고되기도 했다. 17 치주영역에 서는 치은 열구액을 채취하여 chair-side에서 MMP-8의 농도를 측정하여 진단에 이용할 수 있는 기구가 개발되어 있다.…”