Matrix metalloproteinases and their inhibitors in gastric cancer

Murray, Graeme I.; Duncan, Margaret E.; Arbuckle, Erin; Melvin, William T.; Fothergill, John E.

doi:10.1136/gut.43.6.791

Cited by 145 publications

(123 citation statements)

References 31 publications

Supporting

Mentioning

112

Contrasting

Unclassified

Order By: Relevance

“…TIMP-1 is a ubiquitous glycoprotein capable of inhibiting all activated collagenases (Gomez et al, 1997). Tissue inhibitor of metalloproteinases were previously found not to be correlated with tumour stage, histological type, lymph node status or survival in human gastric cancer (Murray et al, 1998). We did not find any relation of TIMP-1 372C4T with gastric carcinoma, protein level or survival of the patients.…”

Section: Discussioncontrasting

confidence: 77%

Clinical impact of MMP and TIMP gene polymorphisms in gastric cancer

et al. 2006

View full text Add to dashboard Cite

Gastric cancers express enhanced levels of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs). Single-nucleotide polymorphisms (SNPs) in MMP and TIMP genes may be associated with disease susceptibility and might also affect their antigen expression. We studied the genotype distribution and allele frequencies of SNPs of MMP-2, -7, -8 and -9 and TIMP-1 and -2 in gastric cancer patients in relation to tumour progression, patient survival and tissue antigen expression. The genotype distribution and allele frequencies were similar in gastric cancer patients and controls, except for MMP-7 À181A4G . In addition, the genotype distribution of MMP-7 À181A4G was associated with Helicobacter pylori status (w 2 7.8, P ¼ 0.005) and tumour-related survival of the patients. Single-nucleotide polymorphism TIMP-2 303C4T correlated significantly with the WHO classification (w 2 5.9, P ¼ 0.03) and also strongly with tumour-related survival (log rank 11.74, P ¼ 0.0006). Single-nucleotide polymorphisms of MMP-2, -8, -9 and TIMP-1 were not associated with tumour-related survival. Only the gene promoter MMP-2 À1306C4T polymorphism correlated significantly with the protein level within the tumours. First-order dendrogram cluster analysis combined with Cox analysis identified the MMP-7 À181A4G and TIMP-2 303C4T polymorphism combination to have a major impact on patients survival outcome. We conclude that MMP-related SNPs, especially MMP-7 À181A4G and TIMP-2 303C4T , may be helpful in identifying gastric cancer patients with a poor clinical outcome.

show abstract

Section: Discussioncontrasting

confidence: 77%

Clinical impact of MMP and TIMP gene polymorphisms in gastric cancer

et al. 2006

View full text Add to dashboard Cite

show abstract

“…We observed that co-culture with H. pylori stimulated AGS cell secretion of MMP-1, an important MMP identified within ulcer lesions (28,70). Because type I and III collagens predominate in gastric mucosa (29,44), and both are susceptible to MMP-1, (45), these data suggest a mechanism for H. pyloriinduced gastric stromal destruction.…”

Section: Discussionmentioning

confidence: 79%

Helicobacter pylori Stimulates Gastric Epithelial Cell MMP-1 Secretion via CagA-dependent and -independent ERK Activation

Pillinger

Marjanović

Kim

et al. 2007

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…Murray et al demonstrated that MMP-1 protein immunolocalized in carcinoma cells correlated with a poor prognosis in colorectal (Murray et al, 1996) and oesophageal carcinoma (Murray et al, 1998b), but not in gastric carcinoma (Murray et al, 1998a). However, in oesophageal carcinoma, the significance was minute regarding the prognosis and this report included 19 squamous cell carcinomas which showed only 3 cases (16%) of positive immunostaining of MMP-1 (Murray et al, 1998b).…”

Section: Discussionmentioning

confidence: 89%

The clinical significance of MMP-1 expression in oesophageal carcinoma

et al. 2001

View full text Add to dashboard Cite

Summary Matrix metalloproteinase-1 (MMP-1) is involved in the degradation of interstitial collagen and thus thought to play a role in invasion of carcinoma. We investigated 51 oesophageal carcinoma patients to clarify the significance of MMP-1. MMP-1 mRNA was demonstrated to be expressed exclusively in almost all carcinoma tissue specimens (T) (94.1%) by reverse transcription-polymerase chain reaction, but not found in normal mucosal tissue specimens (N). The mean T/N ratio of MMP-1 was 42.5 and cases with T/N ≥ 10 had a higher incidence of cases involving muscularis propria than those with T/N < 10 which included all the cases involving the submucosa (P < 0.05). MMP-1 mRNA was significantly associated with both 40 kD (putative active MMP-1) and 50 kD (putative latent MMP-1) gelatinolytic bands (n = 17). These findings indicated that MMP-1 mRNA reflected the net function of MMP-1 and suggested MMP-1 to be involved in carcinoma invasive process. On the other hand, MMP-1 mRNA was inversely correlated with the patient prognosis (P < 0.01). These results indicated that MMP-1 might therefore play a crucial role in local invasion, but not in systemic dissemination. As a result, MMP-1 might be a novel prognostic factor independent from those previously reported in oesophageal carcinoma. 276-282 © 2001 Cancer Research Campaign doi: 10.1054/ bjoc.2000.1568, available online at http://www.idealibrary.com on http://www.bjcancer.com basal cell carcinoma and one adenocarcinoma. The depth of invasion of the tumour was as follows, 5 involved the submucosa, 7 involved the muscularis propria, and 39 involved the adventitia or more. The cases with lymph node metastases were classified into two groups: the non-metastatic group (n = 8) and metastatic group (n = 43). Carcinoma specimens were obtained from the tumour edge, avoiding a necrotic centre and corresponding distant normal mucosa specimens were also obtained at least 5 cm away from the tumour edge by sharply dissecting the mucosa off the muscularis propria. All specimens were quick-frozen in liquid nitrogen and stored at Ϫ80˚C until processing.When obtaining the tumour tissue from the surgical specimens, we have carefully chosen the area where abundant tumour cells seemed to be contained. The cut surface of the tumour centre indicate the whitish area where usually contain a lot of tumour cells. Moreover we reviewed the specimens histologically, and almost all specimens (47/51) were ascertained that more than 80% area contained tumour cells and the remaining 4 showed that tumour cells occupied more than 70%. Thus we think the used specimens were good for the molecular analysis. RNA extraction, reverse transcription-polymerase chain reaction (RT-PCR) and Northern blot hybridizationTotal RNA isolation Frozen-tissue specimens were homogenized in guanidium thiocyanate and total RNAs were obtained by ultracentrifugation through a cesium chloride cushion as described previously (Mori et al, 1993;Yamashita et al, 2000).cDNA preparation and RT-PCR Eight µg of each total RNA was re...

show abstract

Matrix metalloproteinases and their inhibitors in gastric cancer

Cited by 145 publications

References 31 publications

Clinical impact of MMP and TIMP gene polymorphisms in gastric cancer

Clinical impact of MMP and TIMP gene polymorphisms in gastric cancer

Helicobacter pylori Stimulates Gastric Epithelial Cell MMP-1 Secretion via CagA-dependent and -independent ERK Activation

The clinical significance of MMP-1 expression in oesophageal carcinoma

Contact Info

Product

Resources

About