Matrix metalloproteinases 2 and 9 expression in canine normal prostate and with proliferative disorders

Faleiro, Mariana Batista Rodrigues; Croce, G; Toledo, Denise Caroline; Rodrigues, Marcela Marcondes Pinto; Batista, Aline Carvalho; Damasceno, Adilson Donizeti; Brito, Luiz Augusto Batista; Amorim, Renée Laufer; Moura, Veridiana Maria Brianezi Dignani de

doi:10.1590/s0103-84782013005000050

Cited by 9 publications

(10 citation statements)

References 36 publications

(37 reference statements)

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“…In canine PC, MMP-2 and MMP-9 were previously evaluated and the authors found overexpression when compared PC to normal prostate. However, the authors did not compare the results with collagen expression [40], as well as we did not measure MMPs in the present study. Based on this previous description in canine PC [39], MMP-2 and 9 overexpression can induce collagen degradation interfering in the collagen evaluation by IHC and PSR.…”

Section: Discussionmentioning

confidence: 85%

Characterization of Collagen Fibers (I, III, IV) and Elastin of Normal and Neoplastic Canine Prostatic Tissues

Calderón

Kobayashi

Vasconcelos

et al. 2019

Veterinary Sciences

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This study aimed to investigate collagen (Coll-I, III, IV) and elastin in canine normal prostate and prostate cancer (PC) using Picrosirius red (PSR) and Immunohistochemical (IHC) analysis. Eight normal prostates and 10 PC from formalin-fixed, paraffin-embedded samples were used. Collagen fibers area was analyzed with ImageJ software. The distribution of Coll-I and Coll-III was approximately 80% around prostatic ducts and acini, 15% among smooth muscle, and 5% surrounding blood vessels, in both normal prostate and PC. There was a higher median area of Coll-III in PC when compared to normal prostatic tissue (p = 0.001 for PSR and p = 0.05 for IHC). Immunostaining for Coll-IV was observed in the basal membrane of prostate acini, smooth muscle, blood vessels, and nerve fibers of normal and PC samples. Although there was no difference in Coll-IV area between normal tissue and PC, tumors with Gleason score 10 showed absence of Coll-IV, when compared to scores 6 and 8 (p = 0.0095). Elastic fibers were found in the septa dividing the lobules and around the prostatic acini of normal samples and were statistically higher in PC compared to normal tissue (p = 0.00229). Investigation of ECM components brings new information and should be correlated with prognosis in future studies.

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Section: Discussionmentioning

confidence: 85%

Characterization of Collagen Fibers (I, III, IV) and Elastin of Normal and Neoplastic Canine Prostatic Tissues

Calderón

Kobayashi

Vasconcelos

et al. 2019

Veterinary Sciences

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“…These authors suggest that collagen reduction in PC could be the result from high metalloproteinase activity. In a study from Faleiro et al [26] in canine PC, MMP-2 and MMP-9 were overexpressed, when compared to normal prostate, but the authors did not compare the results with collagen expression.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Collagens Fibers (I, III, IV) and Elastin in the Extracellular Matrix of Normal and Neoplastic Canine Prostate

Calderón¹,

Kobayashi²,

Vasconcelos³

et al. 2019

Preprint

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Collagen (Coll) is the most common protein in the extracellular matrix, responsible for providing tissue structure and support. In some types of cancer, including prostate cancer (PC) abundant collagen was identified and related to tumor progression and metastasis. This study aimed to investigate Coll-I, III, IV and elastin in normal canine prostatic tissue and PC, using the Picrosirius red (PSR) and Immunohistochemical (IHC) analysis. Eight normal prostates and 10 PC from formalin-fixed, paraffin-embedded samples were used. Collagen fibers area was analyzed with ImageJ software. The distribution of Coll-I and Col-III was approximately 80% around prostatic ducts and acini, 15% among smooth muscle and 5% around in blood vessels, in both normal prostate and PC. Immunostaining for type IV collagen was observed in the basal membrane of prostate acini, smooth muscle, blood vessels, and never fibers of normal and PC samples. Elastic fibers were found in the septa dividing the lobules and around the prostatic acini of normal samples. A high amount of elastic fibers was observed around the ducts and the urethra in normal and canine PC. The distribution and area percentage of staining for collagen are similar in normal and neoplastic canine prostate when analyzed with PSR and IHC.

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“…Immunohistochemistry was performed using a procedure modified from a previously described protocol using an automated staining system (BenchMark XT; Ventana Medical Systems, Inc., Tucson, AZ, USA) and an alkaline phosphatase detection method (Enhanced Alkaline Phosphatase Detection Red Kit; Ventana) 16. Epitope retrieval was performed with Cell Conditioning 1 Solution (Ventana) at 96°C for 90 minutes, followed by rinsing in reaction buffer (Ventana).…”

Section: Methodsmentioning

confidence: 99%

Expression and activity of the urokinase plasminogen activator system in canine primary brain tumors

Rossmeisl¹,

Hall-Manning²,

Robertson³

et al. 2017

OTT

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BackgroundThe expression of the urokinase plasminogen activator receptor (uPAR), a glycosylphosphatidylinositol-anchored protein family member, and the activity of its ligand, urokinase-type plasminogen activator (uPA), have been associated with the invasive and metastatic potentials of a variety of human brain tumors through their regulation of extracellular matrix degradation. Domesticated dogs develop naturally occurring brain tumors that share many clinical, phenotypic, molecular, and genetic features with their human counterparts, which has prompted the use of the dogs with spontaneous brain tumors as models to expedite the translation of novel brain tumor therapeutics to humans. There is currently little known regarding the role of the uPA system in canine brain tumorigenesis. The objective of this study was to characterize the expression of uPAR and the activity of uPA in canine brain tumors as justification for the development of uPAR-targeted brain tumor therapeutics in dogs.MethodsWe investigated the expression of uPAR in 37 primary canine brain tumors using immunohistochemistry, Western blotting, real-time quantitative polymerase chain reaction analyses, and by the assay of the activity of uPA using casein–plasminogen zymography.ResultsExpression of uPAR was observed in multiple tumoral microenvironmental niches, including neoplastic cells, stroma, and the vasculature of canine brain tumors. Relative to normal brain tissues, uPAR protein and mRNA expression were significantly greater in canine meningiomas, gliomas, and choroid plexus tumors. Increased activity of uPA was documented in all tumor types.ConclusionsuPAR is overexpressed and uPA activity increased in canine meningiomas, gliomas, and choroid plexus tumors. This study illustrates the potential of uPAR/uPA molecularly targeted approaches for canine brain tumor therapeutics and reinforces the translational significance of canines with spontaneous brain tumors as models for human disease.

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Matrix metalloproteinases 2 and 9 expression in canine normal prostate and with proliferative disorders

Cited by 9 publications

References 36 publications

Characterization of Collagen Fibers (I, III, IV) and Elastin of Normal and Neoplastic Canine Prostatic Tissues

Characterization of Collagen Fibers (I, III, IV) and Elastin of Normal and Neoplastic Canine Prostatic Tissues

Characterization of Collagens Fibers (I, III, IV) and Elastin in the Extracellular Matrix of Normal and Neoplastic Canine Prostate

Expression and activity of the urokinase plasminogen activator system in canine primary brain tumors

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