Matrix metalloproteinases-2, -7 and tissue metalloproteinase inhibitor-1 expression in human endometrium

Abstract

The results of the study indicate that estradiol influence MMP-2 expression in the endometrium depends on the phase of menstrual cycle. Such relationships were not found for MMP-7 and TIMP-1 and further tests clarifying association between estradiol and MMPs are needed.

miR-29c overexpression and COL4A1 downregulation in infertile human endometrium reduces endometrial epithelial cell adhesive capacity in vitro implying roles in receptivity

“…MMP2 is a confirmed miR-29c target in lung cancer 18 . Its expression is reported to be highest in the fertile proliferative phase, suggesting a role in endometrial remodelling 28 . It has also been reported MMP2 expression during receptivity is dysregulated with infertility 29 .…”

Human Decellularized Endometrial Scaffold Seeded by Myometrial Smooth Muscle Cells Supports the Differentiation of Human Endometrial Mesenchymal Cells to Epithelial and Stromal Cells ​

“…In agreement, it is demonstrated that the cultured CD146 + human endometrial cells in the collagen/matrigel scaffold in the presence of MSMCs resulted in the expression of ZO-1, COL4A1, and LAMA2 genes at the mRNA level as well as the formation of glandular-like structures at the morphological and ultrastructural levels [19]. The osteopontin (SPP1) and matrixmetaloproteinase-2 (MMP2) proteins are secreted by the endometrial epithelial and stromal cells [27][28][29][30][31]. Osteopontin plays a role in embryo implantation, placenta formation, cell-to-cell adhesion, and proliferation and migration of endometrial cells [27][28][29] and the MMP proteases have an essential function in endometrial tissue remodeling during the menstrual cycle [30][31].…”

“…The osteopontin (SPP1) and matrixmetaloproteinase-2 (MMP2) proteins are secreted by the endometrial epithelial and stromal cells [27][28][29][30][31]. Osteopontin plays a role in embryo implantation, placenta formation, cell-to-cell adhesion, and proliferation and migration of endometrial cells [27][28][29] and the MMP proteases have an essential function in endometrial tissue remodeling during the menstrual cycle [30][31]. Myometrial cells secrete some factors, ECM components, and substances that play an inductive role in the epithelialization of human endometrial tissue as well as cell proliferation, differentiation, migration, attachment, polarity, and apoptosis [32].…”

Utilizing the Knowledge of Role of Matrix Matrixmetalloproteinases Along with Tissue Inhibitors of Matrix Metalloproteinasesin Various Gynaecological Disorders with Great Emphasis on Preeclampsia, PCOs Along with Gynaecological Malignancies-A Systematic Review

“…Further Grzechocinska et al demonstrated how there was an absence of association among lack of correlation between proMMP-2 along with MMP-2 amounts point that separate pathway of their activation in AUB. ProMMP-2 is up regulated by estradiol and down regulated by progesterone while MMP-2 levels increase with the length of bleeding [143]. This way with knowledge given by Johanns et al with regards to Cellular uptake of proMMP-2:TIMP-2 complexes by the endocytic receptor megalin/LRP-1 ,with earlier knowledge on how low-density lipoprotein receptor-related protein-1 (LRP-1) mediates the clearance of a complex between the zymogen form of MMP-2 (proMMP-2) and tissue inhibitor of metalloproteinases, TIMP-2, in HT1080 human fibrosarcoma cells .…”