Matrix metalloproteinase (MMP)-2 and MMP-9 in seminal plasma

Tentes, Ioannis; Asimakopoulos, Byron; Mourvati, Efthimia; Diedrich, K.; Al‐Hasani, S.; Nikolettos, Nikos

doi:10.1007/s10815-007-9129-6

Cited by 35 publications

(35 citation statements)

References 13 publications

(19 reference statements)

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“…Based on previous reports (Metayer et al 2002;Shimokawa et al 2002;McCauley et al 2001;Tentes et al 2007), the bands with 225, 78 and 66 kDa correspond to dimer-MMP-9, proMMP-2 and active MMP-2. The variation in molecular weights (by 10 to 15 kDa) of gelatinases of different origins can be result of different glycosylation (Metayer et al 2002).…”

Section: +mentioning

confidence: 60%

“…Similarly, in human seminal plasma a higher concentration of pro-MMP-9 was detected in semen with abnormally low sperm concentration (Tentes et al 2007). The same study reported that inactive or active forms of MMP-2 and MMP-9 do not correlate with semen indicators.…”

Section: +mentioning

confidence: 95%

“…The role of TIMPs for human sperm function is most probably based on interaction with MMPs in spermatozoa (Baumgart et al 2002). MMP-2 and MMP-9 were evaluated in human seminal plasma, where latent forms of both MMPs predominate, and pro-MMP-9 was detected in samples of low sperm concentration (Tentes et al 2007).…”

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confidence: 99%

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Gelatinases in Boar Seminal Plasma and Their Relation to Semen Indicators

et al. 2010

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Matrix metalloproteinases were detected in reproductive tissues and seminal plasma of various animal species. The aim of this study was to determine for the first time the presence of gelatinases and metalloproteases in boar seminal plasma and to correlate the results with semen indicators. Gelatin zymography was used for simultaneous identification and measurement of gelatinase enzyme activity associated with their molecular weights.Several gelatinase forms were identified in seminal plasma of boars. Those that were stimulated by CaCl 2 and inhibited by EDTA and phenanthroline were considered as metalloproteases. Negative correlation between semen indicators (sperm index, sperm concentration and concentration of progressive motile sperm) and the concentrations of metalloprotease at 78 kDa and 66 kDa means that higher values of semen indicators correlate with lower concentrations of these metaloproteases in seminal plasma. Gelatinases with molecular weight of 225, 78 and 66 kDa correlated with higher levels of acrosome damage. Samples with sperm index above 110 M/ml contained gelatinases of significantly lower band intensities at 78 and 66 kDa compared to samples with SI less than 110 M/ml. Bands with 225, 78 and 66 kDa are suggested to belong to a dimer of MMP-9, proMMP-2 and mature MMP-2. Matrix metalloproteinases, boar semen evaluation, semen characteristics

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Section: +mentioning

confidence: 60%

Section: +mentioning

confidence: 95%

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Gelatinases in Boar Seminal Plasma and Their Relation to Semen Indicators

et al. 2010

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“…Matrix metalloproteinases and their tissue inhibitors play a key role in many physiological processes, including ovulation, fertilization and implantation (Hulboy et al, 1997). Metalloproteases like MMP-2, MMP-9 and serine proteinases have been detected in turkey and human seminal plasma (Kotlowska et al, 2005) and their latent forms in canine seminal plasma and deteriorated semen samples with unsatisfactory quality traits (Tentes et al, 2007). The inactive forms were inversely correlated with semen quality and active forms were positively correlated with semen quality traits and sperm functionality (Saengsoi et al, 2011).…”

Section: Proteases and Proteases Inhibitors Of Seminal Plasmamentioning

confidence: 99%

Proteases and Proteases Inhibitors of Semen - A Review

Gurupriya¹

2014

Adv. Anim. Vet. Sci.

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| The ejaculate composition is extremely complicated and variable among livestock species. The seminal proteins and enzymes are vital for ejaculate metabolism, spermatozoa performance, survival, and transport within the feminine reproductive tract. Proteases and proteinase inhibitors are secreted by the accessory sex glands of the male reproductive tract and find mixed with the spermatozoa throughout ejaculation. But an entire understanding of those enzymes and their performance in numerous class species is not obtainable. The performance of the assorted proteinases and protease inhibitors of ejaculate stay a mystery, and only a few studies are conducted concerning the characterization of those enzymes. Throughout this review, we tend to target the current understanding of the key proteases and their inhibitors of ejaculate from various mammalian species.

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“…So far, only the existence of two MMPs, namely MMP-2 and MMP-9, has been reported in human seminal plasma (Baumgart et al, 2002;Buchman-Shaked et al, 2002;Shimokawa Ki et al, 2002;Tentes et al, 2007). However, correlation of these results with semen parameters led to contradictory findings.…”

Section: Introductionmentioning

confidence: 99%

Macrophage metalloelastase-12 is detectable in human seminal plasma and represents a predictor for inflammatory processes in the male genital tract

et al. 2014

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Macrophage metalloelastase-12 (MMP-12), a protein of the matrix metalloproteinase family, is involved in the breakdown of extracellular matrix in normal physiological processes as well as in disease processes. MMP-12 is almost exclusively produced by macrophages and is associated with inflammatory disorders. Giving the fact that inflammation negatively influences ejaculate parameters, we investigated a possible presence and correlation of MMP-12 in seminal plasma with parameters of the ejaculate, especially in leucocytospermic ejaculates. Forty-two patients who presented for semen analysis were assigned into four groups depending on the result of semen analysis according to the WHO guidelines 2010: normozoospermia (n = 11), OAT (n = 10), azoospermia (n = 10) and leucocytospermia (>1 mio. peroxidase-positive cells per ml) (n = 11). MMP-12 was detected by ELISA and was measurable in nearly all seminal plasma samples. Generally, MMP-12 concentrations were significantly higher in leucocytospermic samples than in nonleucocytospermic ones (P = 0.001). The MMP-12 levels between the latter nonleucocytospermic groups did not differ. Moreover, MMP-12 levels correlated with the presence of peroxidase-positive leucocytes. No correlation with CD 14 positive monocytes/macrophages was detected. In this study, we demonstrate that MMP-12 is present in seminal plasma and is correlated with inflammatory conditions in human semen and therefore may serve as predictor of ongoing inflammatory processes.

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Matrix metalloproteinase (MMP)-2 and MMP-9 in seminal plasma

Cited by 35 publications

References 13 publications

Gelatinases in Boar Seminal Plasma and Their Relation to Semen Indicators

Gelatinases in Boar Seminal Plasma and Their Relation to Semen Indicators

Proteases and Proteases Inhibitors of Semen - A Review

Macrophage metalloelastase-12 is detectable in human seminal plasma and represents a predictor for inflammatory processes in the male genital tract

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