Matrix metalloproteinase inhibition impairs the processing, formation and mineralization of dental tissues during mouse molar development

Bourd-Boittin, Katia; Fridman, Rafael; Fanchon, Stéphanie; Septier, D.; Goldberg, Michel; Ménashi, Suzanne

doi:10.1016/j.yexcr.2004.11.024

Cited by 83 publications

(78 citation statements)

References 48 publications

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“…Degradation of exposed collagen fibrils within hybrid layers has been accepted as a key factor in the deterioration of the adhesivedentin interface [15]. Recent studies reported that degradation is expedited by the presence of endogenous proteases present in dentin such as MMPs [7,15,25]. Dentin MMP preforms are activated during acid-etching, being activated by low pH and the presence of metal ions such as zinc and calcium [14,26].…”

Section: Discussionmentioning

confidence: 99%

“…These agents include the use of substances that are considered to be inhibitors of matrix metalloproteinases (MMPs) and cysteine cathepsins. Different types of MMPs have been identified from human dentin, including MMP-2, MMP-3, MMP-8, MMP-9, MMP-20 [7][8][9]. More recently, different types of cysteine cathepsins, in particular cathepsin B, have also been identified from sound and carious dentin [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

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New Trends in Dentin Bonding: Treatment with chlorhexidine, hyaluronic acid, vitaminc C and green tea

Fonseca

Pleffken

Balducci

et al. 2013

BDS

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Objective: The aim of this study was to evaluate the bond strength of dentin treated with chlorhexidine, hyaluronic acid, vitamin C and green tea. Material and Methods: The roots of 50 bovine teeth were removed and buccal coronal dentin was exposed. After acid-etching, the specimens were divided into 5 groups (n = 10), according to the dentin treatment strategy: CO - untreated dentin; CHX - treated with 2 wt% chlorhexidine for 30 sec; HA - treated with 1 wt% hyaluronic acid for 30 sec; VC - treated with 10 wt% vitamin C for 30 sec; GT - treated a 1% green tea extract for 30 sec. Adper Single Bond was then applied to the treated according to the manufacturer's recommendations. The specimens were restored with a 4-mm thick layer of the resin composite, which was polymerized for 40 sec. The specimens were stored in distilled water at 37°C for 24 h and sectioned into 1x1 mm2 sticks containing the adhesive interface. Microtensile bond strength testing was performed with a universal testing machine at a cross-head speed of 1.0 mm/min. Results: The results were analyzed with one-factor ANOVA and Tukey’s multiple comparison tests. GT group presented the highest values bond strength (29.4±3.1)a, but no significant difference compared to the other experimental groups HA(26.7±3.1)ab, CHX(25.4±2.6)ab and VC(22.4±6.0)b. Bond strengths of experimental groups were not significantly different from the CO. Conclusion: Immediate bond strength was preserved after acid-etched dentin was treated.Keywords: Chlorhexidine. Hyaluronic acid. Vitamin C. Green tea. Bond strength.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

New Trends in Dentin Bonding: Treatment with chlorhexidine, hyaluronic acid, vitaminc C and green tea

Fonseca

Pleffken

Balducci

et al. 2013

BDS

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“…3 Human dentin contains at least five types of MMPs including MMP-2, MMP-3, MMP-8, MMP-9, and MMP-20. [4][5][6][7][8][9] They are trapped within the mineralized dentin matrix during tooth development 5 and dentinal caries. 8 The majority of them are produced as latent zymogens (pro-MMPs).…”

Section: Introductionmentioning

confidence: 99%

“…Disruption of MMP-collagen binding due to exposure to mild acids (pH¼2. [3][4][5] 8 converts the pro-MMPs to active MMPs through splitting the low-molecularweight peptides. 10,11 Over time, auto activation of further pro-MMPs may result in increasing the enzymatic activity.…”

Section: Introductionmentioning

confidence: 99%

Effect of Chlorhexidine Pretreatment on Bond Strength Durability of Caries–affected Dentin Over 2-Year Aging in Artificial Saliva and Under Simulated Intrapulpal Pressure

Mobarak¹

2011

Operative Dentistry

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Pretreatment of caries-affected dentin with 5% chlorhexidine decelerates the bond strength loss over the long term. SUMMARYObjective: To evaluate the influence of 2% and 5% chlorhexidine (CHX) pretreatment on bond durability of a self-etching adhesive to normal (ND) and caries-affected (AD) dentin after 2-years of aging in artificial saliva and under simulated intrapulpal pressure (IPP).Methods: One hundred twenty freshly extracted carious teeth were ground to expose ND and AD. Specimens were distributed into three equal groups (n¼40) according to whether the dentin substrates were pretreated with 2% or 5% CHX or with water (control). Clearfil SE Bond (Kuraray) was applied to both substrates and composite cylinders (0.9 mm diameter and 0.7 mm height) were formed. Pretreatment and bonding were done while the specimens were subjected to 15 mm Hg IPP. After curing, specimens were aged in artificial saliva at 378C and under IPP at 20 mm Hg until being tested after 24 hours or 2 years (n¼20/group). Microshear bond strength was evaluated. Failure modes were determined using a scanning electron microscope (SEM) at 4003 magnification. Data were statistically analyzed using three-way analysis of variance (ANOVA); oneway ANOVA tests, and t-test (p,0.05). Additional specimens (n¼5/group) were prepared to evaluate interfacial silver precipitation.Results: For the 24-hour groups, there were no significant differences among the ND groups and AD groups. For ND aged specimens, the 5% CHX group had the highest value followed by the 2% CHX and control groups, although the difference was statistically insignificant. For AD aged specimens, the 5% CHX group revealed statistically higher bond values compared to the 2% CHX and control groups. Fracture modes were predominately adhesive and mixed. Different interfacial silver depositions were recorded.Conclusions: Two percent or 5% CHX pretreatment has no adverse effect on the 24-hour bonding to ND and AD. Five percent CHX was able to diminish the loss in bonding to AD after 2years of aging in artificial saliva and under simulated IPP.

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“…For example, DMP-1 is physiologically processed by BMP-1/Tolloid-like proteinases 31) . DSP and amelogenin are substrates for MMP-2 32) . Den- ocation avoids interference with the pulp horns, which are biologically different in the enamel free area from the rest of the pulp chamber.…”

Section: Dentin Matrix Proteins Composition Functions and Bioactive mentioning

confidence: 99%

Bioactive Molecules Stimulate Tooth Repair and Regeneration

Goldberg¹,

Lacerda-Pinheiro²,

Jegat³

et al. 2006

J. Hard Tissue Biology.

Self Cite

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Abstract:Dentin extracellular matrix proteins display multifunctional properties. Firstly, they participate to the mineralization processes either as promotors or as inhibitors of crystal nucleation or crystal growth. Secondly, they act as signaling molecules implicated in the differentiation of odontoblast progenitors. These molecules may be used to promote the recruitment of odontoblast progenitors, the proliferation and the final differentiation into functional odontoblast-like or osteoblast-like cells implicated in pulp repair. This has been evaluated through a series of experiments carried out in vivo on the rat first maxillary molar and in vitro on odonto/osteo progenitors. Along this line, BMP7 (OP1) induced in the crown a fibrous osteodentin-like structure where unmineralized pulp remnants were seen. In addition, the mesial root canal was totally filled with a homogeneous dentin-like structure. The bone sialoprotein (BSP) stimulated within one month the formation of a reparative dentinal bridge and the complete closure of the coronal pulp with an atubular homogeneous reparative dentin. Dentonin, a peptide from MEPE, implantated into the exposed pulp produced more rapidly than the two previous molecules reparative mineralization in the coronal pulp and also occlusion of the lumen of the root canal. Implanted in the exposed pulp, A+4 and A-4, two spliced amelogenin gene products, induce either the formation of a reparative dentinal bridge (A+4) or a more diffuse mineralization (A-4). The mechanisms of proliferation and differentiation were studied in parallel in an in vivo situation after implantation in the first maxillary molar of the rat, and in vitro on odontoblast progenitor cell lines. These molecules may contribute to pulp repair and promote new strategies in dental therapies.

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Matrix metalloproteinase inhibition impairs the processing, formation and mineralization of dental tissues during mouse molar development

Cited by 83 publications

References 48 publications

New Trends in Dentin Bonding: Treatment with chlorhexidine, hyaluronic acid, vitaminc C and green tea

New Trends in Dentin Bonding: Treatment with chlorhexidine, hyaluronic acid, vitaminc C and green tea

Effect of Chlorhexidine Pretreatment on Bond Strength Durability of Caries–affected Dentin Over 2-Year Aging in Artificial Saliva and Under Simulated Intrapulpal Pressure

Bioactive Molecules Stimulate Tooth Repair and Regeneration

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