Matrix metalloproteinase-9 activity in human lens epithelial cells of cortical, posterior subcapsular, and nuclear cataracts

Alapure, Bhagwat V.; Praveen, Mamidipudi R.; Gajjar, Devarshi; Vasavada, Abhay R.; Sankaranarayanan, Rajkumar; Johar, Kaid

doi:10.1016/j.jcrs.2008.08.016

Cited by 23 publications

(31 citation statements)

References 51 publications

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“…On the other hand, increased activity of MMP-9 and MMP-2 was observed in lens epithelial cells (LECs) of patients with age-related cataract or in aqueous humour (AH) and uvea in animal models [6,7,24]. It suggests that OA or UA activity decreasing MMP levels in eye tissues might be an interesting alternative in the treatment of ocular diseases.…”

Section: Oa and Ua Impact On Production Of Metalloproteinases (Mmp-2 mentioning

confidence: 99%

The impact of oleanolic and ursolic acid on corneal epithelial cells in vitro

et al. 2017

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introduCtion. Oleanolic (OA) and ursolic (UA) acids belong to the triterpene group widely present in plants. These compounds are recognised to have anti-inflammatory properties and thus are considered to be used in therapies as well as in cosmetic, natural health, or diet products. aiM. The scientific hypothesis of our study was to show that OA and UA influence corneal epithelial cells cultured in vitro. Methods. Toxicity tests, based on MTT and Neutral Red (NR) uptake, measurement of nitric oxide (NOx) level, as well as analysis of metalloproteinases (MMP-2 and MMP-9) amount and activity were performed. resuLts. UA expressed significantly higher toxicity on cells than OA. At the lowest concentration applied (5 µM), UA limited cellular metabolism and viability on average by 22% as compared to untreated control, while 25 µM resulted in values lower than 10%. On the other hand, OA at the highest (100 µM) concentration limited cellular metabolism and viability by about 20%. NOx level significantly increased when OA and UA were applied at concentrations of 25 and 100 µM, respectively. OA and UA had a stronger impact on the level of MMP-2 than MMP-9. OA and UA reduced MMP-2 and MMP-9 in the whole range of concentrations. Tested triterpenoids had no significant impact on MMP activity. ConCLusions. OA and UA have a different impact on human corneal epithelial cells. UA is toxic for corneal epithelial cells, while OA exhibits milder activity, which may be useful for further analysis in ocular pharmacology.

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Section: Oa and Ua Impact On Production Of Metalloproteinases (Mmp-2 mentioning

confidence: 99%

The impact of oleanolic and ursolic acid on corneal epithelial cells in vitro

et al. 2017

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“…In glaucomatous eyes, alterations in MMPs are considered to contribute to the increased outflow resistance [96], and increased activity of MMP-9 has an important role in the corneal stroma pathology observed in the dysfunctional tear state [97]. Furthermore, high MMP-9 activity is observed in the eyes with cortical cataract, and the activity increases with age in the lens epithelial cells of patients with age-related cataract [98]. Thus, there is a great deal of evidence that MMPs play important roles in the pathogenesis of other ocular diseases.…”

Section: Mmps and Other Ocular Diseasesmentioning

confidence: 99%

Matrix metalloproteinases in diabetic retinopathy: potential role of MMP-9

Kowluru

Zhong

Santos

2012

Expert Opinion on Investigational Drugs

140

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Introduction Diabetic retinopathy remains one of the most feared complications of diabetes. Despite extensive research in the field, the molecular mechanism responsible for the development of this slow progressing disease remains unclear. In the pathogenesis of diabetic retinopathy, mitochondria are damaged and inflammatory mediators are elevated before the histopathology associated with the disease can be observed. Matrix metalloproteinases (MMPs) regulate a variety of cellular functions including apoptosis and angiogenesis. Diabetic environment stimulates the secretion of several MMPs that are considered to participate in complications, including retinopathy, nephropathy and cardiomyopathy. Patients with diabetic retinopathy and also animal models have shown increased MMP-9 and MMP-2 in their retina and vitreous. Recent research has shown that MMPs have dual role in the development of diabetic retinopathy; in the early stages of the disease (pre-neovascularization), MMP-2 and MMP-9 facilitate the apoptosis of retinal capillary cells, possibly via damaging the mitochondria, and in the later phase, they help in neovascularization. Areas covered This article reviews the literature to evaluate the role of MMPs, especially MMP-9, in the development of diabetic retinopathy, and presents existing evidence that the inhibitors targeted toward MMP-9, depending on the duration of diabetes at the times their administration could have potential to prevent the progression of this blinding disease, and protect the vision loss. Expert opinion Inhibitors of MMPs could have dual role: in the early stages of the diseases, inhibit capillary cell apoptosis, and if the disease has progressed to the angiogenic stage, inhibit the growth of new vessels.

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“…Lens proteases are involved in morphogenesis and in maintaining the clarity of the lens by degrading oxidized and modified proteins and peptides (3)(4)(5)(6). Several proteolytic enzymes have been shown to play a role in lens aging and cataract formation (7)(8)(9)(10)(11)(12)(13). Human cataractous lenses have increased proteolysis and protein aggregation as compared with age-matched noncataractous lenses (14,15).…”

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confidence: 99%

Lens Crystallin Modifications and Cataract in Transgenic Mice Overexpressing Acylpeptide Hydrolase

Santhoshkumar¹,

Xie

Raju

et al. 2014

Journal of Biological Chemistry

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Background: Acylpeptide hydrolase (APH) in the lens may have an integral role in cataract formation. Results: Transgenic overexpression of APH results in crystallin cleavage, impaired lens development, and cataract. Conclusion: APH may be involved in the generation of peptides that have the potential to induce protein aggregation. Significance: The transgenic APH mouse model could help us understand the role of crystallin fragments in cataractogenesis.

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Matrix metalloproteinase-9 activity in human lens epithelial cells of cortical, posterior subcapsular, and nuclear cataracts

Abstract: A significant difference was observed in the level of MMP-9 activity between different types of cataract. The activity of MMP-9 was highest in eyes with cortical cataract. The level of MMP-9 activity increased with age in the LECs of patients with age-related cataract.

Cited by 23 publications

References 51 publications

The impact of oleanolic and ursolic acid on corneal epithelial cells in vitro

The impact of oleanolic and ursolic acid on corneal epithelial cells in vitro

Matrix metalloproteinases in diabetic retinopathy: potential role of MMP-9

Lens Crystallin Modifications and Cataract in Transgenic Mice Overexpressing Acylpeptide Hydrolase

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