Matrix metalloproteinase-2 is involved in A549 cell migration on laminin-10/11

Gu, Jianxin; Nishiuchi, Ryoko; Sekiguchi, Kiyotoshi

doi:10.1016/s0006-291x(02)00831-8

Cited by 11 publications

(9 citation statements)

References 31 publications

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“…We also observed that intestinal epithelial cells migrate towards laminin-511, but this occurs in a PI3K-independent manner. The described role of laminin-511 in cell migration is in agreement with previously published data [42], [43] and may support its important role in metastasis [44], [45].…”

Section: Discussionsupporting

confidence: 91%

Abnormal Wnt and PI3Kinase Signaling in the Malformed Intestine of lama5 Deficient Mice

et al. 2012

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Laminins are major constituents of basement membranes and are essential for tissue homeostasis. Laminin-511 is highly expressed in the intestine and its absence causes severe malformation of the intestine and embryonic lethality. To understand the mechanistic role of laminin-511 in tissue homeostasis, we used RNA profiling of embryonic intestinal tissue of lama5 knockout mice and identified a lama5 specific gene expression signature. By combining cell culture experiments with mediated knockdown approaches, we provide a mechanistic link between laminin α5 gene deficiency and the physiological phenotype. We show that laminin α5 plays a crucial role in both epithelial and mesenchymal cell behavior by inhibiting Wnt and activating PI3K signaling. We conclude that conflicting signals are elicited in the absence of lama5, which alter cell adhesion, migration as well as epithelial and muscle differentiation. Conversely, adhesion to laminin-511 may serve as a potent regulator of known interconnected PI3K/Akt and Wnt signaling pathways. Thus deregulated adhesion to laminin-511 may be instrumental in diseases such as human pathologies of the gut where laminin-511 is abnormally expressed as it is shown here.

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Section: Discussionsupporting

confidence: 91%

Abnormal Wnt and PI3Kinase Signaling in the Malformed Intestine of lama5 Deficient Mice

et al. 2012

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“…Secretion of MMP-9 has been reported in lung, colon, and breast cancers [30] and MMP-2 expression has previously been found in A549 cells [31]. It was reported that even when A549 cells were treated with fibronectin (a highmolecular-weight glycoprotein having the ability to stabilize the attachment of ECM to cells) and laminin-10/11 (a major noncollagenous component of the basal lamina), the activity of MMP-9 still was not significantly enhanced [32]. Hence, the literature regarding the invasive and metastatic activity of A549 cells has focused almost exclusively on the activity of MMP-2 [33,34].…”

Section: Discussionmentioning

confidence: 96%

Resveratrol analog‐3,5,4′‐trimethoxy‐trans‐stilbene inhibits invasion of human lung adenocarcinoma cells by suppressing the MAPK pathway and decreasing matrix metalloproteinase‐2 expression

Yang

Weng

et al. 2009

Molecular Nutrition Food Res

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Resveratrol (3,5,4'-trihydroxystilbene) is a natural polyphenol that presents various physiological activities. It has been reported that the methylated derivatives of resveratrol show better potential antifungal and antiproliferative activities than resveratrol. In the present study, we investigated the inhibitory effect of 3,5,4'-trimethoxy-trans-stilbene (MR-3), a methylated derivative of resveratrol, on the invasion of A549 cells (a human lung adenocarcinoma cell line). We found that treatment with MR-3 at the concentration of 5 muM resulted in antiadhesive, antimigratory, and antiinvasive activities on A549 cells through the suppression of matrix metalloproteinase (MMP)-2 protein expression and transcriptional levels in a time-dependent manner. The suppression of MMP-2 expression by MR-3 led to an inhibition of A549 cell invasion by inactivating phosphorylation of SAPK/c-Jun N-terminal kinase (JNK) and p38 MAPK signaling pathways. A time-dependent inhibition of protein levels for p65, c-Jun, and c-Fos in the nucleus by MR-3 treatment was also observed. In conclusion, our data demonstrate that the antiinvasive effects of MR-3 on A549 cells are likely mediated through the inhibition of phosphorylation of JNK and p38, as well as a reduction in the protein levels of nuclear factor-kappa B (NF-kappaB) and activator protein-1 (AP-1) in the nucleus, ultimately leading to downregulation of MMP-2 expression.

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“…Laminin also induces MMP-2 expression and enhances tumor invasion (Gu et al 2002a;Reich et al 1995). Moreover, there are several interesting reports regarding MMP upregulation and invasion by other laminin isoforms (DiPersio et al 2000;Gu et al 2002b).…”

Section: Introductionmentioning

confidence: 99%

Laminin induces matrix metalloproteinase-9 expression and activation in human cervical cancer cell line (SiHa)

Maity

Sen

Chatterjee

2010

J Cancer Res Clin Oncol

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Matrix metalloproteinase-2 is involved in A549 cell migration on laminin-10/11

Cited by 11 publications

References 31 publications

Abnormal Wnt and PI3Kinase Signaling in the Malformed Intestine of lama5 Deficient Mice

Abnormal Wnt and PI3Kinase Signaling in the Malformed Intestine of lama5 Deficient Mice

Resveratrol analog‐3,5,4′‐trimethoxy‐trans‐stilbene inhibits invasion of human lung adenocarcinoma cells by suppressing the MAPK pathway and decreasing matrix metalloproteinase‐2 expression

Laminin induces matrix metalloproteinase-9 expression and activation in human cervical cancer cell line (SiHa)

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