Matrix metalloproteinase-2 and its correlation with basal membrane components laminin-5 and collagen type IV in paediatric burn patients measured with Surface Plasmon Resonance Imaging (SPRI) biosensors

Weremijewicz, Artur; Matuszczak, Ewa; Sankiewicz, Anna; Tylicka, Marzena; Komarowska, Marta; Tokarzewicz, Anna; Dębek, Wojciech; Gorodkiewicz, Ewa; Hermanowicz, Adam

doi:10.1016/j.burns.2017.12.001

Cited by 20 publications

(18 citation statements)

References 36 publications

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“…MMP‐1 is expressed by many cells, such as fibroblasts, macrophages, endothelium, and epithelium; the main function of MMP‐1 is to degrade collagen I, II, III, VII, X, and XI 28 . MMP‐2 mainly degrades type IV collagen 29 . It was found that MMP‐1 expression was significantly decreased in a rabbit model of pBOO, while TIMP1 expression were significantly increased 30 .…”

Section: Discussionmentioning

confidence: 98%

“…28 MMP-2 mainly degrades type IV collagen. 29 It was found that F I G U R E 7 β-Adrenoceptor agonists promote MMP1 and MMP2 expression in HUCs under 70 cm H 2 O HP via PKA/cAMP response element binding protein (CREB) pathways. A, Formoterol (For) and BRL 37344 (BRL), β 2and β 3 -adrenoceptor agonists, respectively, remarkably increased the protein levels of p-CREB in HUCs after exposure to 70 cm H 2 O HP for 6 hours compared with the control group.…”

Section: Discussionmentioning

confidence: 99%

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β‐Adrenoceptors regulate matrix metalloproteinase expression in human urothelial cells under hydrostatic pressure

Lan

Jin

et al. 2020

Neurourology and Urodynamics

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The bladder wall is constantly subjected to intravesical pressure during the filling and voiding cycles. An imbalance between matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) under elevated intravesical pressure contributes to pathological changes in the bladder. To investigate the changes in human urothelial cells (HUCs) under elevated intravesical pressure, this study analyzed the effect of β‐adrenoceptor signaling on the expression of MMPs and TIMPs in HUCs exposed to pathological hydrostatic pressure (HP) (70 cm H2O) for 6 hours. Quantitative polymerase chain reaction, Western blot analysis, and cell fluorescence staining were used to explore the effect of β‐adrenoceptor signaling on the expression of MMPs and TIMPs in HUCs after agonist and/or antagonist treatment. The expression levels of β2‐ and β3‐adrenoceptor, MMP1, and MMP2 were greatly downregulated, while the expression of TIMP1 was greatly upregulated. Formoterol and BRL 37344, which are agonists of β2‐ and β3‐adrenoceptor, respectively, significantly increased MMP1 and MMP2 expression under 70 cm H2O. As a classic downstream pathway of β2‐ and β3‐adrenoceptor, protein kinase A (PKA) signaling inhibited MMP1 and MMP2 expression by regulating cAMP response element binding protein (CREB) activity. MMP1 and MMP2 expression in HUCs under 70 cm H2O was modified by β2‐ and β3‐adrenoceptor via the PKA/CREB pathway. This outcome suggests that MMPs likely participate in the pathological effects of elevated intravesical pressure. The underlying mechanism of β2‐ and β3‐adrenoceptor in elevated intravesical pressure was also revealed; this mechanism constitutes a new potential therapeutic target for partial bladder outlet obstruction.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

β‐Adrenoceptors regulate matrix metalloproteinase expression in human urothelial cells under hydrostatic pressure

Lan

Jin

et al. 2020

Neurourology and Urodynamics

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“…Some studies report the protein ubiquitin C-terminal hydrolase L1 (UCHL-1) as a novel potential biomarker of brain injury [ 40 , 41 , 42 ]. Our previous studies show that in children, UCHL-1 is overexpressed in cases of cryptorchidism, acute appendicitis and thermal injury [ 43 , 44 , 45 , 46 ]. Brophy et al [ 40 ] as well as Mondello et al [ 47 ] demonstrated increased serum UCHL-1 concentration in adults after traumatic brain injury.…”

Section: Discussionmentioning

confidence: 99%

BDNF and IL-8, But Not UCHL-1 and IL-11, Are Markers of Brain Injury in Children Caused by Mild Head Trauma

et al. 2020

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The aim of the study was to check whether the plasma levels of brain-derived neurotrophic factor (BDNF), interleukin-8 (IL-8), interleukin-11 (IL-11) and ubiquitin C-terminal hydrolase L1 (UCHL-1) change in children with mild head trauma (N = 29) compared to controls (N = 13). Protein concentration in children with mild head trauma (12 children with mild concussion without loss of consciousness and 17 children with severe concussion and loss of consciousness) and the control group were measured by means of the Enzyme-Linked Immunosorbent Assay (ELISA) method. IL-8 and BDNF concentration was statistically higher in the group of children with mild head trauma (9.89 pg/mL and 2798.00 pg/mL, respectively) compared to the control group (7.52 pg/mL and 1163.20 pg/mL, respectively). BDNF concentration was significantly higher in children with severe concussion and loss of consciousness (3826.00 pg/mL) than in the control group. None of the tested proteins differed significantly between children with mild concussion without loss of consciousness and children with severe concussion and loss of consciousness. BDNF and IL-8 may be sensitive markers of brain response to mild head trauma in children. The lack of statistical differences for BDNF and IL-8 between children with mild or severe concussion could indicate that their elevated levels may not result from significant structural brain damage but rather reflect a functional disturbance.

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“…Reproduced with permission from [71]. SPR sensors have also been used in diverse healthcare biosensing applications, bioimaging paediatric burns [75] as well as in the study of mutations [76] and the effects of radiation exposure [77]. Recent studies have also shown the versatility of such devices in the study of pathogens [78], thermal injuries [79], carcinogens [80] and several clinically vital molecules [81][82][83].…”

Section: Localised Surface Plasmon Resonance Sensors For Biomedical Dmentioning

confidence: 99%

Recent Progress in Optical Sensors for Biomedical Diagnostics

Pirzada

Altıntaş

2020

Micromachines

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In recent years, several types of optical sensors have been probed for their aptitude in healthcare biosensing, making their applications in biomedical diagnostics a rapidly evolving subject. Optical sensors show versatility amongst different receptor types and even permit the integration of different detection mechanisms. Such conjugated sensing platforms facilitate the exploitation of their neoteric synergistic characteristics for sensor fabrication. This paper covers nearly 250 research articles since 2016 representing the emerging interest in rapid, reproducible and ultrasensitive assays in clinical analysis. Therefore, we present an elaborate review of biomedical diagnostics with the help of optical sensors working on varied principles such as surface plasmon resonance, localised surface plasmon resonance, evanescent wave fluorescence, bioluminescence and several others. These sensors are capable of investigating toxins, proteins, pathogens, disease biomarkers and whole cells in varied sensing media ranging from water to buffer to more complex environments such as serum, blood or urine. Hence, the recent trends discussed in this review hold enormous potential for the widespread use of optical sensors in early-stage disease prediction and point-of-care testing devices.

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Matrix metalloproteinase-2 and its correlation with basal membrane components laminin-5 and collagen type IV in paediatric burn patients measured with Surface Plasmon Resonance Imaging (SPRI) biosensors

Cited by 20 publications

References 36 publications

β‐Adrenoceptors regulate matrix metalloproteinase expression in human urothelial cells under hydrostatic pressure

β‐Adrenoceptors regulate matrix metalloproteinase expression in human urothelial cells under hydrostatic pressure

BDNF and IL-8, But Not UCHL-1 and IL-11, Are Markers of Brain Injury in Children Caused by Mild Head Trauma

Recent Progress in Optical Sensors for Biomedical Diagnostics

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