Matrix metalloproteinase-2, -9, and tissue inhibitor of MMP-2 gene polymorphisms in Taiwanese periodontitis patients

Abstract

Background/purpose Matrix metalloproteinases (MMPs) and tissue inhibitor of MMPs (TIMPs) have been shown to play an important role in the pathogenesis of tissue destruction in periodontitis. The associations between single nucleotide polymorphisms (SNPs) in the promoter regions of MMP-2, MMP-9, and TIMP-2 genes and the risk of aggressive periodontitis (AgP) and chronic periodontitis (CP) were investigated in a Taiwanese population. Materials and methods MMP-2 C-1306T, C-735T, T-790G, and MMP-9 C-1562T and TIMP-2 G-418C SNPs were genotyped by polymerase chain reaction-restriction fragment length polymorphism analysis in 69 patients and 129 patients with AgP and CP, respectively, and 117 periodontal healthy individuals who served as healthy controls (HC). Chi-square test and logistic regression analysis were used to investigate the possible association of genotypes with periodontitis. Results No significant differences in the distributions of the C-1306T and C-735T variants between periodontitis and HC were detected. Patients with genotype of MMP-2 -790 TT or T allele of MMP-2-790T/G as compared to genotypes of GT + GG genotypes or G allele, were less susceptible to CP [odds ratio (OR) = 0.50, 95% confidence interval (CI) = 0.25–1.00 and OR = 0.52, 95% CI = 0.28–0.96, respectively]. The frequencies of TIMP-2 G-418C gene polymorphisms in nonsmokers were statistically significantly different among AgP, CP, and HC groups (P = 0.024). The nonalcohol drinking participants with C allele of MMP-9 C-1562T as compared to T allele, were less susceptible to AgP (adjusted OR = 0.4; 95% CI, 0.18–0.90). Conclusion It is suggested that MMP-2 T-790G, MMP-9 C-1562T, and TIMP-2 G-418C gene polymorphisms might be associated with periodontitis in the Taiwanese Han population.

Matrix metalloproteinase-9-1562 C/T promoter gene polymorphism in chronic periodontitis: A cross-sectional observational study

“…Similar reports were observed by quite a few authors who have performed studies in Asian and Mixed populations like de Souza et al in Brazilian (mixed) population, Loo et al in Chinese (Asian) population, and several others. [ 13 35 40 41 42 43 44 45 ] On the other hand, the studies conducted in Caucasians have reported an association between MMP-9 SNP and CP; for instance, a study by Keles et al has found the association in Turkish (Caucasian) population which is in contrast with the current observation. [ 46 ] Meta-analyses by Pan et al and Mashhadiabbas et al have also found a moderate association in Caucasian population but nothing significant in Asian/mixed population.…”

Association of MMP-2 -753C>T and MMP-9 -1562C>T Polymorphisms with Chronic/Aggressive Periodontitis Risk: A Systematic Review and Meta-Analysis

“…Of these case-control studies, for the MMP-9 -1562C>T polymorphism, 13 case-control studies in ten publications (18–28) were available, including 1396 cases and 1709 controls. For the MMP-2 -753C>T polymorphism, six case-control studies (19,21–23,27) involved a total of 693 cases and 636 controls. Among the 13 eligible studies for MMP-9 -1562C>T (rs3918242), four case-control studies (19,20,22,23) including 438 cases and 469 controls were undertaken in Caucasians (Czech and Turkey), seven case-control studies (21,24,26–28) containing 827 cases and 1144 controls were conducted in Asians (China, Taiwan, and India), and two case-control studies (18,25) with 131 cases and 92 controls was performed in Latinos populations (Brazil and Colombia).…”

“…Among the 13 eligible studies for MMP-9 -1562C>T (rs3918242), four case-control studies (19,20,22,23) including 438 cases and 469 controls were undertaken in Caucasians (Czech and Turkey), seven case-control studies (21,24,26–28) containing 827 cases and 1144 controls were conducted in Asians (China, Taiwan, and India), and two case-control studies (18,25) with 131 cases and 92 controls was performed in Latinos populations (Brazil and Colombia). Of six case-control studies for MMP-2-753C>T (rs2285053), three case-control studies (19,22,23) including 328 cases and 391 controls were undertaken in Caucasians (Czech and Turkey) and three case-control studies (21,27) with 365 cases and 245 controls were performed in Asian (China and Taiwan) populations. The control populations of four studies deviated from Hardy–Weinberg equilibrium (HWE).…”

“…Recently, two meta-analyses estimated the association between MMP-2 -753C>T and MMP- 9 -1562C>T polymorphisms and periodontitis risk, which was basically in accordance with our results that MMP-2 -753C>T and MMP-9 -1562C>T polymorphisms may not contribute to the susceptibility of periodontitis in overall analysis (8,11). Moreover, at least four case-control studies (in two publications) (27,28) have not included in the meta-analysis (8). Additionally, Li et al have included only two studies for MMP-2 -753C>T and MMP-9 -1562C>T polymorphisms (11).…”

The relationship between periodontal status and rheumatoid arthritis – systematic review

“…Especially gelatinases, including the MMP-2 and MMP-9 enzymes, play a crucial role in hemostasis and the inflammatory response. Gene polymorphisms of metalloproteinases and their expression changes are observed in both PD and RA [ 42 , 43 ].…”