Matrix Metallopeptidase 14 Plays an Important Role in Regulating Tumorigenic Gene Expression and Invasion Ability of HeLa Cells

Zhang, Yinghui; Wang, Sheng; Li, Min; Zheng, Hanxi; Xu, Lan; Chen, Youguo

doi:10.1097/igc.0000000000000652

Cited by 4 publications

(3 citation statements)

References 31 publications

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“…Furthermore, we examined the expression of MMP14 in 60 cervical cancer tissues and 60 adjacent normal tissues. The expression of MMP14 in cervical cancer tissues was much higher than in normal cervical tissues (Figure 3(e,f)), indicating that high expression of MMP14 is associated with cervical cancer, which was consistent with other researchers [22,26]. These data indicate that the CCAT1 expression level is negatively correlated with miR-181a-5p and further regulates the expression of MMP14.…”

Section: Ccat1 Regulates the Expression Of Mir-181a-5p And Matrix Metsupporting

confidence: 89%

“…The matrix metalloproteinase (MMP) family proteins are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes. MiR-181a-5p is reported to inhibit cell proliferation and invasion by downregulating MMP14 [22,26]. Additionally, MMP14 activity mediates the proteolytic processing and activation of heparin-binding EGF-like growth factor (HB-EGF), stimulating the EGFR signaling pathway to increase proliferation and tumor growth [23], which was related with cervical cancer [44,45].…”

Section: Discussionmentioning

confidence: 99%

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Long non-coding RNA CCAT1 promotes cervical cancer cell proliferation and invasion by regulating the miR-181a-5p/MMP14 axis

et al. 2019

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Cervical cancer is a serious threat to women's health and is the third most common malignancy in women worldwide. Recent studies indicate that the long non-coding RNA CCAT1 plays a role in the malignant behavior of many tumors. However, the role of CCAT1 in cervical cancer is still unknown. Our aim is to evaluate the expression and investigate the regulatory role and potential mechanism of CCAT1 in cervical cancer. CCAT1 expression was measured by qRT-PCR. In addition, CCK-8 assays, colony formation assays, qRT-PCR assays, Transwell assays and xenograft experiments were performed to determine the role of CCAT1 in the proliferation and invasion in cervical cancer cells. The expression of CCAT1 in the cervical cancer tissues was higher than in the adjacent normal tissues. Overexpressing CCAT1 promoted cervical cancer cell proliferation, colony formation, and invasion in vitro. Elevated CCAT1 suppressed miR-181a expression, which was accompanied by an increased expression of MMP14 and HB-EGF. In contrast, knocking down CCAT1 resulted in increased expression of miR-181a, along with decreased expression of MMP14 and HB-EGF. Thus, CCAT1 is a key oncogenic lncRNA associated with cervical cancer and plays a role in promoting cervical cancer cell proliferation and invasion by regulating the miR-181a-5p/MMP14 axis.

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Section: Ccat1 Regulates the Expression Of Mir-181a-5p And Matrix Metsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Long non-coding RNA CCAT1 promotes cervical cancer cell proliferation and invasion by regulating the miR-181a-5p/MMP14 axis

et al. 2019

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“…MMP14 positive expression is considered to connect with a high recurrence of CC patients [10]. And MMP14 down-regulation was found to impair the proliferation and invasion ability of CC cells [26]. Correspondingly, the CCK-8 assay and transwell assay in our study indicated that MMP14 inhibition by miR-195-5p led to a poor proliferation and invasion profile of CC cells.…”

Section: Cellular Physiology and Biochemistry Cellular Physiology Andmentioning

confidence: 71%

The Effects of miR-195-5p/MMP14 on Proliferation and Invasion of Cervical Carcinoma Cells Through TNF Signaling Pathway Based on Bioinformatics Analysis of Microarray Profiling

Ren

Zhang

et al. 2018

Cell Physiol Biochem

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Background/Aims: This study is aimed at identification of miR-195-5p/MMP14 expression in cervical cancer (CC) and their roles on cell proliferation and invasion profile of CC cells through TNF signaling pathway in CC. Methods: Microarray analysis, gene set enrichment analysis (GSEA) and DAVID were used to analyze differentially expressed miRNAs, mRNAs and signaling pathways. MiR-195-5p and MMP14 expression levels in CC cell were determined by qRT-PCR. Western blot was employed to measure MMP14 and TNF signaling pathway-relating protein level. Luciferase reporter system was used to confirm the targeting relationship between MMP14 and miR-195-5p. Cell proliferation and invasion was respectively deeded by CCK8, transwell. In vivo experiment was carried out to study the impact of MMP14 and miR-195-5p on CC development in mice. Results: The microarray analysis and the results of qRT-PCR determined that miR-195-5p was under-expressed and MMP14 was over-expressed in CC cells. GSEA and DAVID analysis showed that TNF signaling pathway was regulated by miR-195-5p/MMP14 and activated in cervical carcinoma cells. The miR-195-5p and MMP14 have a negative regulation relation. In vivo experiment found that down-regulated MMP14 and up-regulated miR-195-5p suppressed the tumor development. Conclusion: Our results suggest that MMP14 is a direct target of miR-195-5p, and down-regulated MMP14 and up-regulated miR-195-5p suppressed proliferation and invasion of CC cells by inhibiting TNF signaling pathway.

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SPP1 inhibition improves the cisplatin chemo-sensitivity of cervical cancer cell lines

Xing

Xiong

et al. 2019

Cancer Chemother Pharmacol

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Matrix Metallopeptidase 14 Plays an Important Role in Regulating Tumorigenic Gene Expression and Invasion Ability of HeLa Cells

Abstract: Matrix metallopeptidase 14 plays an important role in regulating invasion of HeLa cells. Matrix metallopeptidase 14 knockdown contributes to attenuating the malignant phenotype of cervical cancer cell.

Cited by 4 publications

References 31 publications

Long non-coding RNA CCAT1 promotes cervical cancer cell proliferation and invasion by regulating the miR-181a-5p/MMP14 axis

Long non-coding RNA CCAT1 promotes cervical cancer cell proliferation and invasion by regulating the miR-181a-5p/MMP14 axis

The Effects of miR-195-5p/MMP14 on Proliferation and Invasion of Cervical Carcinoma Cells Through TNF Signaling Pathway Based on Bioinformatics Analysis of Microarray Profiling

SPP1 inhibition improves the cisplatin chemo-sensitivity of cervical cancer cell lines

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