Matrix-localization for fast analysis of arrayed microfluidic immunoassays

Zhang, Yi; Wang, Xuwei; Song, Lusheng; Xu, Chuanlai; Ma, Liying; Li, Zhanhua; Xi, Jianzhong; Jiang, Xingyu

doi:10.1039/c2ay25485a

Cited by 11 publications

(7 citation statements)

References 23 publications

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“…It only requires the dimension of the microfluidic channels to be regular (a constant width and a constant spacing), which is convenient for interrogating the size information. 29 This limitation does not affect the throughput of assays, and we usually use the chip with fixed channel width and fixed channel spacing. The advantage of our approach will be much more clear with the increase of the throughput.…”

Section: Further Discussionmentioning

confidence: 99%

“…Our own work and many works by other groups in the arrayed immunoassay have shown the wide applicability to disease biomarkers, small molecules, cell surface receptors, and so forth. 29,[44][45][46] It is straightforward to combine our proposed 2D barcode principle with these systems to greatly enhance the overall efficiency. Because the function patterns and the arrayed spots are relatively independent of each other, the modification to the conventional immunoassay does not affect the analytical performance of such system.…”

Section: Further Discussionmentioning

confidence: 99%

“…29 We transferred the relief structure to the PDMS by replica molding. We assembled the PDMS channels with a PDMS slab to generate the sealed channels which guide the liquid flow.…”

Section: Device Fabricationmentioning

confidence: 99%

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Two dimensional barcode-inspired automatic analysis for arrayed microfluidic immunoassays

et al. 2013

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The usability of many high-throughput lab-on-a-chip devices in point-of-care applications is currently limited by the manual data acquisition and analysis process, which are labor intensive and time consuming. Based on our original design in the biochemical reactions, we proposed here a universal approach to perform automatic, fast, and robust analysis for high-throughput array-based microfluidic immunoassays. Inspired by two-dimensional (2D) barcodes, we incorporated asymmetric function patterns into a microfluidic array. These function patterns provide quantitative information on the characteristic dimensions of the microfluidic array, as well as mark its orientation and origin of coordinates. We used a computer program to perform automatic analysis for a high-throughput antigen/antibody interaction experiment in 10 s, which was more than 500 times faster than conventional manual processing. Our method is broadly applicable to many other microchannel-based immunoassays. V C 2013 AIP Publishing LLC. [http://dx

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Section: Further Discussionmentioning

confidence: 99%

Section: Further Discussionmentioning

confidence: 99%

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Two dimensional barcode-inspired automatic analysis for arrayed microfluidic immunoassays

et al. 2013

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“…To tackle this problem, our group present a programming-based image processing method by analyzing signals in identified sub-array reaction zone (Figure 2a). [34] Using this method, the image analysis can be finished in 5 minutes. However, in order to acquire data from the bioassays, the position of the region of interest (ROI) had to be manually defined.…”

Section: Reviewmentioning

confidence: 99%

Materials for Microfluidic Immunoassays: A Review

Mou

Jiang

2017

Adv Healthcare Materials

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115

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Conventional immunoassays suffer from at least one of these following limitations: long processing time, high costs, poor user‐friendliness, technical complexity, poor sensitivity and specificity. Microfluidics, a technology characterized by the engineered manipulation of fluids in channels with characteristic lengthscale of tens of micrometers, has shown considerable promise for improving immunoassays that could overcome these limitations in medical diagnostics and biology research. The combination of microfluidics and immunoassay can detect biomarkers with faster assay time, reduced volumes of reagents, lower power requirements, and higher levels of integration and automation compared to traditional approaches. This review focuses on the materials‐related aspects of the recent advances in microfluidics‐based immunoassays for point‐of‐care (POC) diagnostics of biomarkers. We compare the materials for microfluidic chips fabrication in five aspects: fabrication, integration, function, modification and cost, and describe their advantages and drawbacks. In addition, we review materials for modifying antibodies to improve the performance of the reaction of immunoassay. We also review the state of the art in microfluidic immunoassays POC platforms, from the laboratory to routine clinical practice, and also commercial products in the market. Finally, we discuss the current challenges and future developments in microfluidic immunoassays.

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“…Our design is inspired by microfluidic micromosaic immunoassay, [20][21][22] which involves perpendicularly introducing a set of different antigens and a set of antibodies in microfluidic channels. By using parallel microfluidic channels perpendicular to the antigen bands to introduce varying antibodies, we could realize multiplexed blotting.…”

Section: Device Design and Assemblymentioning

confidence: 99%

Multiplexed microfluidic blotting of proteins and nucleic acids by parallel, serpentine microchannels

Zhang

Wang

et al. 2015

Lab Chip

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This work develops a high-throughput, high-efficiency and straightforward microfluidic blotting method for analyzing proteins and nucleic acids. Sample solutions containing antibodies (for protein detection) or hybridization probes (for nucleic acid detection) are introduced into the parallel, serpentine microchannels to specifically recognize the immobilized targets on the substrate, achieving the identification of multiple targets in multiple samples simultaneously. The loading control, molecular weight markers, and antigen/antibody titration are designed and integrated into the microfluidic chip, thus allowing for the quantification of proteins and nucleic acids. Importantly, we could easily distinguish the adjacent blotting bands inside parallel microchannels, which may be difficult to achieve in conventional blotting. The small dimensions of microfluidic channels also help to reduce the amount of probing molecules and to accelerate the biochemical reaction. Our microfluidic blotting could bypass the steps of blocking and washing, further reducing the operation time and complexity.

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Matrix-localization for fast analysis of arrayed microfluidic immunoassays

Cited by 11 publications

References 23 publications

Two dimensional barcode-inspired automatic analysis for arrayed microfluidic immunoassays

Two dimensional barcode-inspired automatic analysis for arrayed microfluidic immunoassays

Materials for Microfluidic Immunoassays: A Review

Multiplexed microfluidic blotting of proteins and nucleic acids by parallel, serpentine microchannels

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