Matrix-Induced Autologous Chondrocyte Implantation

Gibson, AJ; McDonnell, Susan; Price, Andrew

doi:10.1053/j.oto.2006.09.004

Cited by 8 publications

(7 citation statements)

References 20 publications

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“…As articular cartilage is avascular and has a limited intrinsic healing capacity, even small defects of articular cartilage can deteriorate and lead to osteoarthritis 1,2 . Current clinical treatments for repairing cartilage defects include autologous chondrocyte implantation (ACI) 3 , matrix-induced autologous chondrocyte implantation (MACI) 4 , micro fracture 5 , and allografts 6 . However, each of these treatments have had limited success over the long-term.…”

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confidence: 99%

Mechanical confinement regulates cartilage matrix formation by chondrocytes

et al. 2017

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Cartilage tissue equivalents formed from hydrogels containing chondrocytes could provide a solution for replacing damaged cartilage. Previous approaches have often utilized elastic hydrogels. However, elastic stresses may restrict cartilage matrix formation and alter the chondrocyte phenotype. Here we investigated the use of viscoelastic hydrogels, in which stresses are relaxed over time and which exhibit creep, for 3D culture of chondrocytes. We found that faster relaxation promoted a striking increase in the volume of interconnected cartilage matrix formed by chondrocytes. In slower relaxing gels, restriction of cell volume expansion by elastic stresses led to increased secretion of IL-1β, which in turn drove strong up-regulation of genes associated with cartilage degradation and cell death. As no cell adhesion ligands are presented by the hydrogels, these results reveal cell sensing of cell volume confinement as an adhesion-independent mechanism of mechanotransduction in 3D culture, and highlight stress relaxation as a key design parameter for cartilage tissue engineering.

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confidence: 99%

Mechanical confinement regulates cartilage matrix formation by chondrocytes

et al. 2017

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“…We expect that the mechanical stiffness, which is 13 times higher than the tested standard biomaterials currently in use for cartilage regeneration in clinics, has the potential to protect the neocartilage formation from overload and trauma [ 1 , 77 ]. Therefore it might tolerate loading early after surgery and shorten the postoperative physiotherapy [78] , [79] , [80] , [81] .…”

Section: Discussionmentioning

confidence: 99%

Repopulation of decellularised articular cartilage by laser-based matrix engraving

et al. 2021

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Background In spite of advances in the treatment of cartilage defects using cell and scaffold-based therapeutic strategies, the long-term outcome is still not satisfying since clinical scores decline years after treatment. Scaffold materials currently used in clinical settings have shown limitations in providing suitable biomechanical properties and an authentic and protective environment for regenerative cells. To tackle this problem, we developed a scaffold material based on decellularised human articular cartilage. Methods Human articular cartilage matrix was engraved using a CO 2 laser and treated for decellularisation and glycosaminoglycan removal. Characterisation of the resulting scaffold was performed via mechanical testing, DNA and GAG quantification and in vitro cultivation with adipose-derived stromal cells (ASC). Cell vitality, adhesion and chondrogenic differentiation were assessed. An ectopic, unloaded mouse model was used for the assessment of the in vivo performance of the scaffold in combination with ASC and human as well as bovine chondrocytes. The novel scaffold was compared to a commercial collagen type I/III scaffold. Findings Crossed line engravings of the matrix allowed for a most regular and ubiquitous distribution of cells and chemical as well as enzymatic matrix treatment was performed to increase cell adhesion. The biomechanical characteristics of this novel scaffold that we term CartiScaff were found to be superior to those of commercially available materials. Neo-tissue was integrated excellently into the scaffold matrix and new collagen fibres were guided by the laser incisions towards a vertical alignment, a typical feature of native cartilage important for nutrition and biomechanics. In an ectopic, unloaded in vivo model, chondrocytes and mesenchymal stromal cells differentiated within the incisions despite the lack of growth factors and load, indicating a strong chondrogenic microenvironment within the scaffold incisions. Cells, most noticeably bone marrow-derived cells, were able to repopulate the empty chondrocyte lacunae inside the scaffold matrix. Interpretation Due to the better load-bearing, its chondrogenic effect and the ability to guide matrix-deposition, CartiScaff is a promising biomaterial to accelerate rehabilitation and to improve long term clinical success of cartilage defect treatment. Funding Austrian Research Promotion Agency FFG (“CartiScaff” #842455), Lorenz Böhler Fonds (16/13), City of Vienna Competence Team Project Signaltissue (MA23, #18-08)

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“…Matrix-induced ACI (M-ACI) is a third-generation technique that was developed to utilise tissue engineering to provide a scaffold and even cell distribution in the final graft. In this technique, cultured cells are seeded onto a collagen membrane matrix in the laboratory 74. In contrast, both P-ACI and M-ACI require the chondrocyte suspension to be injected under a membrane at the time of surgery, which can cause uneven distribution in the lesion due to the effects of gravity 74.…”

Section: Articular Cartilage Surgical Treatmentmentioning

confidence: 99%

“…In this technique, cultured cells are seeded onto a collagen membrane matrix in the laboratory 74. In contrast, both P-ACI and M-ACI require the chondrocyte suspension to be injected under a membrane at the time of surgery, which can cause uneven distribution in the lesion due to the effects of gravity 74. M-ACI is technically attractive because it is a simplified surgical procedure that can be performed with a limited open approach or even arthroscopically, thereby avoiding the morbidity and delayed rehabilitation associated with an arthrotomy.…”

Section: Articular Cartilage Surgical Treatmentmentioning

confidence: 99%