Matrix effect marker for multianalyte analysis by LC–MS/MS in biological samples

Tudela, Eva; Muñoz, Gonzalo; Muñoz‐Guerra, Jesús

doi:10.1016/j.jchromb.2012.06.007

Cited by 27 publications

(14 citation statements)

References 19 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Obviously and because of the limitations (sample volume) and ethical constrains in neonates, such studies should first be done using samples for adult patients. Matrix effects (ion suppression/enhancement) should hereby be considered, since this is a well-observed phenomenon in analyses of biological matrices by high-performance liquid chromatography-mass spectrometry (LC-MS) [13]. This should be followed by subsequent hypothesis testing, validation, or confirmation in neonatal matrix.…”

Section: Resultsmentioning

confidence: 99%

Different Vancomycin Immunoassays Contribute to the Variability in Vancomycin Trough Measurements in Neonates

Samardžić

Smits

Spriet

et al. 2016

BioMed Research International

View full text Add to dashboard Cite

Substantial interassay variability (up to 20%) has been described for vancomycin immunoassays in adults, but the impact of neonatal matrix is difficult to quantify because of blood volume constraints in neonates. However, we provide circumstantial evidence for a similar extent of variability. Using the same vancomycin dosing regimens and confirming similarity in clinical characteristics, vancomycin trough concentrations measured by PETINIA (2011-2012, n = 400) were 20% lower and the mean difference was 1.93 mg/L compared to COBAS (2012–2014, n = 352) measurements. The impact of vancomycin immunoassays in neonatal matrix was hereby suggested, supporting a switch to more advanced techniques (LC-MS/MS).

show abstract

Section: Resultsmentioning

confidence: 99%

Different Vancomycin Immunoassays Contribute to the Variability in Vancomycin Trough Measurements in Neonates

Samardžić

Smits

Spriet

et al. 2016

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…However, the proportion of urine specimens containing a large number of leukocytes was much higher among the specimens correctly identified by Vitek MS, suggesting that a high leukocyte count does not interfere with bacterial protein peaks. High concentrates of many endogenous substances such as α-defensins in urine, which can suppress or enhance the ionization of certain protein spectra of microorganisms, might result in incorrect or no identification [ 13 , 14 ].…”

Section: Discussionmentioning

confidence: 99%

Direct Identification of Urinary Tract Pathogens From Urine Samples Using the Vitek MS System Based on Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

et al. 2015

View full text Add to dashboard Cite

BackgroundWe evaluated the coincidence rate between Vitek MS system (bioMérieux, France) and Vitek 2 in identifying uropathogens directly from urine specimens.MethodsUrine specimens submitted to our microbiology laboratory between July and September 2013 for Gram staining and bacterial culture were analyzed. Bacterial identification was performed by using the conventional method. Urine specimens showing a single morphotype by Gram staining were processed by culturing and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Of 2,370 urine specimens, 251 showed a single morphotype on Gram staining, and among them, 202 were available for MALDI-TOF MS.ResultsIn these 202 specimens, colony growth was observed in 189 specimens, and 145 specimens had significant growth of single-colony morphotype in culture. One hundred and ten (75.9%) of them had colony counts of ≥105 colony-forming units (CFU)/mL and included 71 enteric gram-negative bacteria (GNB), 5 glucose-non-fermenting GNB, 9 gram-positive cocci (GPC), and 25 yeasts. Furthermore, 70 (98.6%), 3 (60.0%), 4 (44.4%), and 5 (20.0%), respectively, of these were correctly identified by Vitek MS. Thirty-one specimens (21.4%; 11 GNB, 7 GPC, 12 yeasts, and 1 gram-positive bacillus) had colony counts of 104-105 CFU/mL. Four specimens (2.8%) yielded colony counts of 103-104 CFU/mL.ConclusionsVitek MS showed high rate of accuracy for the identification of GNB in urine specimens (≥105 CFU/mL). This could become a rapid and accurate diagnostic method for urinary tract infection caused by GNB. However, for the identification of GPC and yeasts, further studies on appropriate pre-treatment are warranted.

show abstract

“…Matrix effects are common in urine analyses which are already observed as interferences in the calibration straight lines (Tudela et al, 2012;Deventer et al, 2014). Human urine samples diluted in water are generally used for the preparation of these calibration straight lines (Olsson et al, 2004;Davis et al, 2013;Roca et al, 2014aRoca et al, , 2014b.…”

Section: Matrix Effectsmentioning

confidence: 99%

Analysis of metabolites of organophosphate and pyrethroid pesticides in human urine from urban and agricultural populations (Catalonia and Galicia)

Garí

González-Quinteiro

Bravo

et al. 2018

Science of The Total Environment

View full text Add to dashboard Cite

Isotope dilution solid phase extraction UPLC-MS/MS has been used to develop a robust and rapid methodology for the determination of eight specific metabolites of organophosphate and pyrethroid pesticides in human urine. The use of methanol:acetone (25:75v/v) affords an improvement in extraction efficiency in comparison to these individual solvents. The use of synthetic urine improves selectivity and limits of detection for the calibration straight lines. The method provides detection limits of 14-69pg/ml and 18-19pg/ml for the organophosphate and pyrethroid metabolites, respectively. Urine analyses of these metabolites in urban non-occupationally exposed individuals and farm workers shows that ingestion of these pesticides occurred in both populations. The concentrations of organophosphate pesticide metabolites in the latter were twofold than those from non-exposed populations.

show abstract

Matrix effect marker for multianalyte analysis by LC–MS/MS in biological samples

Cited by 27 publications

References 19 publications

Different Vancomycin Immunoassays Contribute to the Variability in Vancomycin Trough Measurements in Neonates

Different Vancomycin Immunoassays Contribute to the Variability in Vancomycin Trough Measurements in Neonates

Direct Identification of Urinary Tract Pathogens From Urine Samples Using the Vitek MS System Based on Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Analysis of metabolites of organophosphate and pyrethroid pesticides in human urine from urban and agricultural populations (Catalonia and Galicia)

Contact Info

Product

Resources

About