Matrix-compatible solid phase microextraction coating improves quantitative analysis of volatile profile throughout brewing stages

Hernandes, Karolina Cardoso; Souza-Silva, Érica A.; Assumpção, Carolina Fagundes; Zini, Cláudia Alcaraz; Welke, Juliane Elisa

doi:10.1016/j.foodres.2019.04.048

Cited by 13 publications

(2 citation statements)

References 30 publications

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“…Several calibration curves for the targeted analytes demonstrated a lack of linearity, and the quantitative evaluation was distorted due to coating saturation and competition between components. A similar displacement phenomenon was also reported in work attempting to quantitate the volatile profiles of the five stages of brewing, as well as volatile free fatty acids and phenols, volatile organic sulfur compounds, and volatile flavor compounds in coffee samples. − …”

supporting

confidence: 66%

Sequential Solid-Phase Microextraction with Biocompatible Coating Materials to Address Displacement Effects in the Quantitative Analysis

2023

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Solid-phase microextraction (SPME) is a simple and effective sample-preparation technique for the analysis of complex samples. However, sample matrices containing high concentrations of nonpolar substances or spiked analytes in free form can cause swelling, saturation, and/or competition phenomena in the coating material. This results in a displacement effect wherein polar analytes with low affinities for the solid coating material are displaced by nonpolar substances in the matrix or spiked analytes with a high affinity. Therefore, the quantitative analysis of polar analytes can be challenging, as the displacement effect causes non-linearity in the calibration curves. This paper presents a comprehensive investigation of the conditions under which the displacement effect occurs and how it influences the quantitative analysis of polar analytes. To remedy this issue, a sequential SPME strategy using two SPME blades with different selectivities is applied. SPME blades offer a large surface area and coating volume�and thus, greater extraction capacity�which may mitigate the displacement effect. In addition, the biocompatible coatings on the SPME blades are comprised of small amounts of sorbent particles embedded by a polyacrylonitrile (PAN) binder, which allows them to be directly immersed into complex matrixes such as biological and food samples, as the PAN acts as a barrier that prevents the adsorption of large macromolecules (e.g., cells and proteins). As such, a C18/PAN-coated blade was applied for the first extraction step, which significantly decreased the concentrations of nonpolar compounds in the sample. In the second step, a hydrophilic−lipophilic balanced (HLB)/PAN-coated blade was employed to extract the polar analytes and any remaining nonpolar analytes. The proposed sequential SPME strategy successfully enabled the quantitative determination of polar and nonpolar drugs of abuse with log P values ranging from 0.16 to 4.98 in biological matrices while also providing good linearities.

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supporting

confidence: 66%

Sequential Solid-Phase Microextraction with Biocompatible Coating Materials to Address Displacement Effects in the Quantitative Analysis

2023

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“…Because HS-SPME is an equilibrium process, when HS-SPME-GC×GC-TOFMS is used to quantify trace compounds, a problem of competitive adsorption exists. Ethanol is the major matrix constituent of Baijiu and has been reported as an important interfering volatile during HS-SPME of trace compounds [27], especially for the hydrophobic analytes, which suffered more strongly from the competition between the aqueous alcoholic solution and the fiber coating [28]. The selection of an appropriate dilution ratio may reduce matrix interferences [29], so we reduced the effect of ethanol in quantitative analysis via sample dilution.…”

Section: Effect Of Sample Dilutionmentioning

confidence: 99%

Optimization and Validation of a Headspace Solid-Phase Microextraction with Comprehensive Two-Dimensional Gas Chromatography Time-of-Flight Mass Spectrometric Detection for Quantification of Trace Aroma Compounds in Chinese Liquor (Baijiu)

Gao

et al. 2021

Molecules

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The detection of trace aroma compounds in samples with complex matrices such as Chinese liquor (Baijiu) requires a combination of several methods, which makes the analysis process very complicated. Therefore, a headspace solid-phase microextraction (HS-SPME) method coupled with two-dimensional gas chromatography time-of-flight mass spectrometry (GC×GC-TOFMS) was developed for the quantitation of a large number of trace compounds in Baijiu. Optimization of extraction conditions via a series of experiments revealed that dilution of the alcohol content of 8 mL of Baijiu to 5%, followed by the addition of 3.0 g of NaCl and subsequent SPME extraction with DVB/CAR/PDMS fiber coating over 45 min at 45 °C was the most suitable. To check the matrix effects, various model Baijiu matrices were investigated in detail. The quantitative method was established through an optimized model synthetic solution, which can identify 119 aroma compounds (esters, alcohols, fatty acids, aldehydes and ketones, furans, pyrazines, sulfur compounds, phenols, terpenes, and lactones) in the Baijiu sample. The developed procedure provided high recovery (86.79–117.94%), good repeatability (relative standard deviation < 9.93%), high linearity (R2 > 0.99), and lower detection limits than reported methods. The method was successfully applied to study the composition of volatile compounds in different types of Baijiu. This research indicated that the optimized HS-SPME–GC×GC-TOFMS method was a valid and accurate procedure for the simultaneous determination of different types of trace compounds in Baijiu. This developed method will allow an improved analysis of other samples with complex matrices.

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Modulated construction of imine-based covalent organic frameworks for efficient adsorption of polycyclic aromatic hydrocarbons from honey samples

Yang

Zhang

Wang

et al. 2020

Analytica Chimica Acta

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Matrix-compatible solid phase microextraction coating improves quantitative analysis of volatile profile throughout brewing stages

Cited by 13 publications

References 30 publications

Sequential Solid-Phase Microextraction with Biocompatible Coating Materials to Address Displacement Effects in the Quantitative Analysis

Sequential Solid-Phase Microextraction with Biocompatible Coating Materials to Address Displacement Effects in the Quantitative Analysis

Optimization and Validation of a Headspace Solid-Phase Microextraction with Comprehensive Two-Dimensional Gas Chromatography Time-of-Flight Mass Spectrometric Detection for Quantification of Trace Aroma Compounds in Chinese Liquor (Baijiu)

Modulated construction of imine-based covalent organic frameworks for efficient adsorption of polycyclic aromatic hydrocarbons from honey samples

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