Matrix-based autologous chondrocyte implantation for cartilage repair: noninvasive monitoring by high-resolution magnetic resonance imaging

Trattnig, Siegfried; Ba‐Ssalamah, Ahmed; Pinker, Katja; Plank, Christina; Vécsei, V.; Marlovits, Stefan

doi:10.1016/j.mri.2005.04.010

Cited by 131 publications

(117 citation statements)

References 29 publications

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“…Also, the use of a charged contrast agent such as gadolinium allows direct visualisation of glycosaminoglycan content in cartilage (Allen et al, 1999). Both these techniques have been used to study the process of cartilage repair non-invasively over long post-operative periods using ACI (Roberts et al, 2003) and MACI (Trattnig et al, 2005(Trattnig et al, , 2006, where they have been most useful in increasing our understanding of the maturation processes that occur during healing, especially with respect to lateral integration. One study, covering the postoperative repair process over 52 weeks, describes the MRI signal from a cell-seeded HyalograftC™ graft transitioning from a fluid-like signal, to hypo-intensity, and finally isointensity when compared to the surrounding articular cartilage (Trattnig et al, 2006).…”

Section: Cartilage Integrationmentioning

confidence: 99%

Evaluation of the reasons for failure of integration during cartilage repair. A review

Khan¹,

Gilbert²,

Singhrao³

et al. 2008

eCM

236

221

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Articular cartilage is a challenging tissue to reconstruct or replace principally because of its avascular nature; large chondral lesions in the tissue do not spontaneously heal. Where lesions do penetrate the bony subchondral plate, formation of hematomas and the migration of mesenchymal stem cells provide an inferior and transient fibrocartilagenous replacement for hyaline cartilage. To circumvent the poor intrinsic reparative response of articular cartilage several surgical techniques based on tissue transplantation have emerged. One characteristic shared by intrinsic reparative processes and the new surgical therapies is an apparent lack of lateral integration of repair or graft tissue with the host cartilage that can lead to poor prognosis. Many factors have been cited as impeding cartilage:cartilage integration including; chondrocyte cell death, chondrocyte dedifferentiation, the nature of the collagenous and proteoglycan networks that constitute the extracellular matrix, the type of biomaterial scaffold employed in repair and the origin of the cells used to repopulate the defect or lesion. This review addresses the principal intrinsic and extrinsic factors that impede integration and describe how manipulation of these factors using a host of strategies can positively influence cartilage integration. Keywords IntroductionArticular cartilage is a highly organised avascular and aneural tissue that provides a smooth surface for the movement of articulating bones and transmission of loads (Muir, 1995). Cartilage carries out the latter function by transferring the forces generated by locomotion to the underlying bone. The resident cells of articular cartilage are chondrocytes that are surrounded by an extensive extracellular matrix whose primary constituents are water, aggrecans and type II collagen. Aggrecans are rich in covalently bonded glycosaminoglycans that are hydrophilic and whose electronegative properties resist applied compressive forces. The tensile strength required to constrain the electronegative force generated by the aggrecans is provided by an organised network of crosslinked fibrils principally containing type II collagen. In cross-section articular cartilage displays a pseudostratified appearance composed of three unmineralised layers; the superficial zone with small, dicoidal chondrocytes aligned parallel to the surface, a transitional zone where chondrocytes are rounded with no apparent organisation and the radial/deep zone where large chondrocytes are aligned in columns of 4-6 cells at right-angles to the surface, Figure 1. The fourth layer is the calcified zone where mineralisation is restricted to the interterritorial matrix of chondrocytes. The calcified zone borders and interdigitates with the subchondral bone. Cartilage defects TraumaIn younger patients who have generally suffered trauma to a joint, focal lesions may appear that if untreated may lead to further progressive degeneration over time. Clinically, focal lesions are graded from the appearance of superficial fissure...

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Section: Cartilage Integrationmentioning

confidence: 99%

Evaluation of the reasons for failure of integration during cartilage repair. A review

Khan¹,

Gilbert²,

Singhrao³

et al. 2008

eCM

236

221

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“…Transplantation of autologous or allogenous chondrocytes, synovial mesenchymal stem cells, and biodegradable scaffolds are the methods that have been tested experimentally and clinically [5][6][7][8][9][10][11]. Implants seeded with chondrocytes cultured in vitro support the production of the extracellular matrix by chondrocytes, mainly glycosaminoglycans and type II collagen [12,13]. The implantation of chondrocyte-collagen composites into cartilage defects has proved a promising method of cartilage repair [14,15] and numerous in vivo studies in animals have shown that hyaluronan-based scaffolds seeded with autologous chondrocytes are useful for inducing the formation of hyaline-like cartilage tissue and are reabsorbed in the absence of an infl ammatory response [16,17].…”

Section: Research Articlementioning

confidence: 99%

Reparation of chondral defects in rabbits by autologous and allogenous chondrocytes seeded on collagen/hyaluronan scaffold or suspended in fibrin glue

Horňák¹,

Harvanová²,

Ledecký³

et al. 2014

Acta Veterinaria

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The topic of the study was to verify in vivo survival of in vitro cultured autologous and allogenous chondrocytes suspended in a fibrin glue Beriplast® or seeded on Collagen type I-Hyaluronan (Col type I-HYA) scaffolds for the regeneration of articular cartilage defects in rabbits. The study was carried out on 15 domestic rabbits randomly assigned to five groups (n = 3 in each) with different treatments of artificially created chondral defects (ChD´s). These defects were made in a non-load-bearing area of medial condyle of the distal femur, and were treated as follows: 1st and 3rd group: the ChD´s were filled with autologous or allogenous chondrocytes seeded on Col type I-HYA scaffolds, respectively. The scaffolds were fixed to the ChD´s by fibrin glue Beriplast®; 2nd and 4th group: the ChD´s were filled with a suspension of autologous or allogenous chondrocytes in fibrin glue Beriplast®, respectively, and they were immediately covered by unseeded Col type I-HYA scaffolds; Control group: the ChD´s were left to heal spontaneously without any treatment. Macroscopical, histological and immunohistochemical analyses of the ChD´s were performed 12 months after the treatment. In all treated groups, the chondrocytes were capable to proliferate and produce the cartilage extracellular matrix, including proteoglycans and type II collagen, as compared to the control “untreated” group. On the other hand, the production of hyaline-like cartilage tissue confirmed that both therapeutic methods using autologous chondrocytes can be applied successfully for the treatment of chondral defects in rabbits.

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“…Postoperative evaluation of patients receiving matrixinduced procedures using MRI have shown adequate quality of the tissue, with almost complete filling of the lesion, restoration of the articular surface and integration of the tissue (Cherubino et al, 2003;Trattnig et al, 2005). Second-look arthroscopies have shown incorporation of the graft into the knee and a macroscopic appearance similar to the surrounding healthy cartilage.…”

Section: Autologous Chondrocyte Transplantationmentioning

confidence: 99%

“…Brittberg et al (1994) initially reported the "first generation" of autologous chondrocyte implantation (ACI) in which a solution of in vitro cultured and expanded autologous chondrocytes were injected over the lesion under the periosteal fl ap. In the last 10 years this technique has been improved, using scaff olds as carriers for cells, in vitro cartilage-tissue constructions and using MSCs as a source to generate hyaline cartilage (Russlies et al, 2002;Trattnig et al, 2005). In addition, long-term follow-up studies reporting the outcomes of knee chondral lesions treated with the primary ACI and more recent techniques to transplant cells have been published, with adequate results (Beris et al, 2012;Filardo et al, 2012a;Pelissier et al, 2013;Rogers et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Clinical and experimental approaches to knee cartilage lesion repair and mesenchymal stem cell chondrocyte differentiation

Montoya¹,

Martínez²,

García-Robles³

et al. 2013

Biol. Res.

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Cartilage has poor regeneration capacity due to the scarcity of endogenous stem cells, its low metabolic activity and the avascular environment. Repair strategies vary widely, including microfracture, autologous or allogenic tissue implantation, and in vitro engineered tissues of autologous origin. However, unlike the advances that have been made over more than two decades with more complex organs, including vascular, cardiac or bone tissues, similar advances in tissue engineering for cartilage repair are lacking. Although the inherent characteristics of cartilage tissue, such as the lack of vascularity and low cellular diversity, suggest that it would be one of the more simple tissues to be engineered, its functional weight-bearing role and implant viability and adaptation make this type of repair more complex. Over the last decade several therapeutic approaches and innovative techniques show promise for lasting and functional regeneration of hyaline cartilage. Here we will analyze the main strategies for cartilage regeneration and discuss our experience.

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Matrix-based autologous chondrocyte implantation for cartilage repair: noninvasive monitoring by high-resolution magnetic resonance imaging

Cited by 131 publications

References 29 publications

Evaluation of the reasons for failure of integration during cartilage repair. A review

Evaluation of the reasons for failure of integration during cartilage repair. A review

Reparation of chondral defects in rabbits by autologous and allogenous chondrocytes seeded on collagen/hyaluronan scaffold or suspended in fibrin glue

Clinical and experimental approaches to knee cartilage lesion repair and mesenchymal stem cell chondrocyte differentiation

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