Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI–TOF–MS) coupled to XAD fractionation: Method to algal organic matter characterization

Nicolau, Rudy; Leloup, Maud; Lachassagne, D.; Pinault, Émilie; Feuillade-Cathalifaud, Geneviève

doi:10.1016/j.talanta.2015.01.011

Cited by 14 publications

(9 citation statements)

References 44 publications

(56 reference statements)

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“…The results were different during the early stationary phase: proteic markers were relatively more abundant in HPO than in TPH, contrary to the sterols ones [ 28 ]. These differences in composition may explain why transformation and stabilization processes were different for each fraction.…”

Section: Resultsmentioning

confidence: 99%

Assessing Transformations of Algal Organic Matter in the Long-Term: Impacts of Humification-Like Processes

Leloup

Pallier

Nicolau

et al. 2015

IJMS

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Algae and cyanobacteria are important contributors to the natural organic matter (NOM) of eutrophic water resources. The objective of this work is to increase knowledge on the modifications of algal organic matter (AOM) properties in the long term to anticipate blooms footprint in such aquatic environments. The production of AOM from an alga (Euglena gracilis) and a cyanobacteria (Microcystis aeruginosa) was followed up and characterized during the stationary phase and after one year and four months of cultivation, in batch experiments. Specific UV absorbance (SUVA) index, organic matter fractionation according to hydrophobicity and apparent molecular weight were combined to assess the evolution of AOM. A comparison between humic substances (HS) mainly derived from allochthonous origins and AOM characteristics was performed to hypothesize impacts of AOM transformation processes on the water quality of eutrophic water resources. Each AOM fraction underwent a specific evolution pattern, depending on its composition. Impacts of humification-like processes were predominant over release of biopolymers due to cells decay and led to an increase in the hydrophobic compounds part and molecular weights over time. However, the hydrophilic fraction remained the major fraction whatever the growth stage. Organic compounds generated by maturation of these precursors corresponded to large and aliphatic structures.

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Section: Resultsmentioning

confidence: 99%

Assessing Transformations of Algal Organic Matter in the Long-Term: Impacts of Humification-Like Processes

Leloup

Pallier

Nicolau

et al. 2015

IJMS

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“…Some advanced chemical analysis methods have been used to detect and identify cyanobacterial toxins accurately and sensitively [293], such as NMR, HPLC, CE, LC-MS, GC, etc. Another technique is matrix-assisted laser desorption-ionization-time-of-flight mass spectrometry (MALDI-TOF-MS), which can distinguish toxic from non-toxic strains at the level of a single colony or filament, without prior solvent extraction [294,295]. Currently, MS-based methods are considered to be the best approach to unambiguously identify and quantify the different variants of cyanotoxins.…”

Section: Identification Of Cyanotoxinsmentioning

confidence: 99%

The Diversity of Cyanobacterial Toxins on Structural Characterization, Distribution and Identification: A Systematic Review

Liu

Yuan

et al. 2019

Toxins

125

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The widespread distribution of cyanobacteria in the aquatic environment is increasing the risk of water pollution caused by cyanotoxins, which poses a serious threat to human health. However, the structural characterization, distribution and identification techniques of cyanotoxins have not been comprehensively reviewed in previous studies. This paper aims to elaborate the existing information systematically on the diversity of cyanotoxins to identify valuable research avenues. According to the chemical structure, cyanotoxins are mainly classified into cyclic peptides, alkaloids, lipopeptides, nonprotein amino acids and lipoglycans. In terms of global distribution, the amount of cyanotoxins are unbalanced in different areas. The diversity of cyanotoxins is more obviously found in many developed countries than that in undeveloped countries. Moreover, the threat of cyanotoxins has promoted the development of identification and detection technology. Many emerging methods have been developed to detect cyanotoxins in the environment. This communication provides a comprehensive review of the diversity of cyanotoxins, and the detection and identification technology was discussed. This detailed information will be a valuable resource for identifying the various types of cyanotoxins which threaten the environment of different areas. The ability to accurately identify specific cyanotoxins is an obvious and essential aspect of cyanobacterial research.

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“…Henderson et al (2008) showed that for Chlorella vulgaris 60% of the AOM consisted of molecules > 30 kDa and contributed to 84% of the total charge of the culture. However, cyanobacteria like Microcystis aeruginosa produce AOM that is richer in proteins compared to eukaryotic microalgae (Nicolau et al, 2015). This means that the inhibition of alkaline flocculation will depend on the composition of the AOM.…”

Section: Influence Of Aom Size and Composition On Flocculationmentioning

confidence: 99%

“…The concentration of AOM in the culture medium gradually increases with culture age and can reach up to 60-70 mg L -1 C in photobioreactor systems with high biomass concentrations, corresponding to 17% of the total fixed carbon (Hulatt and Thomas, 2010). AOM is composed of a wide range of compounds such as neutral and charged polysaccharides, proteins, nucleic acids, lipids, and small molecules, and has been shown to differ between species and cultivation conditions (Henderson et al, 2008;Nicolau et al, 2015;Villacorte et al, 2015). AOM interferes with flocculation and its presence results in a strong increase in flocculant demand (Chen et al, 2008;Vandamme et al, 2012b).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of alkaline flocculation by algal organic matter for Chlorella vulgaris

et al. 2016

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Alkaline flocculation is a promising strategy for the concentration of microalgae for bulk biomass production. However, previous studies have shown that biological changes during the cultivation negatively affect flocculation efficiency. The influence of changes in cell properties and in the quality and composition of algal organic matter (AOM) were studied using Chlorella vulgaris as a model species. In batch cultivation, flocculation was increasingly inhibited over time and mainly influenced by changes in medium composition, rather than biological changes at the cell surface. Total carbohydrate content of the organic matter fraction sized bigger than 3 kDa increased over time and this fraction was shown to be mainly responsible for the inhibition of alkaline flocculation. The monosaccharide identification of this fraction mainly showed the presence of neutral and anionic monosaccharides. The addition of 30-50 mg L(-1) alginic acid, as a model for anionic carbohydrate polymers containing uronic acids, resulted in a complete inhibition of flocculation. These results suggest that inhibition of alkaline flocculation was caused by interaction of anionic polysaccharides leading to an increased flocculant demand over time.

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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI–TOF–MS) coupled to XAD fractionation: Method to algal organic matter characterization

Cited by 14 publications

References 44 publications

Assessing Transformations of Algal Organic Matter in the Long-Term: Impacts of Humification-Like Processes

Assessing Transformations of Algal Organic Matter in the Long-Term: Impacts of Humification-Like Processes

The Diversity of Cyanobacterial Toxins on Structural Characterization, Distribution and Identification: A Systematic Review

Inhibition of alkaline flocculation by algal organic matter for Chlorella vulgaris

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