Matrix-assisted laser desorption/ionization–time of flight-mass spectrometry profiling of trace constituents of condom lubricants in the presence of biological fluids

Miko, Sandra E. Spencer; Kim, Sin Young; Kim, Seoung Bum; Schug, Kevin A.

doi:10.1016/j.forsciint.2010.08.010

Cited by 46 publications

(34 citation statements)

References 17 publications

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“…This class of agents can be applied directly on injured skin and ulcerated surfaces, where they remain localized for long periods of time to produce a prolonged anaesthetic action. Benzocaine is incorporated into a large number of topical preparations [11] for local anaesthesia, such as dental sprays, ear medication or in throat anaesthetic lozenges [13] and as a desensitizer in condoms [14]. …”

Section: Introductionmentioning

confidence: 99%

Thermal behaviour of procaine and benzocaine Part II: compatibility study with some pharmaceutical excipients used in solid dosage forms

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Ledeți

Vlase

et al. 2013

Chemistry Central Journal

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BackgroundThe compatibility study of active substances with excipients finds an important role in the domain of pharmaceutical research, being known the fact that final formulation is the one administered to the patient. In order to evaluate the compatibility between active substance and excipients, different analytical techniques can be used, based on their accuracy, reproducibility and fastness.ResultsCompatibility study of two well-known active substances, procaine and benzocaine, with four commonly used excipients, was carried out employing thermal analysis (TG/DTG/HF) and Fourier Transform Infrared Spectroscopy (UATR-FT-IR). The selected excipients were microcrystalline cellulose, lactose monohydrate, magnesium stearate and talc. Equal proportion of active substance and excipients (w/w) was utilized in the interaction study. The absolute value of the difference between the melting point peak of active substances and the one corresponding for the active substances in the analysed mixture, as well the absolute value of the difference between the enthalpy of the pure active ingredient melting peak and that of its melting peak in the different analysed mixtures were chosen as indexes of the drug-excipient interaction degree. All the results obtained through thermal analysis were also sustained by FT-IR spectroscopy.ConclusionsThe corroboration of data obtained by thermal analysis with the ones from FT-IR spectroscopy indicated that no interaction occurs between procaine and benzocaine, with microcrystalline cellulose and talc, as well for the benzocaine-lactose mixture. Interactions were confirmed between procaine and benzocaine respectively and magnesium stearate, and for procaine and lactose.

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Section: Introductionmentioning

confidence: 99%

Thermal behaviour of procaine and benzocaine Part II: compatibility study with some pharmaceutical excipients used in solid dosage forms

Fuliaş

Ledeți

Vlase

et al. 2013

Chemistry Central Journal

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“…Although nonoxynol-9 has been incorrectly presented as having an unbranched nonyl side chain [4,13], the chromatograms shown herein illustrate its inherent complexity. In total, both of the substituents branching off the core phenol structure of nonoxynol-9 are variable, making the overall "structure" an even more convoluted "mixture of mixtures," best described as the generic chemical structure in Figure 1.…”

Section: Resultsmentioning

confidence: 86%

“…Consequently, the most illustrative mass spectral data of nonoxynol-9 to date have been obtained by LC-MS and desorption chemical ionization MS, which yielded chromatograms in which the individual isomeric components were not resolved [4,5]. These techniques employ softer ionization methods that produce primarily molecular ions and simpler spectra with little to no fragmentation when compared with EI-based methods [6,[10][11][12][13]. The incorporation of a simple sonication step during the extraction was designed to disrupt micelles to better solubilize the material.…”

Section: Resultsmentioning

confidence: 99%

“…Significant effort has gone into developing advanced MS techniques for analysis of lubricants, in particular electrospray ionization-MS along with liquid chromatography-MS, matrix-assisted laser desorption ionization (MALDI)-MS, and other desorption techniques [4,6,[10][11][12][13]. However, these advanced methods and instrumentation are not widely utilized in crime laboratories.…”

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Detection of the Spermicide Nonoxynol-9 Via GC-MS

Musah

Vuong

Henck

et al. 2012

J. Am. Soc. Mass Spectrom.

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The spermicide nonoxynol-9 is actually a complex mixture of dozens of closely related amphiphilic compounds, and the chemical properties of this assortment significantly hamper its characterization by GC-MS. The inability to perform routine GC-MS testing on nonoxynol-9 has limited its evidentiary value in forensic casework, which relies heavily on this technique for analysis. A disturbing trend in sexual assault is the use of condoms by assailants, to avoid leaving behind DNA evidence that can connect a perpetrator to a victim. This observation necessitates the development of alternative methods for the analysis of trace evidence that can show causal links between a victim and a suspect. Detection of lubricants associated with sexual assault is one such way to establish this connection. The development of GC-MS methods that permit facile detection of both nonoxynol-9 alone and nonoxynol-9 extracted from other complex matrices that have potential as trace evidence in sexual assault is reported. A detection limit of 2.14 μg of nonoxynol-9 is demonstrated, and a detailed mass spectral profile that elaborates on what is known of its structure is provided.

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“…Extraction of the condom-related traces can be carried out with dichloromethane, followed by drying and re-solubilisation with methanol. Suitable matrices are 2,5-dihydroxybenzoic acid [123], dithranol in acetonitrile/dichloromethane/tetrahydrofuran (45:45:15 v/v) [124] and 2,5-dihydroxybenzoic acid and sodium chloride (75 mg/mL in acetone) [125]. Lubricants, additives and spermicides can be detected by this approach.…”

Section: Characterisation Of Other Additivesmentioning

confidence: 99%

Polymers on the Crime Scene

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2015

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Matrix-assisted laser desorption/ionization–time of flight-mass spectrometry profiling of trace constituents of condom lubricants in the presence of biological fluids

Cited by 46 publications

References 17 publications

Thermal behaviour of procaine and benzocaine Part II: compatibility study with some pharmaceutical excipients used in solid dosage forms

Thermal behaviour of procaine and benzocaine Part II: compatibility study with some pharmaceutical excipients used in solid dosage forms

Detection of the Spermicide Nonoxynol-9 Via GC-MS

Polymers on the Crime Scene

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