Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in clinical chemistry

Marvin, Laure; Roberts, Matthew; Fay, Laurent B.

doi:10.1016/j.cccn.2003.08.008

Cited by 202 publications

(119 citation statements)

References 77 publications

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“…Rapid and major developments in proteomics technology and methodology over the last decade have opened a new stage in the identification of proteins (9). MALDI-TOF-MS recently became available as a flexible tool in the search for disease markers (10). Moreover, the recently introduced technique of MALDI-FTMS provides a powerful technique for accurate peptide mass measurements (11).…”

mentioning

confidence: 99%

Identification of Glioma Neovascularization-related Proteins by Using MALDI-FTMS and Nano-LC Fractionation to Microdissected Tumor Vessels

Mustafa¹,

Burgers

Dekker

et al. 2007

Molecular & Cellular Proteomics

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The identification of angiogenesis-related proteins is important for the development of new antiangiogenic therapies, and such proteins are potential new biomarkers for gliomas. The aim of this study was to identify proteins that are exclusively present in glioma neovasculature and not in the vasculature of normal brain. We combined advanced proteomics techniques to compare the expression profiles of microdissected blood vessels from glioma with blood vessels of normal control brain samples. We measured the enzymatic generated peptide profiles from these microdissected samples by MALDI-FTMS. Subsequently, the samples were fractionated by nano-LC prior to MALDI-TOF/TOF. This combined approach enabled us to identify four proteins that appeared to be exclusively expressed in the glioma blood vessels. Two of these proteins, fibronectin and colligin 2, were validated on tissue sections using specific antibodies. We found that both proteins are present in active angiogenesis in glioma, other neoplasms, and reactive conditions in which neoangiogenesis takes place. This work proves that gel-free mass spectrometric techniques can be used on relatively

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mentioning

confidence: 99%

Identification of Glioma Neovascularization-related Proteins by Using MALDI-FTMS and Nano-LC Fractionation to Microdissected Tumor Vessels

Mustafa¹,

Burgers

Dekker

et al. 2007

Molecular & Cellular Proteomics

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“…It is a rapid method for bacterial and fungal identification, with high accuracy and low operating costs (23,26) . Fresh colonies (< 24h) are preferentially selected for performing bacterial identification by MALDI-TOF MS.…”

Section: A U T O M a T E D P H E N O T Y P I C T E S T S M A L D I -Tmentioning

confidence: 99%

“…These proteins are part of the cell translational apparatus and are present in all living cells. Therefore, MALDI-TOF MS is minimally influenced by environmental conditions or microorganism growth (23,26,29) .…”

Section: Maldi-tof Ms Analysismentioning

confidence: 99%

Evaluation of MALDI-TOF MS in the microbiology laboratory

Santos¹,

Cayô²,

Schandert³

et al. 2013

J. Bras. Patol. Med. Lab.

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Rapid identification of microorganisms by the clinical microbiology laboratory is of crucial importance for optimal patients' management and treatment. In general, bacterial identification by conventional methods requires 18-24 hours for colony isolation and at least 24 additional hours for species identification. New technologies in microbiology have focused on the rapid diagnosis of bloodstream infections, since they are associated with high morbidity and mortality rates.

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“…MALDI-TOF is one of the most widely used MS systems for proteomic studies (Henzel et al, 2003;Marvin et al, 2003;Ragoussis et al, 2006). This approach, although somewhat limited due to the analysis of relatively pure samples (for example, spots extracted from 2D gels), is highly advantageous due to its relatively low cost, simplicity in design and sensitivity.…”

Section: Principles Of Proteomicsmentioning

confidence: 99%

Application of proteomics to ecology and population biology

Karr

2007

Heredity

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Proteomics is a relatively new scientific discipline that merges protein biochemistry, genome biology and bioinformatics to determine the spatial and temporal expression of proteins in cells, tissues and whole organisms. There has been very little application of proteomics to the fields of behavioral genetics, evolution, ecology and population dynamics, and has only recently been effectively applied to the closely allied fields of molecular evolution and genetics. However, there exists considerable potential for proteomics to impact in areas related to functional ecology; this review will introduce the general concepts and methodologies that define the field of proteomics and compare and contrast the advantages and disadvantages with other methods. Examples of how proteomics can aid, complement and indeed extend the study of functional ecology will be discussed including the main tool of ecological studies, population genetics with an emphasis on metapopulation structure analysis. Because proteomic analyses provide a direct measure of gene expression, it obviates some of the limitations associated with other genomic approaches, such as microarray and EST analyses. Likewise, in conjunction with associated bioinformatics and molecular evolutionary tools, proteomics can provide the foundation of a systems-level integration approach that can enhance ecological studies. It can be envisioned that proteomics will provide important new information on issues specific to metapopulation biology and adaptive processes in nature. A specific example of the application of proteomics to sperm ageing is provided to illustrate the potential utility of the approach.

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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in clinical chemistry

Cited by 202 publications

References 77 publications

Identification of Glioma Neovascularization-related Proteins by Using MALDI-FTMS and Nano-LC Fractionation to Microdissected Tumor Vessels

Identification of Glioma Neovascularization-related Proteins by Using MALDI-FTMS and Nano-LC Fractionation to Microdissected Tumor Vessels

Evaluation of MALDI-TOF MS in the microbiology laboratory

Application of proteomics to ecology and population biology

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