Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry applied to virus identification

Calderaro, Adriana; Arcangeletti, Maria Cristina; Rodighiero, Isabella; Buttrini, Mirko; Gorrini, Chiara; Motta, Federica; Germini, Diego; Medici, Maria-Cristina; Chezzi, Carlo; Conto, Flora De

doi:10.1038/srep06803

Cited by 105 publications

(101 citation statements)

References 34 publications

(43 reference statements)

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“…On the contrary, in this study, echovirus type 30 and cytomegalovirus were added having been found them by cultivated clinical samples in a number of respiratory tract infections in our laboratory. MALDI-TOF MS is strictly dependent on the viral concentration in the sample and on the presence of molecules deriving from cell debris24. The present study demonstrated that with a minimum concentration of 10 6 TCID 50 /μl it is able to discriminate between virus infected and uninfected cell cultures.…”

Section: Discussionmentioning

confidence: 54%

“…The obtained results are unexpected, especially considering that a discrimination among adenovirus C types and parainfluenza types reference strains has been successful by using a discovery biomarkers software (ClinProTools), as also previously observed for poliovirus types24. This could be also a consequence of the fact that enveloped viruses have the capability of incorporating some host proteins packaged into their envelope29.…”

Section: Discussionmentioning

confidence: 66%

“…At present, mass spectrometry has had limited application in clinical virology6232425. In this light, the main purpose of this study was the development of a new tool based on MALDI-TOF MS as a model to be applied to the identification of cultivable respiratory viruses after a cell culture step.…”

Section: Discussionmentioning

confidence: 99%

“…Despite many literature data about MALDI-TOF MS application in routine clinical microbiology, and in experimental approaches related to bacteria, parasites, yeasts and moulds616171819202122, very few reports are available about its application in virus research and laboratory diagnosis of viral infections6232425. In most of the studies, specific viral genome sequences are previously amplified by PCR and then the amplicons are analysed by MALDI-TOF MS15.…”

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confidence: 99%

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Identification of different respiratory viruses, after a cell culture step, by matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS)

Calderaro

Arcangeletti

Rodighiero

et al. 2016

Sci Rep

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In this study matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS), a reliable identification method for the diagnosis of bacterial and fungal infections, is presented as an innovative tool to investigate the protein profile of cell cultures infected by the most common viruses causing respiratory tract infections in humans. MALDI-TOF MS was applied to the identification of influenza A and B viruses, adenovirus C species, parainfluenza virus types 1, 2 and 3, respiratory syncytial virus, echovirus, cytomegalovirus and metapneumovirus. In this study MALDI-TOF MS was proposed as a model to be applied to the identification of cultivable respiratory viruses using cell culture as a viral proteins enrichment method to the proteome profiling of virus infected and uninfected cell cultures. The reference virus strains and 58 viruses identified from respiratory samples of subjects with respiratory diseases positive for one of the above mentioned viral agents by cell culture were used for the in vitro infection of suitable cell cultures. The isolated viral particles, concentrated by ultracentrifugation, were used for subsequent protein extraction and their spectra profiles were generated by MALDI-TOF MS analysis. The newly created library allowed us to discriminate between uninfected and respiratory virus infected cell cultures.

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Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 66%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Identification of different respiratory viruses, after a cell culture step, by matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS)

Calderaro

Arcangeletti

Rodighiero

et al. 2016

Sci Rep

Self Cite

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“…Mass spectrometry (MS) and mainly matrixassisted laser desorption ionization-mass spectrometry (MALDI-MS) has grown tremendously as a powerful tool to analyze secondary metabolites of microorganisms 7 and other species such as virus, 8 Imaging mass spectrometry (IMS) that incorporates spatial distribution is an emerging and promising new technology for metabolomics MS analysis. This methodology was introduced in 1997 by Caprioli et al 12 and mostly applied to create 2D images based on the spatial distribution of peptides and protein across the surface of intact biological tissues.…”

Section: Maldi Imaging Mass Spectrometry Of Fresh Water Cyanobacteriamentioning

confidence: 99%

MALDI Imaging Mass Spectrometry of Fresh Water Cyanobacteria: Spatial Distribution of Toxins and Other Metabolites

Sandonato¹,

Santos²,

Luizete³

et al. 2016

Journal of the Brazilian Chemical Society

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Cyanobacteria are among the most ancient forms of life, yet they are known to synthesize highly sophisticated defense molecules, such as the highly hepatotoxic cyclic peptides microcystins and nodularins produced by the genera Microcystis, Anabaena and Nodularia. These metabolites are released by cyanobacteria to water environments causing episodes of fatalities among animals and humans. To better understand the releasing of these metabolites, imaging mass spectrometry (IMS) using matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) was herein applied to determine the spatial distribution of such toxins directly on agar-based cultures. Other key metabolites such as aeruginosin 602 and the siderophore anachelin were also mapped in mixed cyanobacterial cultures, showing the great potential of IMS to spatially monitor the biochemical details of cyanobacterial defense and interactions. Keywords: cyanobacteria, MALDI-TOF, mass spectrometry, imaging IntroductionCyanobacteria are believed to represent one of the most ancient yet highly sophisticated forms of life 1 and these gram-negative oxygenic photosynthetic autotrophic microorganisms are adapted to various ecological habitats such as fresh water, marine, brackish, glaciers, and terrestrial environments.2 Fresh and brackish water cyanobacteria are known to produce a diversity of highly elaborated secondary metabolites, with a truly fascinating variety of structures that exhibit a broad range of applications in the food, nutritional, cosmetic, pharmaceutical and nutraceutical industries. Bloom-forming cyanobacteria (Figure 1), for instance, such as Microcystis aeruginosa, Anabaena cylindrica and Nodularia harveyana, are known to produce a variety of bioactive metabolites.Among these metabolites, cyclic and linear peptides such as microcystin, nodularin, aeruginosin, anabaenopeptin, cyanopeptolin, microginin, cyclamides and microviridin Braz. Chem. Soc. 522 are found, which show activity against various proteases or protein phosphatases. 4 Most of these potent and "dangerous" peptides are released by the cyanobacteria cells into the environment and numerous cases of cyanobacteria poisoning in animals and humans have been reported worldwide.5 Some cyanobacteria also produce iron chelators known as siderophores such as anachelin, schizokinen and synechobactin. 6 There is an interesting behavior of these metabolites, among which the cyclic peptides microcystins and nodularins are such that they are retained inside the cell membrane, whereas anachelin finds its way out in the form of a siderophore, which passes through the membrane into and out of the cell.Mass spectrometry (MS) and mainly matrixassisted laser desorption ionization-mass spectrometry (MALDI-MS) Imaging mass spectrometry (IMS) that incorporates spatial distribution is an emerging and promising new technology for metabolomics MS analysis. This methodology was introduced in 1997 by Caprioli et al. 12 and mostly applied to create 2D images based on the spatial distribution of pe...

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Applications of MALDI‐TOF Mass Spectrometry in Clinical Diagnostic Microbiology

Opota

Prod’hom

Greub

2017

MALDI‐TOF and Tandem MS for Clinical Microbiology

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Until recently, microbial identification in clinical diagnostic laboratories has mainly relied on conventional phenotypic and gene sequencing identification techniques. The development of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) devices has revolutionized the routine identification of microorganisms in clinical microbiology laboratories by introducing an easy, rapid, high throughput, low-cost, and efficient identification technique. This technology has been adapted to the constraint of clinical diagnostic laboratories and has the potential to replace and/or complement conventional identification techniques for both bacterial and fungal strains. Using standardized procedures, the resolution of MALDI-TOF MS allows accurate identification at the species level of most Gram-positive and Gram-negative bacterial strains with the exception of a few difficult strains that require more attention and further development of the method. Similarly, the routine identification by MALDI-TOF MS of yeast isolates is reliable and much quicker than conventional techniques. Recent studies have shown that MALDI-TOF MS has also the potential to accurately identify filamentous fungi and dermatophytes, providing that specific standardized procedures are established for these microorganisms. Moreover, MALDI-TOF MS has been used successfully for microbial typing and identification at the subspecies level, demonstrating that this technology is a potential efficient tool for epidemiological studies and for taxonomical classification.

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Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry applied to virus identification

Cited by 105 publications

References 34 publications

Identification of different respiratory viruses, after a cell culture step, by matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS)

Identification of different respiratory viruses, after a cell culture step, by matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS)

MALDI Imaging Mass Spectrometry of Fresh Water Cyanobacteria: Spatial Distribution of Toxins and Other Metabolites

Applications of MALDI‐TOF Mass Spectrometry in Clinical Diagnostic Microbiology

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