Matrix-assisted laser desorption/ionization time-of-flight analysis ofBacillus spores using a 2.94?�m infrared laser

Ryzhov, Victor; Bundy, Jonathan; Fenselau, Catherine; Taranenko, N. I.; Doroshenko, Vladimir M.; Prasad, Coorg R.

doi:10.1002/1097-0231(20000930)14:18<1701::aid-rcm82>3.0.co;2-i

Cited by 22 publications

(2 citation statements)

References 18 publications

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“…14,17,18 Previously, the identification of Bacillus spp. using MALDI-TOF MS was mainly based on a comparison of mass spectra of proteins, [19][20][21][22][23][24][25][26] lipopeptides, 27-29 and peptides. 30,31 The MALDI-TOF MS method relies on reproducible detection of microbial protein patterns, thus providing complementary information to genotypic or phenotypic test methods.…”

Section: Introductionmentioning

confidence: 79%

Lipid fingerprinting of Bacillus spp. using online MALDI-TOF mass spectrometry

et al. 2012

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Rapid identification of Bacillus spores has attracted great interest in the medical and forensic communities due to the highly pathogenic nature of certain Bacillus species and its potential application in bioweapons. In this study, we evaluated the feasibility of rapid and reliable identification of several Bacillus spp. (B. subtilis, B. cereus, B. megaterium, B. pumilus, and B. sphaericus) using online MALDI-TOF mass spectrometry combined with lipid profiling. The bacterial cultures in liquid medium were extracted with methanol and chloroform, and the extracts were aerosolized into droplets and immediately analyzed with an online MALDI-TOF mass spectrometer. Results showed that phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and diglycosyldiacylglycerol (DGDG) were present in the mass spectra, as alkali metal adducts. The unique lipid profiles obtained from these Bacillus spp. allowed us to differentiate between them. The reliability and reproducibility of the rapid identification method for Bacillus were tested under different experimental conditions. An alternative online chemotaxonomic method for bacterial identification is demonstrated.

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Section: Introductionmentioning

confidence: 79%

Lipid fingerprinting of Bacillus spp. using online MALDI-TOF mass spectrometry

et al. 2012

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“…In contrast, there are many more potential matrices for IR-MALDI because of the strong absorption of molecular compounds in the IR spectral region; the correlation between ion formation and matrix absorption in IR-MALDI is also under discussion [10]. The IR radiation penetrates much deeper into the sample compared to UV, an effect that may be important in some cases like the analysis of Bacillus spores [11].…”

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confidence: 99%

Desorption/ionization of biomolecules from aqueous solutions at atmospheric pressure using an infrared laser at 3 μm

Laiko

Taranenko

Berkout

et al. 2002

J. Am. Soc. Mass Spectrom.

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102

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A new atmospheric pressure (AP) infrared (IR) matrix-assisted laser desorption/ionization (MALDI) ion source was developed and interfaced with a Thermo Finnigan LCQ ion trap mass spectrometer. The source utilized a miniature all-solid-state optical parametric oscillator (OPO)-based IR laser system tunable in the ϭ 1.5-4 m spectral range and a nitrogen ultraviolet (UV) laser ( ϭ 337 nm) for use in comparative studies. The system demonstrated comparable performance at 3 m and 337 nm wavelengths if UV matrices were used. However, AP IR-MALDI using a 3 m wavelength showed good performance with a much broader choice of matrices including glycerol and liquid water. AP IR-MALDI mass spectra of peptides in the mass range up to 2000 Da were obtained directly from aqueous solutions at atmospheric conditions for the first time. A potential use of the new AP IR-MALDI ion source includes direct MS analysis of biological cells and tissues in a normal atmospheric environment as well as on-line coupling of mass spectrometers with liquid separation techniques. (J Am Soc Mass Spectrom 2002, 13, 354 -361)

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2001

J. Mass Spectrom.

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In order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley & Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of mass spectrometry. Each bibliography is divided into 11 sections: 1 Books, Reviews & Symposia; 2 Instrumental Techniques & Methods; 3 Gas Phase Ion Chemistry; 4 Biology/Biochemistry: Amino Acids, Peptides & Proteins; Carbohydrates; Lipids; Nucleic Acids; 5 Pharmacology/Toxicology; 6 Natural Products; 7 Analysis of Organic Compounds; 8 Analysis of Inorganics/Organometallics; 9 Surface Analysis; 10 Environmental Analysis; 11 Elemental Analysis. Within each section, articles are listed in alphabetical order with respect to author (4 Weeks journals ‐ Search completed at 1st. Nov. 2000)

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Matrix-assisted laser desorption/ionization time-of-flight analysis ofBacillus spores using a 2.94?�m infrared laser

Cited by 22 publications

References 18 publications

Lipid fingerprinting of Bacillus spp. using online MALDI-TOF mass spectrometry

Lipid fingerprinting of Bacillus spp. using online MALDI-TOF mass spectrometry

Desorption/ionization of biomolecules from aqueous solutions at atmospheric pressure using an infrared laser at 3 μm

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