Matrix-Assisted Laser Desorption Ionization–Time of Flight (MALDI-TOF) Mass Spectrometry Using the Vitek MS System for Rapid and Accurate Identification of Dermatophytes on Solid Cultures

Respinis, Sophie De; Monnin, Valérie; Girard, Victoria; Welker, Martin; Arsac, Maud; Cellière, Béatrice; Durand, G.; Bosshard, Philipp P.; Farina, Claudio; Passera, Marco; Belkum, Alex van; Petrini, Orlando; Tonolla, Mauro

doi:10.1128/jcm.02199-14

Cited by 57 publications

(58 citation statements)

References 35 publications

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“…Previous studies have shown that MALDI-TOF MS is a rapid and accurate method for the identification of dermatophytes. There have been many studies examining this group of molds, with identification rates of Ͼ90% for these studies (12,13,(20)(21)(22). Our study correctly identified 100% (14/14) of the dermatophytes tested.…”

Section: Discussionmentioning

confidence: 56%

“…Prior studies have evaluated the accuracy of MALDI-TOF MS for the identification of only a few genera or groups of organisms, such as Aspergillus spp. or the dermatophytes (12,13,(19)(20)(21)(22)(23)(24)(25)(26). In our study, which examined 319 isolates from 43 different genera, the Vitek MS version 3.0 library was able to correctly identify 66.8% of isolates and 76.8% of the isolates after additional SARAMIS analysis.…”

Section: Discussionmentioning

confidence: 76%

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Evaluation of the Vitek MS Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Clinically Relevant Filamentous Fungi

et al. 2016

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bInvasive fungal infections have a high rate of morbidity and mortality, and accurate identification is necessary to guide appropriate antifungal therapy. With the increasing incidence of invasive disease attributed to filamentous fungi, rapid and accurate species-level identification of these pathogens is necessary. Traditional methods for identification of filamentous fungi can be slow and may lack resolution. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a rapid and accurate method for identification of bacteria and yeasts, but a paucity of data exists on the performance characteristics of this method for identification of filamentous fungi. The objective of our study was to evaluate the accuracy of the Vitek MS for mold identification. A total of 319 mold isolates representing 43 genera recovered from clinical specimens were evaluated. Of these isolates, 213 (66.8%) were correctly identified using the Vitek MS Knowledge Base, version 3.0 database. When a modified SARAMIS (Spectral Archive and Microbial Identification System) database was used to augment the version 3.0 Knowledge Base, 245 (76.8%) isolates were correctly identified. Unidentified isolates were subcultured for repeat testing; 71/ 319 (22.3%) remained unidentified. Of the unidentified isolates, 69 were not in the database. Only 3 (0.9%) isolates were misidentified by MALDI-TOF MS (including Aspergillus amoenus [n ‫؍‬ 2] and Aspergillus calidoustus [n ‫؍‬ 1]) although 10 (3.1%) of the original phenotypic identifications were not correct. In addition, this methodology was able to accurately identify 133/144 (93.6%) Aspergillus sp. isolates to the species level. MALDI-TOF MS has the potential to expedite mold identification, and misidentifications are rare. Filamentous fungi are ubiquitous environmental microorganisms that have become increasingly important pathogens, especially in immunocompromised patients. Invasive fungal diseases have a high rate of morbidity and mortality, and accurate identification is necessary to guide appropriate antifungal therapy. Traditionally, the identification of filamentous fungi has required the use of different phenotypic methods in conjunction with macro-and microscopic assessment of the organism (1). This process requires highly trained mycologists, and, in some cases, turnaround time may be prolonged due to a requirement for extended incubation periods, potentially delaying appropriate therapy. Molecular methods, which can provide accurate identification to the species level, can be expensive, require specialized equipment or expertise, and are not commonly available in clinical laboratories.Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been utilized in the clinical microbiology laboratory for rapid and accurate identification of bacteria, mycobacteria, and yeasts (2-9). For filamentous fungi, databases have been limited, and unlike bacteria, filamentous fungi require additional processing steps to dis...

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Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 76%

Evaluation of the Vitek MS Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Clinically Relevant Filamentous Fungi

et al. 2016

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“…Finally, the expanded database was challenged with 131 clinical isolates of dermatophytes belonging to 13 taxa. For 10 taxa, the rate of correct identifications was 100%, including Trichophyton erinacei (1 isolate) and Trichophyton terrestre (1 isolate); in contrast, only 15 of 19 (78.9%) African Trichophyton rubrum (also called Trichophyton soudanense) isolates were correctly identified (this rate was clearly lower than that for T. rubrum sensu stricto isolates [100%]), with several isolates being misidentified as Trichophyton violaceum, demonstrating the close relationship of these two taxa (31). Later, in another study, the Bruker Biotyper database was supplemented with additional mass spectra from 24 (13 reference [United Kingdom National External Quality Assessment Service {UK NEQAS}] and 11 clinical) morphologically and/or molecularly (i.e., by ITS DNA sequencing) characterized strains of 13 different species (32).…”

Section: Rhizopus-lichtheimia Species Previous Reports On the Maldi-mentioning

confidence: 92%

“…Although these methods can provide accurate results, they are expensive, require specialized equipment or expertise, and are not commonly available in clinical laboratories. In the present review, findings from the 10 studies (23)(24)(25)(27)(28)(29)(30)(31)(32)(33) shown in Table 1 indicate that before MALDI-TOF MS testing, only one study identified isolates via conventional phenotypical analyses (33), whereas almost all the remaining studies also sequenced all their isolates, and in two studies, only molecular biology analyses of the isolates were undertaken (27,28). This supports the idea that the overall high level of accuracy of MALDI-TOF MS-based mold identification shown in these studies might not be overestimated.…”

Section: Mold Identification By Maldi-tof Ms: Is It Really a History mentioning

confidence: 99%

“…As expected, all these studies established their own reference dermatophyte database. In one study, using a combination of organism inactivation and protein extraction, De Respinis et al developed a sample preparation protocol that allowed users to obtain rapid, reliable, and reproducible mass spectra from fungal material grown on solid media (i.e., material derived from primary cultures of skin, nails, or hairs) (31). An expansion of the Vitek MS V2.0.0 fungal knowledge base was then made by using 134 well-characterized strains from 17 dermatophyte species, for 1,130 dermatophyte mass spectra overall, that gave an estimated identification performance of 98.4%.…”

Section: Rhizopus-lichtheimia Species Previous Reports On the Maldi-mentioning

confidence: 99%

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Identification of Molds by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Sanguinetti

2017

J Clin Microbiol

121

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Although to a lesser extent than diagnostic bacteriology, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has recently revolutionized the diagnostic mycology workflow. With regard to filamentous fungi (or molds), the precise recognition of pathogenic species is important for rapid diagnosis and appropriate treatment, especially for invasive diseases. This review summarizes the current experience with MALDI-TOF MS-based identification of common and uncommon mold species of Aspergillus, Fusarium, Mucorales, dimorphic fungi, and dermatophytes. This experience clearly shows that MALDI-TOF MS holds promise as a fast and accurate identification tool, particularly with common species or typical strains of filamentous fungi.

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Applications of MALDI‐TOF Mass Spectrometry in Clinical Diagnostic Microbiology

Opota

Prod’hom

Greub

2017

MALDI‐TOF and Tandem MS for Clinical Microbiology

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Until recently, microbial identification in clinical diagnostic laboratories has mainly relied on conventional phenotypic and gene sequencing identification techniques. The development of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) devices has revolutionized the routine identification of microorganisms in clinical microbiology laboratories by introducing an easy, rapid, high throughput, low-cost, and efficient identification technique. This technology has been adapted to the constraint of clinical diagnostic laboratories and has the potential to replace and/or complement conventional identification techniques for both bacterial and fungal strains. Using standardized procedures, the resolution of MALDI-TOF MS allows accurate identification at the species level of most Gram-positive and Gram-negative bacterial strains with the exception of a few difficult strains that require more attention and further development of the method. Similarly, the routine identification by MALDI-TOF MS of yeast isolates is reliable and much quicker than conventional techniques. Recent studies have shown that MALDI-TOF MS has also the potential to accurately identify filamentous fungi and dermatophytes, providing that specific standardized procedures are established for these microorganisms. Moreover, MALDI-TOF MS has been used successfully for microbial typing and identification at the subspecies level, demonstrating that this technology is a potential efficient tool for epidemiological studies and for taxonomical classification.

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Matrix-Assisted Laser Desorption Ionization–Time of Flight (MALDI-TOF) Mass Spectrometry Using the Vitek MS System for Rapid and Accurate Identification of Dermatophytes on Solid Cultures

Cited by 57 publications

References 35 publications

Evaluation of the Vitek MS Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Clinically Relevant Filamentous Fungi

Evaluation of the Vitek MS Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Clinically Relevant Filamentous Fungi

Identification of Molds by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Applications of MALDI‐TOF Mass Spectrometry in Clinical Diagnostic Microbiology

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