Matrix-assisted laser desorption/ionisation aerosol time-of-flight mass spectrometry for the analysis of bioaerosols: development of a fast detector for airborne biological pathogens

Wuijckhuijse, A.L. van; Stowers, M.A.; Kleefsman, W.A.; Baar, Ben L. M. van; Kientz, Ch.E.; Marijnissen, J.C.M.

doi:10.1016/j.jaerosci.2004.11.003

Cited by 74 publications

(66 citation statements)

References 27 publications

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“…A wave pass filter allowing light between 290 and 500 nm is placed in front of the photomultiplier tube. The collection time of the fluorescence by the PMT equals the residence time of the particles in the UV laser beam, which is approximately 1 µs 11,16,21) . The time between the PMT output pulses is also used to trigger the Excimer ionization laser (ATL Lasertechnics, Friedrichstal, Germany; Model EC50), which is focused at 0.5 mm below the second detection beam.…”

Section: Methodsmentioning

confidence: 99%

“…10,11,21) Stowers et al 10) reported the on-line aerosol MALDI MS analysis of Bacillus atrophaeus spores and found a reproducible peak at a mass of 1224 Dalton, which was attributed to a part of peptidoglycan. The work done by Van Wuijckhuijse 11,21) included the on-line aerosol MALDI analysis of spores of Bacillus atrophaeus and of vegetative cells of Escherichia coli. The obtained mass spectra covered a mass range up to 10 kDa.…”

Section: Single Particle Mass Spectrometry Bioaerosol Analysis By Malmentioning

confidence: 99%

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Single Particle Mass Spectrometry–Bioaerosol Analysis by MALDI MS

Kleefsman

Stowers

Verheijen

et al. 2008

KONA

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Section: Methodsmentioning

confidence: 99%

Section: Single Particle Mass Spectrometry Bioaerosol Analysis By Malmentioning

confidence: 99%

Single Particle Mass Spectrometry–Bioaerosol Analysis by MALDI MS

Kleefsman

Stowers

Verheijen

et al. 2008

KONA

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“…In 2005, Harris et al described aerosol MALDI in an ion trap mass spectrometer [36]. A number of groups have analyzed proteins, but in 2000, Stowers et al were the first to analyze Bacillus spores [37,38]. Regardless of the analyte, aerosol MALDI differs from classical bulk MALDI in a number of important ways.…”

mentioning

confidence: 99%

Parameters contributing to efficient ion generation in aerosol MALDI mass spectrometry

McJimpsey

Jackson

Lebrilla

et al. 2008

J. Am. Soc. Mass Spectrom.

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The Bioaerosol Mass Spectrometry (BAMS) system was developed for the real-time detection and identification of biological aerosols using laser desorption ionization. Greater differentiation of particle types is desired; consequently MALDI techniques are being investigated. The small sample size (ϳ1 m 3 ), lack of substrate, and ability to simultaneously monitor both positive and negative ions provide a unique opportunity to gain new insight into the MALDI process. Several parameters known to influence MALDI molecular ion yield and formation are investigated here in the single particle phase. A comparative study of five matrices (2,6-dihydroxyacetophenone, 2,5-dihydroxybenzoic acid, ␣-cyano-4-hydroxycinnamic acid, ferulic ␣ acid, and sinapinic acid) with a single analyte (angiotensin I) is presented and reveals effects of matrix selection, matrix-to-analyte molar ratio, and aerosol particle diameter. The strongest analyte ion signal is found at a matrix-to-analyte molar ratio of 100:1. At this ratio, the matrices yielding the least and greatest analyte molecular ion formation are ferulic acid and ␣-cyano-␣ 4-hydroxycinnamic acid, respectively. Additionally, a significant positive correlation is found between aerodynamic particle diameter and analyte molecular ion yield for all matrices. SEM imaging of select aerosol particle types reveals interesting surface morphology and structure.

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“…Over the past decade, MALDI has developed into a powerful tool for mass spectrometric analysis of biomolecules. It is particularly well suited for mass analysis of peptides and proteins since it allows the generation of molecular ions from large mass compounds that would otherwise fragment under normal laser irradiation [15][16][17][18][19] . In MALDI, the analyte of interest is mixed with a large mole excess of a matrix that absorbs efficiently at the laser wavelength and allows the energy from the laser to be dissipated 20 .…”

Section: Results From the Linear Mass Spectrometer And Maldimentioning

confidence: 99%

“…The optimal experimental parameters realized from these studies will ultimately be used to develop dual polarity, high mass signatures of Bacillus spores and vegetative cells, which may be coated with a matrix on-the-fly 18,22 . It is hoped that these modifications and experiments will ultimately make BAMS an even more powerful tool for bioaerosol detection as well as a more useful tool for fundamental research.…”

Section: Results From the Linear Mass Spectrometer And Maldimentioning

confidence: 99%

Detection of biological particles in ambient air using bioaerosol mass spectrometry

et al. 2006

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The Bio-Aerosol Mass Spectrometry (BAMS) system is an instrument used for the real time detection and identification of biological aerosols. Particles are drawn from the atmosphere directly into vacuum and tracked as they scatter light from several continuous wave lasers. After tracking, the fluorescence of individual particles is excited by a pulsed 266nm or 355nm laser. Molecules from those particles with appropriate fluorescence properties are subsequently desorbed and ionized using a pulsed 266nm laser. Resulting ions are analyzed in a dual polarity mass spectrometer. During two field deployments at the San Francisco International Airport, millions of ambient particles were analyzed and a small but significant fraction were found to have fluorescent properties similar to Bacillus spores and vegetative cells. Further separation of non-biological background particles from potential biological particles was accomplished using laser desorption/ionization mass spectrometry. This has been shown to enable some level of species differentiation in specific cases, but the creation and observation of higher mass ions is needed to enable a higher level of specificity across more species. A soft ionization technique, matrix-assisted laser desorption/ionization (MALDI) is being investigated for this purpose. MALDI is particularly well suited for mass analysis of biomolecules since it allows for the generation of molecular ions from large mass compounds that would fragment under normal irradiation. Some of the initial results from a modified BAMS system utilizing this technique are described.

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Matrix-assisted laser desorption/ionisation aerosol time-of-flight mass spectrometry for the analysis of bioaerosols: development of a fast detector for airborne biological pathogens

Cited by 74 publications

References 27 publications

Single Particle Mass Spectrometry–Bioaerosol Analysis by MALDI MS

Single Particle Mass Spectrometry–Bioaerosol Analysis by MALDI MS

Parameters contributing to efficient ion generation in aerosol MALDI mass spectrometry

Detection of biological particles in ambient air using bioaerosol mass spectrometry

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