Matrisomal components involved in regenerative wound healing in axolotl and <i>Acomys</i>: implications for biomaterial development

Avila-Martinez, Nancy; Gansevoort, Merel; Verbakel, Juul; Jayaprakash, Haarshaadri; Araujo, Ines Maria; Vitorino, Marta; Tiscornia, Gustavo; van Kuppevelt, Toin H.; Daamen, Willeke F.

doi:10.1039/d3bm00835e

Cited by 1 publication

(2 citation statements)

References 186 publications

(309 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The incredible regenerative ability of both Acomys and axolotls provides a unique opportunity for identification of ECM components which contribute to their shared regenerative capacity. A recent review compared expression of ECM-related genes between Acomys and axolotl and identified a number of shared expression patterns that may contribute to regeneration in these models, including shared upregulation of COL12A1, TNC, FN1, THBS1, THBS2, and C1QC between the two species 124 . All proteins reported as upregulated in both Acomys and axolotl display concurrent upregulation in our data, demonstrating strong agreement between our study and previous analyses.…”

Section: While Cell Markers Indicate Greater Macrophage Contribution ...mentioning

confidence: 99%

“…All proteins reported as upregulated in both Acomys and axolotl display concurrent upregulation in our data, demonstrating strong agreement between our study and previous analyses. However, analyses incorporated into this review were limited to transcriptomics and analysis of the soluble ECM fraction 124 , limiting the conclusions that can be drawn regarding additional alterations to the insoluble, fibrillar ECM that we are able to capture in this study.…”

Section: While Cell Markers Indicate Greater Macrophage Contribution ...mentioning

confidence: 99%

See 1 more Smart Citation

ECM-Focused Proteomic Analysis of Ear Punch Regeneration inAcomys Cahirinus

McCabe,

Okamura,

Erickson

et al. 2023

Preprint

View full text Add to dashboard Cite

In mammals, significant injury is generally followed by the formation of a fibrotic scar which provides structural integrity but fails to functionally restore damaged tissue. Spiny mice of the genus Acomys represent the first example of full skin autotomy in mammals. Acomys cahirinus has evolved extremely weak skin as a strategy to avoid predation and is able to repeatedly regenerate healthy tissue without scar after severe skin injury or full-thickness ear punches. Extracellular matrix (ECM) composition is a critical regulator of wound repair and scar formation and previous studies have suggested that alterations in its expression may be responsible for the differences in regenerative capacity observed between Mus musculus and A. cahirinus, yet analysis of this critical tissue component has been limited in previous studies by its insolubility and resistance to extraction. Here, we utilize a 3-step ECM-optimized extraction to perform proteomic analysis of tissue composition during wound repair after full-thickness ear punches in A. cahirinus and M. musculus from weeks 1 to 4 post-injury. We observe changes in a wide range of ECM proteins which have been previously implicated in wound regeneration and scar formation, including collagens, coagulation and provisional matrix proteins, and matricryptic signaling peptides. We additionally report differences in crosslinking enzyme activity and ECM protein solubility between Mus and Acomys. Furthermore, we observed rapid and sustained increases in CD206, a marker of pro-regenerative M2 macrophages, in Acomys, whereas little or no increase in CD206 was detected in Mus. Together, these findings contribute to a comprehensive understanding of tissue cues which drive the regenerative capacity of Acomys and identify a number of potential targets for future pro-regenerative therapies.

show abstract

Section: While Cell Markers Indicate Greater Macrophage Contribution ...mentioning

confidence: 99%

Section: While Cell Markers Indicate Greater Macrophage Contribution ...mentioning

confidence: 99%

ECM-Focused Proteomic Analysis of Ear Punch Regeneration inAcomys Cahirinus

McCabe,

Okamura,

Erickson

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Matrisomal components involved in regenerative wound healing in axolotl and Acomys: implications for biomaterial development

Abstract: Acomys and axolotl share key regenerative components of the matrisome and glycosaminoglycan biosynthesis during scarless wound healing. Addition of key matrisomal components to biomaterials may improve skin wound healing in non-regenerating mammals.

Cited by 1 publication

References 186 publications

ECM-Focused Proteomic Analysis of Ear Punch Regeneration inAcomys Cahirinus

ECM-Focused Proteomic Analysis of Ear Punch Regeneration inAcomys Cahirinus

Contact Info

Product

Resources

About