Matrine reduces yeast-to-hypha transition and resistance of a fluconazole-resistant strain of <i>Candida albicans</i>

Shao, Jing; Wang, T.; Yan, Yuanyuan; Shi, Guoping; Cheng, Hongwei; Wu, Donghui; Wang, C.

doi:10.1111/jam.12555

Cited by 31 publications

(19 citation statements)

References 34 publications

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“…The procedures of qRT-PCR analysis were described in a previous study of our group (Shao et al. 2014 ). Briefly, 1 mL C. albicans 1601 strain broth (= 1 × 10 6 CFU/mL) was mixed with 8 μg/mL fluconazole and/or 16 μg/mL SH at 37 °C for 24 h into a sterilized, flat-bottomed 24-well polystyrene microtiter plate (Corning, NY).…”

Section: Methodsmentioning

confidence: 99%

Synergistic in vitro activity of sodium houttuyfonate with fluconazole against clinical Candida albicans strains under planktonic growing conditions

Shao

Cui

Zhang

et al. 2016

Pharmaceutical Biology

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Context: Fluconazole resistance is an intractable problem of treating Candida albicans, calling for more antifungal agents to enhance the activity of fluconazole.Objective: This work investigates the anti-C. albicans activities of sodium houttuyfonate (SH) and/or fluconazole and the associated mechanism.Materials and methods: The minimum inhibitory concentrations (MICs) of SH and fluconazole both ranging from 0.5 to 1024 μg/mL were determined by broth microdilution method in 19 C. albicans isolates, and their fractional inhibitory concentration index (FICI) was evaluated by checkerboard assay. After MICSH and/or MICfluconazole treatments, the expressions of IFD6, PHR1, ZAP1, ADH5, BGL2, XOG1 and FKS1 were analyzed by quantitative reverse transcription polymerase chain reaction (qRT-PCR) in C. albicans 1601.Results and conclusion: The MICs of SH alone ranged from 32 to 256 μg/mL and decreased 2–16-fold in combination. SH showed strong synergism with fluconazole with FICI <0.13–0.5. In C. albicans 1601, we observed that (i) the expression of the seven genes increased notably in a range between 3.71- and 12.63-fold (p < 0.05) when SH was used alone, (ii) the combined use of SH and fluconazole slightly inhibited the expression of IFD6 and PHR1 by 1.23- and 1.35-fold (p > 0.05), but promoted evidently the expression of ZAP1, ADH5, XOG1 and FKS1 by 1.98-, 3.56-, 4.10- and 2.86-fold (p < 0.05). The results suggested SH to be a potential synergist to enhance the antifungal activity of fluconazole in C. albicans resistant isolates, and the underlying mechanism may be associated with β-1,3-glucan synthesis and transportation.

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Section: Methodsmentioning

confidence: 99%

Synergistic in vitro activity of sodium houttuyfonate with fluconazole against clinical Candida albicans strains under planktonic growing conditions

Shao

Cui

Zhang

et al. 2016

Pharmaceutical Biology

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“…The procedures of qRT-PCR analysis were described in a previous study of our group (Shao et al 2014). Briefly, total RNA samples from Ca z2003 were extracted according to the instructions of MagExtractor-RNA kit (ToyoBo, Tokyo, Japan).…”

Section: Cdr1 Cdr2 and Mdr1 Expression Analysed By Qrt-pcrmentioning

confidence: 99%

The roles of CDR1, CDR2, and MDR1 in kaempferol-induced suppression with fluconazole-resistant Candida albicans

Shao

Zhang

Wang

et al. 2015

Pharmaceutical Biology

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Context: Fungal infections caused by fluconazole-resistant Candida albicans are an intractable clinical problem, calling for new efficient antifungal drugs. Kaempferol, an active flavonoid, has been considered a potential candidate against Candida species. Objective: This work investigates the resistance reversion of kaempferol in fluconazole-resistant C. albicans and the underlying mechanism. Materials and methods: The antifungal activities of fluconazole and/or kaempferol were assessed by a series of standard procedures including broth microdilution method, checkerboard assay and time-kill (T-K) test in nine clinical strains as well as a standard reference isolate of C. albicans. Subsequently, the morphological changes, the efflux of rhodamine 6G, and the expressions of CDR 1, CDR 2, and MDR 1 were analysed by scanning electron microscope (SEM), inverted fluorescence microscope and quantitative reverse transcription polymerase chain reaction (qRT-PCR) in C. albicans z2003.Results: For all the tested C. albicans strains, the minimum inhibitory concentrations (MICs) of fluconazole and kaempferol ranged 0.25-32 and 128-256 mg/mL with a range of fractional inhibitory concentration index of 0.257-0.531. In C. albicans z2003, the expression of both CDR 1 and CDR 2 were decreased after exposure to kaempferol alone with negligible rhodamine 6G accumulation, while the expression of CDR 1, CDR 2 and MDR 1 were all decreased when fluconazole and kaempferol were used concomitantly with notable fluorescence of rhodamine 6G observed. Discussion and conclusion: Kaempferol-induced reversion in fluconazole-resistant C. albicans might be likely due to the suppression of the expression of CDR1, CDR2 and MDR1.ARTICLE HISTORY

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“…matrine [39], oxymatrine [40], gallic acid [41]), but usually owned nice in vivo antifungal functions (e.g. sodium houttuyfonate [42], baicalein [43]).…”

Section: Discussionmentioning

confidence: 99%

In vitro antifungal activity of baicalin against Candida albicans biofilms via apoptotic induction

Wang

Shi

Shao

et al. 2015

Microbial Pathogenesis

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Matrine reduces yeast-to-hypha transition and resistance of a fluconazole-resistant strain of Candida albicans

Abstract: More efforts are needed to find new antifungal agents. Matrine could be a potential candidate to fight against Candida-related infections by regulating yeast-to-hypha transition.

Cited by 31 publications

References 34 publications

Synergistic in vitro activity of sodium houttuyfonate with fluconazole against clinical Candida albicans strains under planktonic growing conditions

Synergistic in vitro activity of sodium houttuyfonate with fluconazole against clinical Candida albicans strains under planktonic growing conditions

The roles of CDR1, CDR2, and MDR1 in kaempferol-induced suppression with fluconazole-resistant Candida albicans

In vitro antifungal activity of baicalin against Candida albicans biofilms via apoptotic induction

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