Matrine inhibits the proliferation, invasion and migration of castration-resistant prostate cancer cells through regulation of the NF-κB signaling pathway

Li, Qi; Lai, Yue‐Yun; Wang, Chengbin; Xu, Guibin; He, Zheng; Shang, Xianwen; Sun, Yi; Zhang, Fan; Liu, Leyuan; Huang, Hai

doi:10.3892/or.2015.4341

Cited by 32 publications

(28 citation statements)

References 35 publications

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“…In our previous study, it has been proved that matrine could inhibit the proliferation of PC-3 and DU145 cells dose-dependently and time-dependently. 8,10 In the present study, we evaluated the antitumor effect of matrine in combination with docetaxel on androgen-independent prostate cancer cells DU145 and PC-3. We showed that matrine combined with docetaxel is effective to inhibit the proliferation, migration, and invasion of both DU145 and PC3 cells to some extent.…”

Section: Discussionmentioning

confidence: 99%

“…The invasion assay were performed as previously described in a published study. 10 We seeded cells 24 hrs after treatment with matrine and docetaxel for 48 hrs. After seeding, the MEMF12 medium (c11330500bt; Invitrogen, Life Technologies) in the upper chambers was aspirated and replaced with serum-free medium and added 10% fetal bovine serum to the bottom chamber.…”

Section: In Vitro Invasion Assaymentioning

confidence: 99%

“…Western blot analysis was performed as previously described. 10 The protein was boiled and subjected to Western blot with antibodies against caspase-1 48 kDa, caspase-1 20 kDa and 1:1000 GAPDH (Kangcheng Biology, Shanghai, China). The blots were exposed, stained with ECL Plus (Millipore) and visualized on X-ray film.…”

Section: Western Blot Analysismentioning

confidence: 99%

“…8 Our previous studies had shown that matrine can inhibit cell proliferation, migration, and invasion, and induce apoptosis in both DU145 and PC-3 cells. 9,10 Although matrine combined with docetaxel has been used clinically to treat castration-resistant prostate cancer in recent years in China, however, the mechanismic research of matrine combined with docetaxel for the treatment of castration-resistant prostate cancer is less clear. In the present study, our aim was to explore the effects of matrine in combination with docetaxel on castration-resistant PC.…”

Section: Introductionmentioning

confidence: 99%

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<p>The Effects of Matrine in Combination with Docetaxel on Castration-Resistant (Androgen-Independent) Prostate Cancer</p>

Li¹,

Xu²,

He³

et al. 2019

CMAR

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Background: Matrine (MAT) exhibits higher efficacy of chemotherapy when it is combined with other chemotherapeutic drugs; however, the therapeutic efficacy of matrine in combination with docetaxel (DOC) for prostate cancer, or even androgen-independent prostate cancer, remains poorly understood and the underlying molecular mechanisms have not yet been clearly defined. In the present study, we investigated whether matrine combined with docetaxel can strengthen anti-cancer effect. Methods: In this study, 7 groups were established, including (1) blank control group (cells).(2) 0.1 g/L MAT group, (3) 0.5 g/L MAT group, (4) 0.1 g/L MAT+ 50 μg/L DOC group, (5) 0.5 g/L MAT+ 50 μg/L DOC group, (6) 0.1 g/L MAT+ 100 μg/L DOC group, and (7) 0.5 g/L MAT+ 100 μg/L DOC group. MTS assay was performed to detect the anti-proliferative effects of each group on DU145 and PC-3 cells. At the same time, Transwell assay was performed to detect anti-migrative and anti-invasive effects of each group on DU145 and PC-3 cells. Biochemical colorimetric method and enzyme-linked immunosorbent assay were performed to detect the levels of LDH, IL-1β and IL-18 of each group on DU145 and PC-3 cells. Flow cytometry (FCM) assay was used to do the apoptosis analysis on DU145 and PC-3 cells of each group. At last, Western blot analysis was performed to investigate the expression levels of caspase1 in cells of each group. Statistical analyses were performed with SPSS 17.0 (SPSS Inc, USA) software, and one-way ANOVA and Fisher's exact test was taken. Results: MTS assay showed that matrine combined with docetaxel could inhibit both DU145 and PC-3 cells' proliferation in a dose-and time-dependent manner. Transwell assay showed that matrine combined with docetaxel could inhibit both DU145 and PC-3 cells' migration and invasion in a dose-and time-dependent manner. The levels of LDH, IL-1β and IL-18 of matrine combined with docetaxel-treated DU145 and PC-3 cells were significantly increased, compared with the untreated control cells. Flow cytometry, as well as Annexin-V/PI staining, showed a significant and dose-dependent increase in the number of early, as well as late-stage apoptotic cells in both DU145 and PC-3 cells compared with the untreated control cells. Western blot analysis showed that matrine combined with docetaxel treatment led to the expression of caspase1 in both DU145 and PC-3 cells. Conclusion: It may be more effective to use matrine in combination with docetaxel to treat androgen-resistant prostate cancer because matrine can help to affect proliferation, migration, invasion, apoptosis, metabolism, and have anti-inflammation effect on the tumor cells.

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Section: Discussionmentioning

confidence: 99%

Section: In Vitro Invasion Assaymentioning

confidence: 99%

Section: Western Blot Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

<p>The Effects of Matrine in Combination with Docetaxel on Castration-Resistant (Androgen-Independent) Prostate Cancer</p>

Li¹,

Xu²,

He³

et al. 2019

CMAR

Self Cite

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“…In the present study, it was also found that sophoridine significantly decreases the protein expression of NF-κB in D283-Med cells. Li et al (23) reported that sophoridine inhibits cell proliferation through regulation of the NF-κB signaling pathway in castration-resistant prostate cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Sophoridine suppresses cell growth in human medulloblastoma through FoxM1, NF‑κB and AP‑1

Yue

Pan

et al. 2017

Oncol Lett

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Abstract. Sophoridine is an alkaloid extracted from Sophora alopecuroides that has extensive pharmacological actions. In the present study, the effect of sophoridine on cell growth of human medulloblastoma and its mechanism were investigated. Human medulloblastoma D283-Med cells were incubated with 0, 0.5, 1 or 2 mg/ml sophoridine for 24, 48 or 72 h. Cell proliferation and cytotoxicity were analyzed using MTT and lactate dehydrogenase assays, respectively. Next, analyses of cell apoptosis and caspase-3/8 activity were performed using flow cytometry or spectrophotometry, respectively. Lastly, the change in FoxM1, TrkB, BDNF, NF-κB and AP-1 expression was investigated using western blot analysis. In the present study, treatment with sophoridine significantly suppressed cell growth and induced apoptosis in human medulloblastoma cells. In addition, sophoridine significantly increased cytotoxicity and caspase-3/8 activity in human medulloblastoma. Finally, it was found that sophoridine suppresses the protein expression of FoxM1, TrkB, BDNF NF-κB and AP-1 in human medulloblastoma cells. The present study suggests that sophoridine suppresses cell growth of human medulloblastoma through the inhibition of the FoxM1, NF-κB and AP-1 signaling pathway.

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UHRF1 is regulated by miR‐124‐3p and promotes cell proliferation in intrahepatic cholangiocarcinoma

Zhu

Wei

Lin

et al. 2019

Journal Cellular Physiology

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Ubiquitin-like with PHD and ring finger domains 1 (UHRF1) is abnormally overexpressed in multiple cancers and closely correlated with tumor-promoting effects, such as high proliferation. However, how UHRF1 functions in intrahepatic cholangiocarcinoma (ICC) has not yet been determined. Herein, we found that UHRF1 is overexpressed in ICC tissues. Downregulated UHRF1 attenuated the transition of the G1/S cell cycle and then suppressed cell proliferation in vitro and tumor growth in vivo. Moreover, upstream regulators of the UHRF1 expression were predicted, and we found that direct binding of miR-124-3p inhibited the UHRF1 expression. Elevated miR-124-3p suppressed proliferation and led to the arrest of the cell cycle. Furthermore, the expression of UHRF1 was positively correlated with PCNA. Clinically, we showed that elevated UHRF1 was associated with poor prognosis, and served as an independent prognostic factor in ICC patients. Together, these findings demonstrate that UHRF1, regulated by miR-124-3p, acts as a tumor promoter by promoting cell proliferation in ICC. K E Y W O R D Scell cycle, ICC, miR-124-3p, prognosis, proliferation, UHRF1

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Matrine inhibits the proliferation, invasion and migration of castration-resistant prostate cancer cells through regulation of the NF-κB signaling pathway

Cited by 32 publications

References 35 publications

<p>The Effects of Matrine in Combination with Docetaxel on Castration-Resistant (Androgen-Independent) Prostate Cancer</p>

<p>The Effects of Matrine in Combination with Docetaxel on Castration-Resistant (Androgen-Independent) Prostate Cancer</p>

Sophoridine suppresses cell growth in human medulloblastoma through FoxM1, NF‑κB and AP‑1

UHRF1 is regulated by miR‐124‐3p and promotes cell proliferation in intrahepatic cholangiocarcinoma

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