Matrine inhibits the proliferation and migration of lung cancer cells through regulation of the protein kinase�B/glycogen synthase kinase‑3β signaling pathways

Abstract

Lung cancer is the leading cause of cancer-related mortality worldwide. Despite recent advances in treatment, lung cancer remains an incurable disease. Matrine, an active compound isolated from Sophora flavescens, has been demonstrated to inhibit proliferation and induce apoptosis of tumor cells. However, the protective effects and molecular mechanisms of matrine in lung cancer remain elusive. In the present study, the lung cancer cells H1299 and A549 were used to investigate how matrine affects the proliferation, migration and apoptosis of lung cancer cells in vitro. It was demonstrated that matrine is able to significantly suppress the proliferation and colony formation of lung cancer cells in vitro. Using cell apoptosis analysis, wound-healing and Transwell assays, it was demonstrated that matrine induced cellular apoptosis and inhibited the migration of lung cancer cells. Further experiments revealed that matrine significantly suppressed the phosphorylation of protein kinase B (Akt) and glycogen synthase kinase-3β (GSK-3β). The present results suggested that matrine inhibits lung cancer cell proliferation, and induces cell apoptosis by suppressing the Akt/GSK-3β signaling pathway, which demonstrated that matrine may have therapeutic potential for lung cancer.

Paeoniflorin Inhibits Mesangial Cell Proliferation and Inflammatory Response in Rats With Mesangial Proliferative Glomerulonephritis Through PI3K/AKT/GSK-3β Pathway

“…Additionally, one study reported that phosphorylation of AKT is involved in the initiation and development of mesangial proliferation in anti-Thy1 MPGN model rats (Qiu et al, 2012). GSK-3β, a major downstream signaling molecule of AKT participating in various cellular processes, plays a crucial role in inflammation and proliferation (Hong et al, 2017; Xie et al, 2018). More importantly, GSK-3 inhibitors may emerge as effective drugs for proliferative kidney diseases (Soos et al, 2006).…”

<p>Matrine Restrains Cell Growth and Metastasis by Up-Regulating LINC00472 in Bladder Carcinoma</p>

“…For instance, a research pointed out that MAT restrained cell proliferation in pancreatic carcinoma 27 while the other research pointed out that MAT restrained cell migration in lung carcinoma. 28 More interesting, in an animal model of bladder tumor, Gao et al pointed out that MAT could suppress bladder tumor invasion via regulation of the cyclooxygenase-2 (COX-2), and cytosolic recombinant phospholipase A2 (cPLA2). 29 MAT treatment induced the inhibition of cell proliferation and invasion of bladder cancer, which was mediated by the PI3K/AKT signaling pathway, in vitro.…”

Matrine Exerts Pharmacological Effects Through Multiple Signaling Pathways: A Comprehensive Review

“…This study demonstrated that mechanistically, the growth inhibitory effects of matrine were dependent on the suppression of PI3K/AKT and the upregulation of caveolae associated protein 3 (Cavin3). Xie et al 15 showed that matrine significantly suppressed proliferation and colony formation, induced cellular apoptosis, and inhibited the migration of lung cancer cells, and further found that matrine markedly inhibited the phosphorylation of AKT and glycogen synthase kinase-3β (GSK-3β), indicating that the inhibitory effects of matrine in lung cancer are possibly through suppression of the AKT/GSK-3β signaling pathway. Liao et al 16 found that matrine treatment significantly inhibited the proliferation, migration, and invasion of human NCI-H1299 lung cancer cells in a concentration-dependent manner, and the mechanism may be related to the upregulation of microRNA (miR)-133a expression and inhibition of the epidermal growth factor receptor (EGFR)/AKT/matrix metallopeptidase (MMP) -9 pathway.…”