Matrine inhibits the invasive properties of human osteosarcoma cells by downregulating the ERK-NF-κB pathway

Li, Yi; Zhang, Zhen-Ni; Zhao, Hongmou; Zhichao, Tong; Yang, Jie; Wang, Hu; Liang, Xiaojun

doi:10.1097/cad.0000000000000136

Cited by 25 publications

(27 citation statements)

References 36 publications

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“…Matrine exerts excellent pharmacological activity and is beneficial for lowering body temperature, promoting diuresis, detoxifying, treating jaundice and preventing liver fibrosis. Previous studies indicated that matrine may be used as an effective treatment for various types of cancer, including liver (21) and lung cancer (22), gastric (23) and colon cancer (24), cervical cancer (25), leukemia (26), osteosarcoma (27), glioma (28) and nasopharyngeal carcinoma (29), and exhibits potent activity against various other types of cancer. Lung cancer occurs in the bronchial epithelium (30) and is characterized by a high morbidity rate (31), a considerable degree of malignancy, a great metastatic propensity in the early stages and the tendency to recur following tumor excision (32).…”

Section: Discussionmentioning

confidence: 99%

Matrine reduces the proliferation of A549 cells via the p53/p21/PCNA/eIF4E signaling pathway

Xiao

Liu

et al. 2017

Molecular Medicine Reports

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The aim of the present study was to investigate how matrine affects the proliferation of A549 human lung adenocarcinoma cells via the p53/p21/proliferating cell nuclear antigen (PCNA)/eukaryotic translation initiation factor 4E (eIF4E) signaling pathway. The effect of different concentrations of matrine on the proliferation of A549 cells was investigated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The migration of A549 cells following exposure to varied concentrations of matrine was detected using a Transwell cell migration assay. The effect of 240 mg/l matrine on the apoptotic rate of A549 cells was determined using flow cytometry. The change in the mRNA and protein expression levels of p53, p21, PCNA and eIF4E following exposure to matrine were detected using reverse transcription-quantitative polymerase chain reaction and western blotting, respectively. The increase of matrine from 60–240 mg/l led to reduced cell migration and inhibition of A549 cell proliferation. The apoptotic rate of A549 cells when treated with 240 mg/l matrine was significantly different when compared with the untreated control. The mRNA expression levels of p53 and p21 in the group treated with 240 mg/l matrine were significantly higher compared with the control group. The mRNA expression levels of PCNA and eIF4E were significantly lower in the 240 mg/l matrine-treated group compared with the control. The protein expression levels of p53 and p21 were significantly higher in the 240 mg/l matrine group compared with the control group. Treatment with 240 mg/l matrine reduced the protein expression levels of PCNA and eIF4E. Matrine also reduced the migration ability of A549 cells and inhibited their proliferation, which may be associated with the overexpression of p53 and p21, and the reduction of PCNA and eIF4E expression levels.

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Section: Discussionmentioning

confidence: 99%

Matrine reduces the proliferation of A549 cells via the p53/p21/PCNA/eIF4E signaling pathway

Xiao

Liu

et al. 2017

Molecular Medicine Reports

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“…Among MMPs, MMP-2 and MMP-9 were reported to play the most important roles for cancer invasion and metastasis (34,35). Previous studies have reported that matrine can reduce the expression of MMP-2 and MMP-9 in tumor cells (32,36,37). NF-κB, promoting expression of MMP-2 and MMP-9, has previously been reported as a downstream target of matrine in cancer cells (32,36,37).…”

Section: Discussionmentioning

confidence: 99%

Matrine suppresses invasion of castration-resistant prostate cancer cells by downregulating MMP-2/9 via NF-κB signaling pathway

Huang

et al. 2016

International Journal of Oncology

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Matrine is an alkaloid from Sophora flavescens that exhibits multiple protective effects on cancers. However, the molecular mechanisms of anti-metastatic effects of matrine on castration-resistant prostate cancer (CRPC) remain unknown. This study investigated the anti-metastatic effects of matrine on CRPC to identify the underlying mechanisms. The effects of matrine on the cell viability of DU145 and PC-3 cells were measured using MTS assay. The impact of matrine on expression levels of matrix metalloproteinase (MMP)-9, MMP-2, nuclear factor-κB (NF-κB) subunit p65 and phosphorylated p65 in cells untreated or treated with matrine were analyzed by western blotting. The inhibitory effects of matrine on cell migration and invasion were examined by Transwell assay. The impact of matrine on tumorigenesis in male Balb/c nude mice inoculated subcutaneously with cells were investigated in vivo. We found that matrine inhibited the growth of DU145 and PC3 cells time- and dose-dependently both in vitro and in vivo. Migration and invasion capabilities of cells were also suppressed by matrine. At the same time, matrine markedly reduced the expression levels of MMP-9, MMP-2 and p-p65 in both cell lines. Further experiments revealed that matrine exhibited inhibitory effects of migration and invasion of CRPC by downregulating MMP-2/9 through NF-κB pathway. Matrine inhibits invasion of CRPC by reducing levels of MMP-9 and MMP-2 through NF-κB pathway. Therefore, it may be a potential anti-metastatic therapeutic agent for CRPC.

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“…Another study reports that matrine inhibits the invasive activities of human osteosarcoma cells through down-regulation of the ERK/NF-κB pathway in intro and in vivo (19).…”

Section: Alkaloidmentioning

confidence: 99%

A map describing the association between effective components of traditional Chinese medicine and signaling pathways in cancer cells in vitro and in vivo

Xia

Chen

Zhang

et al. 2014

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Matrine inhibits the invasive properties of human osteosarcoma cells by downregulating the ERK-NF-κB pathway

Cited by 25 publications

References 36 publications

Matrine reduces the proliferation of A549 cells via the p53/p21/PCNA/eIF4E signaling pathway

Matrine reduces the proliferation of A549 cells via the p53/p21/PCNA/eIF4E signaling pathway

Matrine suppresses invasion of castration-resistant prostate cancer cells by downregulating MMP-2/9 via NF-κB signaling pathway

A map describing the association between effective components of traditional Chinese medicine and signaling pathways in cancer cells in vitro and in vivo

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