Matrine induces apoptosis via targeting CCR7 and enhances the effect of anticancer drugs in non-small cell lung cancer <i>in vitro</i>

Pu, Jiangtao; Tang, Xiaojun; Zhuang, Xiang; Zhang, Hu; He, Kaiming; Wu, Yingxi; Dai, Tianyang

doi:10.1177/1753425918800555

Cited by 21 publications

(18 citation statements)

References 23 publications

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“…22 Not surprisingly, Matrine promoted apoptosis via affecting apoptosis-related protein expression, including Bcl-2 and Caspase3; importantly, Matrine could inhibit drug-resistance of NSCLC cells for chemotherapeutic drugs with a dosage-dependent manner, implying the anti-cancer potentials of Matrine in NSCLC. 23 Analogous tumor inhibition impacts by Matrine in NSCLC were also confirmed by other researchers. 24,25 As we all know, it was widely reported that circRNAs exhibited their effects through functioning as miRNA sponges through competing endogenous RNAs network.…”

Section: Discussionsupporting

confidence: 59%

Matrine Regulates Proliferation, Apoptosis, Cell Cycle, Migration, and Invasion of Non-Small Cell Lung Cancer Cells Through the circFUT8/miR-944/YES1 Axis

Zhu¹,

Lu²,

Lü³

et al. 2021

CMAR

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Background: Non-small cell lung carcinoma (NSCLC) is the major histological subtype of cancer cases. In the present study, we investigated the association between Matrine, an active component of Chinese medicine, and circFUT8 in NSCLC cells. Methods: The proliferation ability of NSCLC cells was assessed by MTT and colonyforming assays. Flow cytometry assay was performed to show the apoptosis and cell cycle distribution in NSCLC cells. The protein expression levels of Bcl-2, Cleaved Caspase-3 (C-Caspase3), and YES proto-oncogene 1 (YES1) were measured by Western blot assay. Migration and invasion of NSCLC cells were determined by transwell assay. The expression levels of circFUT8, miR-944 and YES1 were quantified by real-time quantitative polymerase chain reaction (RT-qPCR) assay. The interaction relationship between miR-944 and circFUT8 or YES1 was confirmed by dual-luciferase reporter assay. The anti-tumor role of Matrine in vivo was explored by a xenograft experiment. Results: Matrine functioned as a carcinoma inhibitor by repressing proliferation, cell cycle process, migration, and invasion while inducing apoptosis in NSCLC cells. Importantly, overexpression of circFUT8 counteracted Matrine-induced effects on NSCLC cells. MiR-944, interacted with YES1, was a target of circFUT8. Under Matrine condition, overexpression of circFUT8 increased proliferation, migration, and invasion while inhibited apoptosis, which was abolished by the upregulation of miR-944. Whereas the silencing of YES1 counteracted miR-944 inhibitor-induced effects on NSCLC cells. Eventually, we also confirmed that Matrine impeded NSCLC tumor growth in vivo. Conclusion: Matrine regulated proliferation, apoptosis, cell cycle, migration, and invasion of NSCLC cells through the circFUT8/miR-944/YES1 axis, which provided novel information for Matrine in NSCLC.

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Section: Discussionsupporting

confidence: 59%

Matrine Regulates Proliferation, Apoptosis, Cell Cycle, Migration, and Invasion of Non-Small Cell Lung Cancer Cells Through the circFUT8/miR-944/YES1 Axis

Zhu¹,

Lu²,

Lü³

et al. 2021

CMAR

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“…Drug combination therapy is therefore a common and efficient strategy for cancer treatment. Matrine, an active component of Sophora flavescens, has antitumor effects in breast, ovarian, non-small cell lung and colorectal cancers, similarly to that of cisplatin in liver cancer (17)(18)(19). A study by Li et al (20) reported that treatment with matrine in combination with docetaxel inhibits the proliferation and migration of tumor cells in androgen-resistant prostate cancer, indicating the potential antitumor effects of matrine in combination with chemotherapeutic drugs.…”

Section: Discussionmentioning

confidence: 99%

Effects of matrine in combination with cisplatin on liver cancer

Cao

Deng

et al. 2020

Oncol Lett

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Matrine, an alkaloid isolated from Sophora flavescens, promotes tumor cell apoptosis and strengthens the anticancer capacity of chemotherapeutic drugs. The present study aimed to investigate the inhibitory effect and underlying mechanism of matrine in combination with cisplatin on liver cancer progression. Tumor progression was studied in nude mice. The human liver cancer cell line HepG2 was injected into BALB/c nude mice subcutaneously to establish a tumor model. Mice were subsequently treated with matrine, cisplatin, matrine + cisplatin or normal saline. Nude mice and tumor growth were monitored. Tumors were excised and the expression of survivin, caspase-3, caspase-7 and caspase-9 was detected by immunohistochemistry. Western blotting was used to determine the expression of survivin, caspase-3, caspase-7, caspase-9 and X-linked inhibitor of apoptosis protein (XIAP) in tumor tissues. The results demonstrated that matrine exerted anticancer effects in liver cancer-transplanted tumors, as evidenced by decrease in tumor weight and volume. Furthermore, the tumor inhibition rate in mice treated with matrine + cisplatin was 83.3%, whereas it was of 37.5 and 75% in mice treated with matrine or cisplatin alone, respectively. In addition, the expression of survivin and XIAP was significantly downregulated, whereas the expression of caspase-3, caspase-7 and caspase-9 was significantly upregulated in tumor tissues from nude mice treated with matrine + cisplatin, compared with those treated with cisplatin, matrine or normal saline. These findings suggested that the combination of matrine and cisplatin may promote tumor cell apoptosis in liver cancer by activating the caspase apoptosis pathway and suppressing the survivin-associated inhibition of caspase-9.

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“…Recently, enhancement of efficacy and/or toxicity due to drugdrug interactions has been a key consideration in the development of new drugs in pre-clinical and clinical investigations (Pai et al, 2018). Pu et al showed that MT combined with cisplatin, 5fluorouracil, and paclitaxel, respectively, effectively inhibited the proliferation of A549 cells and showed dose-dependent anticancer effects (Pu et al, 2018). It has been reported that a combination of MT and cisplatin synergistically inhibited proliferation, and induced apoptosis of rhabdomyosarcoma (RMS) RD cells, through a mechanism involving inhibition of mRNA expression of XIAP mRNA .…”

Section: Drug-drug Interactions Of Mtmentioning

confidence: 99%

“…Pu et al. showed that MT combined with cisplatin, 5-fluorouracil, and paclitaxel, respectively, effectively inhibited the proliferation of A549 cells and showed dose-dependent anticancer effects ( Pu et al., 2018 ). It has been reported that a combination of MT and cisplatin synergistically inhibited proliferation, and induced apoptosis of rhabdomyosarcoma (RMS) RD cells, through a mechanism involving inhibition of mRNA expression of XIAP mRNA ( Li et al., 2016 ).…”

Section: Drug-drug Interactions Of Mtmentioning

confidence: 99%

A Systematic Review of the Pharmacology, Toxicology and Pharmacokinetics of Matrine

You

Yang

et al. 2020

Front. Pharmacol.

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Matrine (MT) is a naturally occurring alkaloid and an bioactive component of Chinese herbs, such as Sophora flavescens and Radix Sophorae tonkinensis. Emerging evidence suggests that MT possesses anti-cancer, anti-inflammatory, anti-oxidant, antiviral, antimicrobial, anti-fibrotic, anti-allergic, antinociceptive, hepatoprotective, cardioprotective, and neuroprotective properties. These pharmacological properties form the foundation for its application in the treatment of various diseases, such as multiple types of cancers, hepatitis, skin diseases, allergic asthma, diabetic cardiomyopathy, pain, Alzheimer's disease (AD), Parkinson's disease (PD), and central nervous system (CNS) inflammation. However, an increasing number of published studies indicate that MT has serious adverse effects, the most obvious being liver toxicity and neurotoxicity, which are major factors limiting its clinical use. Pharmacokinetic studies have shown that MT has low oral bioavailability and short half-life in vivo. This review summarizes the latest advances in research on the pharmacology, toxicology, and pharmacokinetics of MT, with a focus on its biological properties and mechanism of action. The review provides insight into the future of research on traditional Chinese medicine.

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Matrine induces apoptosis via targeting CCR7 and enhances the effect of anticancer drugs in non-small cell lung cancer in vitro

Cited by 21 publications

References 23 publications

Matrine Regulates Proliferation, Apoptosis, Cell Cycle, Migration, and Invasion of Non-Small Cell Lung Cancer Cells Through the circFUT8/miR-944/YES1 Axis

Matrine Regulates Proliferation, Apoptosis, Cell Cycle, Migration, and Invasion of Non-Small Cell Lung Cancer Cells Through the circFUT8/miR-944/YES1 Axis

Effects of matrine in combination with cisplatin on liver cancer

A Systematic Review of the Pharmacology, Toxicology and Pharmacokinetics of Matrine

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