Matrine induces apoptosis in gastric carcinoma cells via alteration of Fas/FasL and activation of caspase-3

Dai, Zhijun; Gao, Jie; Ji, Zong-Zheng; Wang, Xijing; Ren, Hongtao; Liu, Xiaoxu; Wang, Wenying; Kang, Huafeng; Guan, Huaijin

doi:10.1016/j.jep.2009.02.022

Cited by 93 publications

(70 citation statements)

References 22 publications

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“…Most cancer cells express both FasR and FasL. The interaction between these molecules on the tumor cell surface results in the formation of a complex that initiates apoptotic signaling, leading to the activation of caspases, which in turn interacts with cytoplasmic signaling proteins that induce apoptosis (Dai et al, 2009). In this study, we observed an increase in the FasL and FasR mRNA expression after treatment with 12.5-50 mM sodium acetate in a dose-dependent manner.…”

Section: Discussionsupporting

confidence: 52%

Apoptotic effect of sodium acetate on a human gastric adenocarcinoma epithelial cell line

Yuan

Zhang

Jin³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. The objective of this study was to investigate the effect of sodium acetate on the viability of the human gastric adenocarcinoma (AGS) epithelial cell line. AGS cells were exposed to a range of concentrations of sodium acetate for different periods of time, and the sodium acetate-induced cytotoxic effects, including cell viability, DNA fragmentation, apoptotic gene expression, and caspase activity, were assessed. The changes in these phenotypes were quantified by performing a lactate dehydrogenase cell viability assay, annexin V staining, terminal deoxynucleotidyl transferase-mediated dUTP nickend labeling (TUNEL), and several caspase activity assays. In vitro studies demonstrated that the cytotoxicity of sodium acetate on the AGS cell line were dose-and time-dependent manners. No differences 2 Y. Xia et al. Genetics and Molecular Research 15 (4): gmr.15048375 were found between the negative control and sodium acetate-treated cells stained with annexin V and subjected to the TUNEL assay. However, caspase-3 activity was increased in AGS cells exposed to sodium acetate. Overall, it was concluded that sodium acetate exerted an apoptotic effect in AGS cells via a caspase-dependent apoptotic pathway.

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Section: Discussionsupporting

confidence: 52%

Apoptotic effect of sodium acetate on a human gastric adenocarcinoma epithelial cell line

Yuan

Zhang

Jin³

et al. 2016

Genet. Mol. Res.

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“…Matrine can also inhibit cell proliferation and induce apoptosis in a dose-and time-dependent manner in gastric carcinoma cells (SGC-7901). The expression of Fas/Fasl and the activity of caspase-3 enzyme increased after treatment with matrine, and these changes were highly correlated with the apoptosis rate [14] . Another study used matrine to treat human gastric cancer cells (MKN45).…”

Section: Introductionmentioning

confidence: 87%

Anti-tumor activities of active ingredients in Compound Kushen Injection

Wang¹,

et al. 2015

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Kushen (Radix Sophorae Flavescentis) has a long history of use for the treatment of tumors, inflammation and other diseases in traditional Chinese medicine. Compound Kushen Injection (CKI) is a mixture of natural compounds extracted from Kushen and Baituling (Rhizoma Smilacis Glabrae). The main principles of CKI are matrine (MT) and oxymatrine (OMT) that exhibit a variety of pharmacological activities, including anti-inflammatory, anti-allergic, anti-viral, anti-fibrotic and cardiovascular protective effects. Recent evidence shows that these compounds also produce anti-cancer actions, such as inhibiting cancer cell proliferation, inducing cell cycle arrest, accelerating apoptosis, restraining angiogenesis, inducing cell differentiation, inhibiting cancer metastasis and invasion, reversing multidrug resistance, and preventing or reducing chemotherapy-and/or radiotherapy-induced toxicity when combined with chemotherapeutic drugs. In this review, we summarize recent progress in studying the anti-cancer activities of MT, OMT and CKI and their potential molecular targets, which provide clues and references for further study.

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“…When Fas is bound to its ligand, the apoptotic signal may be transferred to the cells. Many antitumor agents induce apoptosis of tumor cells by up-regulating the expression of Fas (Okamoto et al, 2006;Kim et al, 2007;Dai et al, 2009). However, little is known about the regulation of the Fas level triggered by MTE.…”

Section: Fas Expression Of Mg63 By Immunocytochemistrymentioning

confidence: 99%

Marsdenia tenacissima extract sensitizes MG63 cells to doxorubicin-induced apoptosis

et al. 2014

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ABSTRACT. Marsdenia tenacissima extract (MTE) is a new platederived biotechnology product that is frequently used, but occasionally reported, in the field of chemotherapy. In this study, we assessed the antitumor activity and related mechanisms of MTE by various biotechnological methods. The survival rates of MG63 osteosarcoma cells treated with MTE and doxorubicin were measured, individually or jointly, and the changes in cellular shape, apoptotic rates, and Fas expression were observed. The results indicated that combination of MTE and doxorubicin up-regulated Fas expression and induced apoptosis. The survival rate of combined application of 50 mg/mL MTE and 1 µg/mL doxorubicin was significantly lower than that of the individual application (P < 0.01). Other biotechnology methods also showed an apoptosis-inducing effect of combined application that was much stronger than individual application. All of these results suggested that MTE may promote the effects of doxorubicin chemotherapy, perhaps related to the up-regulation of Fas expression in tumor cells.

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Matrine induces apoptosis in gastric carcinoma cells via alteration of Fas/FasL and activation of caspase-3

Cited by 93 publications

References 22 publications

Apoptotic effect of sodium acetate on a human gastric adenocarcinoma epithelial cell line

Apoptotic effect of sodium acetate on a human gastric adenocarcinoma epithelial cell line

Anti-tumor activities of active ingredients in Compound Kushen Injection

Marsdenia tenacissima extract sensitizes MG63 cells to doxorubicin-induced apoptosis

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