Matrine-imprinted monolithic stationary phase for extraction and purification of matrine from Sophorae flavescentis Ait

Fu, Qiang; Fang, Qi; Feng, Bianling; Sun, Shih‐Jieh; Du, Wei; Amut, Enijian; Xiao, Aiping; Chang, Chun

doi:10.1016/j.jchromb.2011.02.041

Cited by 29 publications

(14 citation statements)

References 23 publications

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“…In this study, we selected the matrine and oxymatrine as template molecule to prepare the double‐templated molecularly imprinted polymers. The functional monomer was selected according to UV‐Vis spectra, and the cross linker and initiator were selected according to a previous report . Electrostatic interactions, hydrogen bonding, π–π interactions, and hydrophobic interactions may be responsible for formation of the binding sites in MIPs, and the selectivity and specificity arise from these binding sites .…”

Section: Resultsmentioning

confidence: 99%

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Extraction and identification of matrine‐type alkaloids from Sophora moorcroftiana using double‐templated molecularly imprinted polymers with HPLC–MS/MS

Lin

Zhang

et al. 2018

J of Separation Science

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Double-templated molecularly imprinted polymers with specific recognition of three matrine-type alkaloids were prepared using matrine and oxymatrine as the template molecules. An approach based on double-templated molecularly imprinted solid-phase extraction coupled with high-performance liquid chromatography and tandem mass spectrometry was then developed to extract and purify matrine, oxymatrine, and sophocarpine from Sophora moorcroftiana in the Tibetan plateau herbs. The polymers were characterized by Fourier-transform infrared spectroscopy and scanning electron microscopy. Their adsorption characteristics were evaluated using adsorption kinetics, isotherms, selectivity, and recycling experiments. This polymer exhibited excellent molecular recognition ability and good selectivity. The obtained polymers as adsorbent was further used for the determination of three matrine-type alkaloids coupled to high-performance liquid chromatography with tandem mass spectrometry, the recoveries of three matrines spiked at three concentration levels in samples were 73.25-98.42% (n = 5) with a relative standard deviation less than 6.82%. The limits of detection for the method were 9.23-15.42 μg/kg (S/N = 3). This proposed method was assessed to be an effective method for simultaneous extraction, isolation, and identification of matrine, oxymatrine, and sophocarpine from Sophora moorcroftiana.

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Section: Resultsmentioning

confidence: 99%

“…For subsequent experiments, we selected a ratio of two templates to the functional monomer of 1:3. EGDMA was chosen in this work because it is the most widely used as the cross‐linker, and relational application was also found in the matrine‐imprinted monolithic stationary phase .…”

Section: Resultsmentioning

confidence: 99%

Extraction and identification of matrine‐type alkaloids from Sophora moorcroftiana using double‐templated molecularly imprinted polymers with HPLC–MS/MS

Lin

Zhang

et al. 2018

J of Separation Science

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“…Compared with conventional extraction techniques, molecularly imprinted solid-phase extraction (MISPE) is an effective method for enrichment and separation of bioactive molecules (21)(22)(23). This method uses a MIP as the column packing to effectively avoid matrix interference from complex mixtures (24,25). Although many methods based on molecular imprinting have been reported for the extraction of active components, and some of these have achieved good results for flavonoids and alkaloids (19), there are issues with false positive results and template leakage.…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of dummy molecularly imprinted polymers for separation and determination of farrerol from Rhododendron aganniphum using HPLC

Lin

Zhang

et al. 2018

Green Chemistry Letters and Reviews

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A dummy molecularly imprinted polymer (d-MIP) was developed for extraction and cleanup of farrerol from Rhododendron aganniphum. The d-MIP was prepared by precipitation polymerization using quercetin as the dummy template, 4-vinylpyridine as the functional monomer, ethylene glycol dimethacrylate as the cross-linker, and acetonitrile as the porogen. The d-MIP was characterized by scanning electron microscopy and Fourier-transform infrared spectroscopy. Dynamic curves, adsorption isotherms, and Scatchard plots showed the d-MIP had good affinity, capacity, and selectivity for the target compound. The prepared d-MIP was used as a sorbent in solid-phase extraction cartridges and successfully applied to specific extraction and purification of farrerol from real samples. High recoveries were obtained for four spiked levels of farrerol in Rhododendron aganniphum using d-MIP solid-phase extraction coupled with high-performance liquid chromatography. The linear range was 1-100 µg mL −1 , the limit of detection was 2.23 mg kg −1 , the farrerol recovery range was 85.7%-104.1%, and the relative standard deviations (n = 3) were all less than 2.2% for real samples. The proposed method provides a simple, rapid, and environmentally friendly approach to the analysis of farrerol in complex herbal products.

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“…MIPs are widely used in various fields of biological analysis , residue detection , and impurities removal . MIPs have numerous advantages such as high‐recognition ability, good mechanical stability, and reusability . Due to the high selectivity and stability of MIPs, it has been widely used as a new selective adsorbent for SPE for direct extraction of target compound from TCM samples .…”

Section: Introductionmentioning

confidence: 99%

Selective analysis of aristolochic acid I in herbal medicines by dummy molecularly imprinted solid‐phase extraction and HPLC

Guo

et al. 2017

J of Separation Science

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In this study, surface molecularly imprinted polymers were prepared as the selective sorbents for separation of aristolochic acid I in herbal medicine extracts by a facile approach. A less toxic dummy template, ofloxacin, was used to create specific molecule recognition sites for aristolochic acid I in the synthesized polymers. The polymers were characterized by Fourier-transfer infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, elemental analysis, and nitrogen adsorption-desorption test. The adsorption capacity was calculated using adsorption kinetics, selectivity, and recycling experiments. The obtained polymers exhibited high thermostability, fast equilibrium time, and excellent binding ability. Subsequently, the polymers applied as the solid-phase extraction absorbent was proposed and used for the enrichment and analysis of aristolochic acid I in herbal plants. The result showed that the aristolochic acid I was enriched up to 16 times after analysis by using high-performance liquid chromatography. The good linearity for aristolochic acid I was obtained in the range of 0.1-200 μg/mL (R = 0.9987). The recovery and precision values were obtained (64.94-77.73%, RSDs% ≤ 0.8%, n = 3) at three spiked concentration levels. This work provided a promising method for selective enrichment, extraction, and purification of aristolochic acid I from complex herbal plants.

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Matrine-imprinted monolithic stationary phase for extraction and purification of matrine from Sophorae flavescentis Ait

Cited by 29 publications

References 23 publications

Extraction and identification of matrine‐type alkaloids from Sophora moorcroftiana using double‐templated molecularly imprinted polymers with HPLC–MS/MS

Extraction and identification of matrine‐type alkaloids from Sophora moorcroftiana using double‐templated molecularly imprinted polymers with HPLC–MS/MS

Preparation and characterization of dummy molecularly imprinted polymers for separation and determination of farrerol from Rhododendron aganniphum using HPLC

Selective analysis of aristolochic acid I in herbal medicines by dummy molecularly imprinted solid‐phase extraction and HPLC

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