Matrilysin Expression in the Involuting Rat Ventral Prostate

Powell, William C.; Domann, Frederick E.; Mitchen, Joan M.; Matrisian, Lynn M.; Nagle, Raymond B.; Bowden, G. Tim

doi:10.1002/1097-0045(199609)29:3<159::aid-pros2990290304>3.0.co;2-l

Cited by 42 publications

(38 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Moreover, the results presented in the present study perfectly matched those obtained by Powell et al (1996) using in situ hybridization for MMP-7 mRNA during involution of the rat ventral prostate. Those authors showed individual portions of the ducts or small clusters of epithelial cells expressing high levels of matrilysin, with adjacent ducts frequently negative.…”

Section: Discussionsupporting

confidence: 88%

Epithelial‐stromal transition of MMP‐7 immunolocalization in the rat ventral prostate following bilateral orchiectomy

Felisbino¹,

Justulin²,

Carvalho³

et al. 2007

Cell Biology International

View full text Add to dashboard Cite

Epithelial cells from involuting rat ventral prostate (VP) express Matrilysin (MMP-7) mRNA. Herein, we investigated by immunohistochemistry the MMP-7 protein location and its association with tissue changes following castration in the VP. Normal and castrated adult male Wistar rats were sacrificed at different times after surgery. VP was examined by immunocytochemistry and immunoprecipitation. Castration promoted a shrinking of prostate ducts with an extensive stromal remodeling. In the VP from normal rats, MMP-7 immunoreactivity was found in epithelial secretory granules. Three days after castration, immunostaining for MMP-7 was found in both the epithelial secretory granules and in the stroma just below the epithelium, mainly at the distal ductal tips. At seven and 21 days after castration, the immunostaining for MMP-7 was found only in the stromal space. Immunoprecipitation confirmed the specificity of the primary antibody by rescuing a pro-enzyme form (28kDa) in the prostate extracts. The present results suggest that MMP-7 participates in the epithelial-stromal interface remodeling of the ventral prostate during the involution achieved by castration, probably in the degradation of components of the epithelial basement membrane.

show abstract

Section: Discussionsupporting

confidence: 88%

Epithelial‐stromal transition of MMP‐7 immunolocalization in the rat ventral prostate following bilateral orchiectomy

Felisbino¹,

Justulin²,

Carvalho³

et al. 2007

Cell Biology International

View full text Add to dashboard Cite

show abstract

“…Patients with ductal metaplasia arising in the setting of chronic pancreatitis have a 16-fold increase in relative risk for pancreatic ductal adenocarcinoma (PDAC) (Lowenfels et al, 2000). MMP7 is expressed in the epithelium of pre-malignant lesions in multiple glandular tissues, including breast, prostate, and intestine (Powell et al, 1996) and is thought to influence the initiation and maintenance of metaplastic events in the pancreatic epithelium (Crawford et al, 2002). We detected elevated MMP7 levels in HNF6 Δpanc animals.…”

Section: Discussionmentioning

confidence: 99%

Multiple, temporal-specific roles for HNF6 in pancreatic endocrine and ductal differentiation

Zhang

Ables

Pope

et al. 2009

Mechanisms of Development

109

View full text Add to dashboard Cite

Within the developing pancreas Hepatic Nuclear Factor 6 (HNF6) directly activates the pro-endocrine transcription factor, Ngn3. HNF6 and Ngn3 are each essential for endocrine differentiation and HNF6 is also required for embryonic duct development. Most HNF6−/− animals die as neonates, making it difficult to study later aspects of HNF6 function. Here, we describe, using conditional gene inactivation, that HNF6 has specific functions at different developmental stages in different pancreatic lineages. Loss of HNF6 from Ngn3-expressing cells (HNF6Δendo) resulted in fewer multipotent progenitor cells entering the endocrine lineage, but had no effect on β cell terminal differentiation. Early, pancreas-wide HNF6 inactivation (HNF6Δpanc) resulted in endocrine and ductal defects similar to those described for HNF6 global inactivation. However, all HNF6Δpanc animals survived to adulthood. HNF6Δpanc pancreata displayed increased ductal cell proliferation and metaplasia, as well as characteristics of pancreatitis, including up-regulation of CTGF, MMP7, and p8/Nupr1. Pancreatitis was most likely caused by defects in ductal primary cilia. In addition, expression of Prox1, a known regulator of pancreas development, was decreased in HNF6Δpanc pancreata. These data confirm that HNF6 has both early and late functions in the developing pancreas and is essential for maintenance of Ngn3 expression and proper pancreatic duct morphology.

show abstract

“…8), and also their loss of activity at day 15 postcastration indicates that these activated proteases may be related to the apoptosis or prostatic involution. Activity of proteases, including cathepsin D (Helminen et al 1972;Chiang et al 1982;Tanabe et al 1982), metalloproteases (Wilson et al 1991(Wilson et al , 1995Powell et al 1996), and plasminogen activators (Rennie et al 1984;Wilson et al 1996) have been demonstrated in the castrated rat prostates and their elevated activities are believed to be involved in the prostatic involution. Among the activated proteases, Wilson et al (1991) have identified three Ca 2+ -independent proteases (35, 73-76 kDa) and another three Ca 2+ -dependent proteases (58, 63, 66 kDa) expressed in the castrated ventral prostate.…”

Section: Fig 10mentioning

confidence: 99%

Ultrastructural and biochemical observations on the early changes in apoptotic epithelial cells of the rat prostate induced by castration

et al. 1999

View full text Add to dashboard Cite

The present study describes the sequential ultrastructural changes in the apoptotic cells of the rat ventral and dorsal prostates during the early period of 1-3 days postcastration. The major morphological changes include: (1) condensation of heterochromatin along the nuclear envelope and fragmentation into crescent-shaped micronuclei; (2) formation of membrane-bound cytoplasmic spherical bodies, which contain various organelles and micronuclei, within the apoptotic cells; (3) formation of non-membrane-bound autolytic vacuoles by autolysis of cytoplasm; (4) focal rupture of outer mitochondrial membrane; and (5) phagocytosis of the fragmented cytoplasmic spherical bodies and apoptotic cells by macrophages. The occurrence of both cytoplasmic apoptotic bodies and autolytic vacuoles in apoptotic cells suggests that the cytoplasm of the apoptotic cells could be destroyed by different means. The responsiveness of different prostatic lobes to androgen withdrawal and the time course of the transitory apoptotic activity in different lobes were analyzed by counting the indices of the TUNEL-labeled apoptotic cells against the postcastration periods. The results showed that the ventral lobe responded more rapidly to castration than the lateral and dorsal lobes. The dorsal lobe was the slowest in response to castration among the three lobes. Analysis of protease activities by zymography has identified two Ca(2+)-independent proteases of apparent MW 20 and 24 kDa (expressed in both ventral and dorsolateral lobes), and one Ca(2+)-dependent protease of MW 66.5 kDa (expressed only in the dorsolateral lobe) which became activated at day 3 postcastration. Their expression patterns were different from that of CPP-3 in the castrated prostates, suggesting that the activated proteases were enzymes other than CPP-3. The association of their highest activities with the maximum apoptotic activity at day 3 postcastration and also their loss of activity at day 15 suggest that these protease activities might be related to apoptosis or glandular involution.

show abstract

Matrilysin Expression in the Involuting Rat Ventral Prostate

Cited by 42 publications

References 52 publications

Epithelial‐stromal transition of MMP‐7 immunolocalization in the rat ventral prostate following bilateral orchiectomy

Epithelial‐stromal transition of MMP‐7 immunolocalization in the rat ventral prostate following bilateral orchiectomy

Multiple, temporal-specific roles for HNF6 in pancreatic endocrine and ductal differentiation

Ultrastructural and biochemical observations on the early changes in apoptotic epithelial cells of the rat prostate induced by castration

Contact Info

Product

Resources

About