matK DNA Sequences and Phylogenetic Reconstruction in Saxifragaceae s. str.

Johnson, Leigh A.; Soltis, Douglas E.

doi:10.2307/2419718

Cited by 486 publications

(265 citation statements)

References 37 publications

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“…Double-stranded DNAs were amplified by using PCR to amplify the ITS-1, ITS-2, and 5.8S region of nuclear ribosomal DNA using the ITS-4 and ITS-5 primers (23). The cpDNA trnK introns and matK coding region were amplified by using the primers trnK-3914F and trnK-2R (24). PCR products were cloned into plasmids by using a TOPO TA cloning kit (Invitrogen).…”

Section: Methodsmentioning

confidence: 99%

Evidence of hybridity in invasive watermilfoil ( Myriophyllum ) populations

Moody

Les

2002

Proc. Natl. Acad. Sci. U.S.A.

141

140

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Invasions of nonindigenous species have caused ecological devastation to natural communities worldwide, yet the biological bases for invasiveness remain poorly understood. Our studies of invasive watermilfoil (Myriophyllum) populations revealed widespread polymorphisms in biparentally inherited nuclear ribosomal DNA sequences, which were not detected in populations of native North American species. Subclones of the polymorphic regions revealed the occurrence of distinct sequences matching those acquired from both nonindigenous and native North American species. Molecular data demonstrate clearly that invasive watermilfoil populations in North America have resulted from hybridization between nonindigenous and native species. These observations suggest that invasiveness in these aggressive aquatic weeds may be linked to heterosis maintained by vegetative propagation.

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Section: Methodsmentioning

confidence: 99%

Evidence of hybridity in invasive watermilfoil ( Myriophyllum ) populations

Moody

Les

2002

Proc. Natl. Acad. Sci. U.S.A.

141

140

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show abstract

“…These included portions of three low-copy nuclear genes: chloroplast-expressed glutamine synthetase (ncpGS), nitrate reductase (NIA), and the fourth intron of phosphoenolpyruvate carboxylase (PepC) and eight noncoding regions of the chloroplast genome, selected for their relatively high variability in other angiosperms: six intergenic regions (petN-psbM, psbE-petL, psbM-trnD, trnD-trnT, and trnQ-rpS16); a portion of the tRNA-Lys (trnK) intron with primers in 5 0 trnK and 5 0 matK; a portion of the trnK intron with primers in 3 0 matK and 3 0 trnK; and the RPS16 intron. PCR primers were previously published (Demesure et al, 1995;Emshwiller and Doyle, 1999;Gaskin and Schaal, 2002;Howarth and Baum, 2002;Johnson and Soltis, 1994;Lee and Wen, 2004;Popp et al, 2005;Popp and Oxelman, 2001;Shaw et al, 2005Shaw et al, , 2007 or designed for this study (Supplementary Table S1). …”

Section: Dna Isolation Amplification and Sequencingmentioning

confidence: 99%

Estimating the species tree for Hawaiian Schiedea (Caryophyllaceae) from multiple loci in the presence of reticulate evolution

Willyard

Wallace

Wagner

et al. 2011

Molecular Phylogenetics and Evolution

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“…Generally, DNA extraction procedures were from silica-gel-dried leaves (Chase and Hills 1991) or herbarium sheets as summarized by Fay et al (2000). A description of the amplification procedures for the seven loci can be found in the following references: rbcL , atpB , trnL-F (primers "c" and "f" of Taber let et al 1991, which include two regions, trnL intron and trnL-F intergenic spacer), matK (Johnson and Soltis 1994), ndhF (Pires and Sytsma 2002;McPherson et al submitted), and atp 1 (Davis et al 1998Stevenson et al 2000). GenBank numbers for rbcL, atpB, and trnL-F have been previously published Pireset al 2004).…”

Section: Dna Extraction Gene Amplification Sequencing and Alignmentmentioning

confidence: 99%

Phylogeny, Genome Size, and Chromosome Evolution of Asparagales

Pires¹,

Maureira²,

Givnish³

et al. 2006

aliso

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Asparagales are a diverse monophyletic order that has numerous species (ca. 50% of monocots) including important crop plants such as Allium, Asparagus, and Vanilla, and a host of ornamentals such as irises, hyacinths, and orchids. Historically, Asparagales have been of interest partly because of their fascinating chromosomal evolution. We examine the evolutionary dynamics of Asparagales genomes in an updated phylogenetic framework that combines analyses of seven gene regions (atpl, atpB, matK, ndhF, rbcL, trnL intron, and trnL-F intergenic spacer) for 79 taxa of Asparagales and outgroups. Asparagales genomes are evolutionarily labile for many characters, including chromosome number and genome size. The history and causes of variation in chromosome number and genome size remain unclear, primarily because of the lack of data in small clades in the phylogenetic tree and the lack of comparative genetic maps, apart from Allium and Asparagus. Genomic tools such as bacterial artificial chromosome (BAC) libraries should be developed, as both molecular cytogenetic markers and a source of nuclear genes that can be widely used by evolutionary biologists and plant breeders alike to decipher mechanisms of chromosomal evolution.

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matK DNA Sequences and Phylogenetic Reconstruction in Saxifragaceae s. str.

Cited by 486 publications

References 37 publications

Evidence of hybridity in invasive watermilfoil ( Myriophyllum ) populations

Evidence of hybridity in invasive watermilfoil ( Myriophyllum ) populations

Estimating the species tree for Hawaiian Schiedea (Caryophyllaceae) from multiple loci in the presence of reticulate evolution

Phylogeny, Genome Size, and Chromosome Evolution of Asparagales

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