Mating Types in Tetrahymena

Elliott, Alfred M.; Hayes, Robert E.

doi:10.2307/1538642

Cited by 133 publications

(68 citation statements)

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“…Thymidine- 3 H is incorporated into a mitochondrial DNA which is conserved during four generations of logarithmic growth (14,15). The mitochondrial localization and stability of this DNA has been confirmed by Stone and Miller (22).…”

Section: Introductionmentioning

confidence: 85%

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THE DISTRIBUTION OF DNA AMONG DIVIDING MITOCHONDRIA OF TETRAHYMENA PYRIFORMIS

Parsons¹,

Rustad²

1968

The Journal of Cell Biology

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A squash technique was developed for log phase Tetrahymena pyriformis which permitted the resolution of over 100 individual mitochondria from a single cell. Mitochondria incorporated thymidine at all stages of the cell cycle, even when nuclear DNA synthesis was not occurring. During the stage of macronuclear DNA synthesis, however, there was a significant increase in the extent of mitochondrial labeling. Low radioautograph background suggests that mitochondrial DNA is synthesized at the mitochondria themselves. All mitochondria incorporated thymidine-3H within one population-doubling time. Grain counts also showed that the amount of mitochondrial label was retained for four generations and that this label remained randomly distributed among all mitochondria during this time. The results are not consistent with any theory of de-novo or "microbody" origin of mitochondria, but do support the hypothesis that mitochondria are produced by the growth and division of preexisting mitochondria. The stability of the mitochondrial DNA and its distribution among daughter mitochondria satisfy two prerequisites for a genetic material. The possibility is discussed that some of the genetic information for the mitochondrion is contained in the DNA associated with this organelle.

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Section: Introductionmentioning

confidence: 85%

“…All radioactive labeling was done in a chemically defined medium modified from Elliott and Hayes (3). In addition to the regular ingredients, the defined medium contained 400 mg/liter proteose-peptone and was prepared with half the pyrimidine content listed by Elliott and Hayes or with no pyrimidines added.…”

Section: Culture Conditionsmentioning

confidence: 99%

THE DISTRIBUTION OF DNA AMONG DIVIDING MITOCHONDRIA OF TETRAHYMENA PYRIFORMIS

Parsons¹,

Rustad²

1968

The Journal of Cell Biology

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“…They were grown axenically for 3 days (log growth 1 Strains A15651c and B16652e, mating types I and II, respectively, were provided through the courtesy of Dr. Sally Allen, Department of Zoology, University of Michigan, Ann Arbor, Mich. phase) in 500-ml Erlenmeyer flasks containing 100 ml of modified peptone medium (6). When starvation was under investigation, the ciliates were axenically washed and resuspended in a sterile inorganic buffer solution (4).…”

Section: Methodsmentioning

confidence: 99%

“…Whether or not they have common origins and functions is impossible to determine from ultrastructural evidence. Conjugation is induced when ciliates are subjected to a period of starvation (6). During this period, the isolated saccules noted in growing cells appear to become aligned into two or more lamellae.…”

Section: Figurementioning

confidence: 99%

A GOLGI APPARATUS ASSOCIATED WITH MATING IN TETRAHYMENA PYRIFORMIS

Elliott

Zieg

1968

The Journal of Cell Biology

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The classical Golgi apparatus has not been observed in the several strains of Tetrahymena pyriformis examined in this laboratory at the ultrastructural level when the ciliates are grown vegetatively. However, sexually active strains, when starved for the purpose of inducing conjugation, contain stacked saccules in the oral region. When such opposite mating types are mixed for mating, the stacked saccules become swollen at their ends and vesicles appear to pinch off from them. These bodies possess the configuration of the classical Golgi apparatus of other eucells. Vesicles seem to be formed from the saccules just prior to, and toward the end of conjugation, suggesting a relationship with the mating process.

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“…However, under conditions of starvation, pairs of sexually mature cells with different mating types undergo conjugation. T. thermophila cells can have one of seven different mating types, and each mating type can conjugate with another mating type, but not with itself (Elliott and Gruchy 1952;Nanney and Caughey 1953). Conjugation involves highly conserved eukaryotic processes: meiosis, gametogenesis, and gamete nucleus fusion to form the fertilization (or "zygote") nuclei of progeny cells (Figure 3).…”

Section: Tetrahymena Biologymentioning

confidence: 99%

Tetrahymenaas a Unicellular Model Eukaryote: Genetic and Genomic Tools

2016

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Tetrahymena thermophila is a ciliate model organism whose study has led to important discoveries and insights into both conserved and divergent biological processes. In this review, we describe the tools for the use of Tetrahymena as a model eukaryote, including an overview of its life cycle, orientation to its evolutionary roots, and methodological approaches to forward and reverse genetics. Recent genomic tools have expanded Tetrahymena's utility as a genetic model system. With the unique advantages that Tetrahymena provide, we argue that it will continue to be a model organism of choice.

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Mating Types in Tetrahymena

Abstract: The potentialities of Tetrahymcna as a genetic tool have long been considered by those working with this ciliated protozoan. Its value stems from the fact that it

Cited by 133 publications

References 10 publications

THE DISTRIBUTION OF DNA AMONG DIVIDING MITOCHONDRIA OF TETRAHYMENA PYRIFORMIS

THE DISTRIBUTION OF DNA AMONG DIVIDING MITOCHONDRIA OF TETRAHYMENA PYRIFORMIS

A GOLGI APPARATUS ASSOCIATED WITH MATING IN TETRAHYMENA PYRIFORMIS

Tetrahymenaas a Unicellular Model Eukaryote: Genetic and Genomic Tools

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