Mating triggers immediate upregulation of the heat shock response but downregulation of immune activity in the female reproductive tract of moths

Wang, Fen; Gao, Bo; Yu, Hong; Yong-he, LI; Chen, Peng; Xu, Jin

doi:10.1016/j.aspen.2022.101919

Cited by 1 publication

(4 citation statements)

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“…Previous studies generally found that mating induces the upregulation of egg production and fertilization rate [ 32 , 35 , 36 , 41 ], whereas in C. chuxiongica, mating induced divergent reproductive response, with downregulation on genes related to egg production, while upregulation on genes related to egg fertilization [ 38 ]. In the present study, functional enrichment analysis in S. frugiperda females showed there is more reproduction related terms/pathways enriched to upregulated DEGs than to downregulated DEGs (10:1) at 0 h postmating, which is less (1:14) at 6 h postmating, and then more (16:0) again at 24 h postmating.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have shown that mating may also promote trade-offs between reproduction and survival, with increased reproductive activities after mating being likely to incur cost on soma maintenance [ 36 , 37 , 38 , 41 ]. In the present study, however, mating induced upregulation on response (such as response to external biotic stimulus, response to bacterium and response to external stimulus; Tables S8–S11 ) and soma maintenance (such as immune activity, stress reaction and detoxification; Tables S8–S11 ) related processes in S. frugiperda females at 0, 6 and 24 h postmating ( Figure 5 a–c).…”

Section: Discussionmentioning

confidence: 99%

“…Mating can challenge the female’s immune system by transferring foreign materials and infections to females [ 77 , 78 , 79 , 80 ]; females of polyandrous species should have higher postmating immunity if sexually transmitted infection is the major factor driving female postmating costs [ 80 ]. Therefore, mating-induced transcriptional changes should be sex-specific (this study) and species- and tissue-specific [ 36 , 41 ].…”

Section: Discussionmentioning

confidence: 99%

“…Studies in Drosophila [ 32 , 33 , 34 , 35 ] and other insect species [ 36 , 37 , 38 ] demonstrated that mating can cause significant transcriptional changes in females [ 32 , 33 , 34 , 35 ], which may support them to obtain a high rate of fertilization and fecundity. These studies on different insect species, based on depth sequencing and bioinformatics, have provided further insights in this field.…”

Section: Introductionmentioning

confidence: 99%

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Mating-Induced Common and Sex-Specific Behavioral, Transcriptional Changes in the Moth Fall Armyworm (Spodoptera frugiperda, Noctuidae, Lepidoptera) in Laboratory

Cao

Liu

et al. 2023

Insects

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The intermediate process between mating and postmating behavioral changes in insects is still poorly known. Here, we studied mating-induced common and sex-specific behavioral and transcriptional changes in both sexes of Spodoptera frugiperda and tested whether the transcriptional changes are linked to postmating behavioral changes in each sex. A behavioral study showed that mating caused a temporary suppression of female calling and male courting behavior, and females did not lay eggs until the next day after the first mating. The significant differences on daily fecundity under the presence of males or not, and the same or novel males, suggest that females may intentionally retain eggs to be fertilized by novel males or to be fertilized competitively by different males. RNA sequencing in females revealed that there are more reproduction related GO (gene ontology) terms and KEGG (Kyoto encyclopedia of genes and genomes) pathways (mainly related to egg and zygote development) enriched to upregulated DEGs (differentially expressed genes) than to downregulated DEGs at 0 and 24 h postmating. In males, however, mating induced DEGs did not enrich any reproduction related terms/pathways, which may be because male reproductive bioinformatics is relatively limited in moths. Mating also induced upregulation on soma maintenance (such as immune activity and stress reaction) related processes in females at 0, 6 and 24 h postmating. In males, mating also induced upregulation on soma maintenance related processes at 0 h postmating, but induced downregulation on these processes at 6 and 24 h postmating. In conclusion, this study demonstrated that mating induced sex-specific postmating behavioral and transcriptional changes in both sexes of S. frugiperda and suggested that the transcriptional changes are correlated with postmating physiological and behavioral changes in each sex.

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