Mating of 2 Laboratory <b><i>Saccharomyces cerevisiae</i></b> Strains Resulted in Enhanced Production of 2-Phenylethanol by Biotransformation of <smlcap>L</smlcap>-Phenylalanine

Abstract

2-Phenylethanol (2-PE) is an aromatic alcohol with a rosy scent which is widely used in the food, fragrance, and cosmetic industries. Promising sources of natural 2-PE are microorganisms, especially yeasts, which can produce 2-PE by biosynthesis and biotransformation. Thus, the first challenging goal in the development of biotechnological production of 2-PE is searching for highly productive yeast strains. In the present work, 5 laboratory Saccharomyces cerevisiae strains were tested for the production of 2-PE. Thereafter, 2 of them were hybridized by a mating procedure and, as a result, a new diploid, S. cerevisiae AM1-d, was selected. Within the 72-h batch culture in a medium containing 5 g/L of <smlcap>L</smlcap>-phenylalanine, AM1-d produced 3.83 g/L of 2-PE in a shaking flask. In this way, we managed to select the diploid S. cerevisiae AM1-d strain, showing a 3- and 5-fold increase in 2-PE production in comparison to parental strains. Remarkably, the enhanced production of 2-PE by the hybrid of 2 yeast laboratory strains is demonstrated here for the first time.

Screening of yeasts for the production of 2-phenylethanol (rose aroma) in organic waste-based media

“…Subsequently, isolated yeasts were tested for the production of 2‐PE in medium 8 specific for l ‐Phe biotransformation to 2‐PE (Mierzejewska et al . ) in 72 h batch cultures in shaking glass tubes. Results presented in Fig.…”

“…A 5‐mm sterile loop full of biomass from single colonies was used to inoculate 5 ml of medium 8 containing 15 g l −1 glucose, 8 g l −1 sucrose, 5 g l −1 l ‐Phe, 0·5 g l −1 MgSO 4 , 1·7 g l −1 YNB without amino acids and ammonium sulphate, pH 4·6 (Mierzejewska et al . ) or medium WGP (Table ) in 25 ml glass tubes. For experiments in 100 ml flasks, 2 ml of overnight culture in YPD was diluted in 50 ml of WGP, WMP, WJP or WSP.…”

Hydrolyzed corn stover as a promising feedstock for 2‐phenylethanol production by nonconventional yeast

“…As a control culture, 2‐PE was not added to the fourth flask. The cultivation was carried out in triplicate for 72 h and the growth was monitored by OD600 measurement, as described previously …”

The Smell of Synthetic Biology: Engineering Strategies for Aroma Compound Production in Yeast

“…Media composition, especially adding L-phenylalanine, have shown in many cases to enhance the production of polypropanoids [46,47]. This implies that the yeast precursor is still a major bottleneck for phenylpropanoid production.…”