Mating disrupts morning anticipation in Drosophila melanogaster females

Riva, Sabrina; Ispizua, Juan Ignacio; Breide, María Trinidad; Polcowñuk, Sofía; Lobera, José Ricardo; Ceriani, M. Fernanda; Risau-Gusmán, Sebastián; Franco, Diana

doi:10.1371/journal.pgen.1010258

Cited by 7 publications

(9 citation statements)

References 54 publications

(115 reference statements)

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“…This would imply that locomotor activity and oviposition rhythms are controlled by distinct groups of clock neurons. However, Bulthuis et al (2019) assessed the locomotor activity of male flies, and it is well known that, unlike males, females undergo important changes in their activity after mating, such as the loss of the midday siesta 40 or the morning anticipation 41 . Thus, we examined the influence of the molecular clock of E cells directly on the locomotor activity of mated females.…”

Section: Resultsmentioning

confidence: 99%

“…In particular, the female mating rate has been shown to display circadian rhythmicity 54 , and very recently it has been proposed that pC1b neurons are involved in the control of female copulation rate 55 . Conversely, given that the E3-pC1b connection is bidirectional, it could also be instrumental in conveying information about mating status to the circadian clock, therefore producing the changes in temporal organization that females undergo after mating 40,41,56 .…”

Section: Discussionmentioning

confidence: 99%

“…For locomotor activity experiments, these files obtained from the video tracking were processed with an analysis software we developed (in Bash) which provides statistics for activity (position, distance traveled, etc.) 41 .…”

Section: Methodsmentioning

confidence: 99%

“…The standard setup for assessing locomotor activity is the Drosophila Activity Monitoring (DAM) system 42 , where flies are housed in small glass tubes (of 5mm in diameter). Such arena does not offer an adequate environment for testing flies that lay eggs during several days; instead, we monitored activity using an alternative system developed in our lab, where flies are housed in much larger cubicles and their activity is recorded by a video system (see Methods and 41 ). Interestingly, mated females with disrupted E cell clocks were as rhythmic as control flies (Figure 5), showing that there is no sex-specific contribution of this cluster to the control of rhythmic rest-activity cycles, which opens the possibility that E cells differently contribute to the control of oviposition and locomotor activity.…”

Section: It Has Recently Been Shown That Disrupting the Molecular Clo...mentioning

confidence: 99%

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Circadian control of a sex-specific behaviour in Drosophila

Riva,

Ceriani,

Risau-Gusman

et al. 2024

Preprint

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An endogenous circadian clock controls many of the behavioral traits of Drosophila melanogaster. This clock relies on the activity of interconnected clusters of neurons that harbor the clock machinery. The hierarchy among clusters involved in the control of rest-activity cycles has been extensively studied. Sexually dimorphic behaviors, on the other hand, have received less attention. Even though egg-laying, a female characteristic behavior, has been shown to be rhythmic, it remains largely unexplored possibly due to metholodological constraints. The current study provides the first steps towards determining the neural substrates underlying the circadian control of egg-laying. We show that, whereas the lateral ventral neurons (LNvs) and the dorsal neurons (DNs) are dispensable, the lateral dorsal neurons (LNds) are necessary for rhythmic egg-laying. Systematically probing the Drosophila connectome for contacts between circadian clusters and oviposition-related neurons, we found no evidence of direct connections between LNvs or DNs and neurons recruited during oviposition. Conversely, we did find bidirectional connections between Cryptochrome (Cry) expressing LNd (Cry+ LNds) and oviposition related neurons. Taken together, these results reveal that cry positive LNd neurons have a leading role in the control of the egg-laying rhythm in Drosophila females.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: It Has Recently Been Shown That Disrupting the Molecular Clo...mentioning

confidence: 99%

See 2 more Smart Citations

Circadian control of a sex-specific behaviour in Drosophila

Riva,

Ceriani,

Risau-Gusman

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…(2019) assessed the locomotor activity of male flies, and it is well known that, unlike males, females undergo important changes in their activity after mating, such as the loss of the midday siesta 40 or the morning anticipation 41 . Thus, we examined the influence of the molecular clock of E cells directly on the locomotor activity of mated females.…”

Section: It Has Recently Been Shown That Disrupting the Molecular Clo...mentioning

confidence: 99%

Circadian control of a sex-specific behaviour in Drosophila

Franco,

Riva,

Ceriani

et al. 2024

Preprint

Self Cite

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An endogenous circadian clock controls many of the behavioral traits of Drosophila melanogaster. This ¨clock¨ relies on the activity of interconnected clusters of neurons that harbor the clock machinery. The hierarchy among clusters involved in the control of rest-activity cycles has been extensively studied. Sexually dimorphic behaviors, on the other hand, have received less attention. Even though egg-laying, a female characteristic behavior, has been shown to be rhythmic, it remains largely unexplored possibly due to metholodological constraints. The current study provides the first steps towards determining the neural substrates underlying the circadian control of egg-laying. We show that, whereas the lateral ventral neurons (LNvs) and the dorsal neurons (DNs) are dispensable, the lateral dorsal neurons (LNds) are necessary for rhythmic egg-laying. Systematically probing the Drosophila connectome for contacts between circadian clusters and oviposition-related neurons, we found no evidence of direct connections between LNvs or DNs and neurons recruited during oviposition. Conversely, we did find bidirectional connections between Cryptochrome (Cry) expressing LNd (Cry+ LNds) and oviposition related neurons. Taken together, these results reveal that cry positive LNd neurons have a leading role in the control of the egg-laying rhythm in Drosophila females.

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Genetic atlas of hygro-and thermosensory cells in the vinegar fly Drosophila melanogaster

Corthals,

Andersson,

Churcher

et al. 2023

Sci Rep

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The ability of animals to perceive and respond to sensory information is essential for their survival in diverse environments. While much progress has been made in understanding various sensory modalities, the sense of hygrosensation, which involves the detection and response to humidity, remains poorly understood. In this study, we focused on the hygrosensory, and closely related thermosensory, systems in the vinegar fly Drosophila melanogaster to unravel the molecular profile of the cells of these senses. Using a transcriptomic analysis of over 37,000 nuclei, we identified twelve distinct clusters of cells corresponding to temperature-sensing arista neurons, humidity-sensing sacculus neurons, and support cells relating to these neurons. By examining the expression of known and novel marker genes, we validated the identity of these clusters and characterized their gene expression profiles. We found that each cell type could be characterized by a unique expression profile of ion channels, GPCR signaling molecules, synaptic vesicle cycle proteins, and cell adhesion molecules. Our findings provide valuable insights into the molecular basis of hygro- and thermosensation. Understanding the mechanisms underlying hygro- and thermosensation may shed light on the broader understanding of sensory systems and their adaptation to different environmental conditions in animals.

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Mating disrupts morning anticipation in Drosophila melanogaster females

Cited by 7 publications

References 54 publications

Circadian control of a sex-specific behaviour in Drosophila

Circadian control of a sex-specific behaviour in Drosophila

Circadian control of a sex-specific behaviour in Drosophila

Genetic atlas of hygro-and thermosensory cells in the vinegar fly Drosophila melanogaster

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