The study aimed to determine relationships among mobile learning in English speaking, selfefficacy and learning autonomy of Chinese Junior High School students and propose a program to enhance their speaking proficiency level through mobile-assisted language learning. On the basis of the findings, it was verified that the mobile-assisted English speaking learning can improve junior middle school students' self-efficacy and autonomy in their English speaking proficiency. The research used the descriptive quantitative research design that made full use of the convenience of online survey and selected 426 respondents from four different junior high schools, with a relatively even proportion of eight classes. Based on the 426 valid questionnaires, the results showed that there is a highly significant relationship among mobile learning in English speaking, self-efficacy and learning autonomy of Chinese junior high students. The study revealed that students agree on mobile learning in English speaking, mobile learning self-efficacy, and learning autonomy. The mobile-assisted language learning can not only motivate students to study independently, but also develop strong will to improve their speaking proficiency level. Nevertheless, there were no significant differences in responses when grouped according to profile variables; moreover, the correlations of mobile learning, self-efficacy, and autonomy in learning were highly significant. Based on the findings, the English enhancement program was proposed, aiming to combine the usage of attitudes, resources, self-management, selfmonitoring and implementation in integrated English speaking learning involving mobile-assisted language learning, self-efficacy, and autonomy in learning. It is hoped that the study may be beneficial to enhance junior high students' mobile learning in English speaking, promote their self-efficacy and improve the effectiveness of autonomy in learning English speaking for Chinese Junior High School students speaking proficiency level.