Mathematical validation of a biological model for unlinking replication catenanes by recombination

Jayaram, Makkuni

doi:10.1073/pnas.1321214111

Cited by 2 publications

(1 citation statement)

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“…13). This intuitive model, based on product distributions observed in in vitro reactions with plasmid substrates, has been validated mathematically by a combination of tangle analysis and knot theory under the assumption that each recombination event reduces the topological complexity of the substrate (106,107).…”

Section: Topological and Chiral Features Of Tyrosine Recombinationmentioning

confidence: 99%

An Overview of Tyrosine Site-specific Recombination: From an Flp Perspective

Jayaram

Kachroo

et al. 2015

Microbiol Spectr

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Tyrosine site-specific recombinases (YRs) are widely distributed among prokaryotes and their viruses, and were thought to be confined to the budding yeast lineage among eukaryotes. However, YR-harboring retrotransposons (the DIRS and PAT families) and DNA transposons (Cryptons) have been identified in a variety of eukaryotes. The YRs utilize a common chemical mechanism, analogous to that of type IB topoisomerases, to bring about a plethora of genetic rearrangements with important physiological consequences in their respective biological contexts. A subset of the tyrosine recombinases has provided model systems for analyzing the chemical mechanisms and conformational features of the recombination reaction using chemical, biochemical, topological, structural, and single molecule-biophysical approaches. YRs with simple reaction requirements have been utilized to bring about programmed DNA rearrangements for addressing fundamental questions in developmental biology. They have also been employed to trace the topological features of DNA within high-order DNA interactions established by protein machines. The directed evolution of altered specificity YRs, combined with their spatially and temporally regulated expression, heralds their emergence as vital tools in genome engineering projects with wide-ranging biotechnological and medical applications.

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Section: Topological and Chiral Features Of Tyrosine Recombinationmentioning

confidence: 99%