Mathematical Modelling of Bioethanol Production from Raw Sugar Beet Cossettes in a Horizontal Rotating Tubular Bioreactor

Pavlečić, Mladen; Novák, Michal; Trontel, Antonija; Marđetko, Nenad; Grubišić, Marina; Ljubas, Blanka Didak; Tominac, Vlatka Petravić; Rakovac, Rozelindra Čož; Šantek, Božidar

doi:10.3390/fermentation8010013

Cited by 2 publications

(4 citation statements)

References 34 publications

(42 reference statements)

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“…The composition of carbohydrates, alcohols and organic acids in the SBC fermentation samples and weak acid ESBC hydrolysates were determined by ultra pressure liquid chromatography coupled with a refractive index detector (UPLC RID) analysis according to Marđetko et al ( 11 ). Bioprocess efficiency parameters were calculated according to Pavlečić et al ( 42 ):…”

Section: Methodsmentioning

confidence: 99%

“…Theoretical substrate conversion into ethanol ( Y E/ST ) was 0.538 g/g because the main sugar in the raw SBC is sucrose ( 42 ). In this study, all experiments were repeated at least once and the standard deviation of all measurements was in the range of experimental error (below 4.7 %).…”

Section: Methodsmentioning

confidence: 99%

“…where Y S is total consumption of substrates (g/L), C S0 is initial concentration of substrate (g/L), C S is final concentration of substrate (g/L), Y E is total ethanol yield (g/L), C E is final concentration of ethanol (g/L), C E0 is initial concentration of ethanol (g/L),Y E/S is conversion coefficient of substrate to ethanol (g/g), E is bioprocess efficiency (%), Y E/ST is theoretical conversion coefficient of substrate into ethanol (g/g; 0.538 g/g) Pr is bioprocess productivity (g/(L•h) and t is time (h). Theoretical substrate conversion into ethanol (Y E/ST ) was 0.538 g/g because the main sugar in the raw SBC is sucrose (42). In this study, all experiments were repeated at least once and the standard deviation of all measurements was in the range of experimental error (below 4.7 %).…”

Section: Analytical Methods and Calculation Of Bioprocess Efficiency ...mentioning

confidence: 99%

“…The maximum consumption rate of glucose was 0.496 g/(g . h), while for arabinose it was only 0.086 g/(g•h), calculated according to Pavlečić et al (42). The yeast entered the exponential phase of growth in the 4th hour of cultivation and the stationary phase after the 10th hour.…”

Section: Cultivation Of Yeasts Spathaspora Passalidarum Cbs 10155 And...mentioning

confidence: 99%

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Development of an Integrated Bioprocess System for Bioethanol and Arabitol Production from Sugar Beet Cossettes

Novak,

Marđetko,

Trontel

et al. 2024

Food Technol. Biotechnol. (Online)

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Research background. An innovative integrated bioprocess system for bioethanol production from raw sugar beet cossettes (SBC) and arabitol from remaining exhausted sugar beet cossettes (ESBC) was studied. This integrated three-stage bioprocess system is an example of the biorefinery concept to maximise the use of raw SBC for the production of high value-added products such as sugar alcohols and bioethanol. Experimental approach. The first stage of the integrated bioprocess system was simultaneous sugar extraction from SBC and its alcoholic fermentation to produce bioethanol in an integrated bioreactor system (vertical column bioreactor and stirred tank bioreactor) containing a high-density suspension of yeast Saccharomyces cerevisiae (30 g/L). The second stage was the pretreatment of ESBC with dilute sulfuric acid to release fermentable sugars. The resulting liquid hydrolysate of ESBC was used in the third stage as a nutrient medium for arabitol production by non-Saccharomyces yeasts (Spathaspora passalidarum CBS 10155 and Spathaspora arborariae CBS 11463). Results and conclusions. The obtained results show that the efficiency of bioethanol production increased with increasing temperature and prolonged residence time in the integrated bioreactor system. The maximum bioethanol production efficiency (87.22 %) was observed at a time of 60 min and a temperature of 36 °C. Further increase in residence time (above 60 min) did not result in the significant increase of bioethanol production efficiency. Weak acid hydrolysis was used for ESBC pretreatment and the highest sugar yield was reached at 200 °C and residence time of 1 min. The inhibitors of the weak acid pretreatment were produced below bioprocess inhibition threshold. The use of the obtained liqiud phase of ESBC hydrolysate for the production of arabitol in the stirred tank bioreactor under constant aeration clearly showed that S. passalidarum CBS 10155 with 8.48 g/L of arabitol (YP/S=0.603 g/g and bioprocess productivity of 0.176 g/(L·h)) is a better arabitol producer than Spathaspora arborariae CBS 10155. Novelty and scientific contribution. An innovative integrated bioprocess system for the production of bioethanol and arabitol was developed based on the biorefinery concept. This three-stage bioprocess system shows great potential for maximum use of SBC as a feedstock for bioethanol and arabitol production and it could be an example of a sustainable ‘zero waste’ production system.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Analytical Methods and Calculation Of Bioprocess Efficiency ...mentioning

confidence: 99%

Section: Cultivation Of Yeasts Spathaspora Passalidarum Cbs 10155 And...mentioning

confidence: 99%

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Development of an Integrated Bioprocess System for Bioethanol and Arabitol Production from Sugar Beet Cossettes

Novak,

Marđetko,

Trontel

et al. 2024

Food Technol. Biotechnol. (Online)

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Development of a Two-Stage Bioprocess for the Production of Bioethanol from the Acid Hydrolysate of Brewer’s Spent Grain

Vičević,

Božinović,

Zekić

et al. 2024

Energies

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Bioethanol, an alcohol produced by microbial fermentation, is traditionally produced from sugar-rich plants such as sugar cane, sugar beet and maize. However, there is growing interest in the use of lignocellulose, an abundant and inexpensive renewable energy source, as a potential substitute for the production of biofuels and biochemicals. Yeast Saccharomyces cerevisiae, which is commonly used for ethanol fermentation, cannot cope with lignocellulose due to a lack of lignocellulolytic enzymes and the inefficient functioning of the pentose phosphate pathway. The aim of this research was to isolate yeasts that can efficiently produce bioethanol and valuable byproducts from both glucose and xylose in a two-stage fermentation process using brewer’s spent grains. This approach should maximize sugar utilization and improve the economic viability of bioethanol production while contributing to waste valorization and sustainability. Kluyveromyces marxianus and Candida krusei were identified and tested with different initial concentrations of glucose and xylose. The results showed that both yeasts produced bioethanol from glucose but were inefficient with xylose, yielding valuable compounds, such as 2,3-butanediol and glycerol instead. A two-stage fermentation was then carried out with weak acidic hydrolysate from brewer’s spent grain. In the first stage, glucose was fermented by S. cerevisiae to produce bioethanol; in the second stage, xylose was fermented by K. marxianus and C. krusei to obtain other valuable products.

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Mathematical Modelling of Bioethanol Production from Raw Sugar Beet Cossettes in a Horizontal Rotating Tubular Bioreactor

Cited by 2 publications

References 34 publications

Development of an Integrated Bioprocess System for Bioethanol and Arabitol Production from Sugar Beet Cossettes

Development of an Integrated Bioprocess System for Bioethanol and Arabitol Production from Sugar Beet Cossettes

Development of a Two-Stage Bioprocess for the Production of Bioethanol from the Acid Hydrolysate of Brewer’s Spent Grain

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