Mathematical Modelling of an Enzyme-Based Biosensor

AS, Olufemi

doi:10.15406/ijbsbe.2017.03.00062

Cited by 3 publications

(1 citation statement)

References 7 publications

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“…FV -1 is next generation matrix, where F and V both are Jacobian of S and Λ evaluated for Enzyme, Substrate, and Complex at an equilibrium point. 19,20,21 Where, X=(s,c) t , Σ(X) denotes the entrance to the compartment and Λ(X) denotes transferring to another compartment or leaving the system. Then the Jacobian at the equilibrium point is given by As a result, Theorem: If the determinant of a matrix is non-zero then its inverse does exist.…”

Section: The Stability Of This Systemmentioning

confidence: 99%

Study the Dynamic Behavior of the Enzyme-Substrate Reaction using Mathematical Modeling

Patel*,

Kumawat

2023

Biosci., Biotech. Res. Asia

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Physiological reaction plays a vital role in the human body. These reactions are analysed through Enzyme kinetics using a Mathematical model which helps to predict how enzymes behave in living organisms. However, many factors affect the working mechanism of biocatalysts (Enzymes). Chemical denaturant creates high disruption to the structure of enzyme with time. The determination of enzyme activities with time delivers information on enzyme parameters. Here the analysis aims to mathematical study for the development of Enzyme - substrates reaction for product formation based on time. So we formulate the model as a system of nonlinear differential equations which predicts the behaviour of product formation based on Enzyme- Substrate reaction parameters. Compute the threshold value for studying the enzyme effectiveness, complexity, and other parameters for the substrate product. Study the stability analysis for the ideal product formation and hence derive asymptotically stable solutions for the Enzyme- Substrate model with numerical simulation.

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Section: The Stability Of This Systemmentioning

confidence: 99%

Study the Dynamic Behavior of the Enzyme-Substrate Reaction using Mathematical Modeling

Patel*,

Kumawat

2023

Biosci., Biotech. Res. Asia

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Kinetic model parameter estimation using genetic algorithms of the oxidation of phenol in water catalyzed by the laccase enzyme for the design of a biosensor

Navajas

Roa-Prada

Chalela-Alvarez

2021

Bioremediation Journal

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Detection of multi-residue insecticides in bitter gourd using Glutathione-S-Transferase enzyme based different analytical techniques

Mawtham,

Bhuvaneswari,

Thirumalairajan

et al. 2024

Preprint

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The food safety issues related to the pesticide residues in agricultural produce have increased the demand for instruments that can rapidly, sensitively, and selectively detect pesticide residues in food commodities. This idea served as the impetus for the development of protocols for the detection and quantification of various insecticide residues using GST-based biosensor probes. We demonstrated the effectiveness of GST-based biosensor and compared with analytical methods such as GC-MS/LC-MS for application in detection of multi-residue insecticides viz., synthetic pyrethroid and neonicotinoid in bitter gourd samples. Spectrophotometric analysis was confirmed from Michaelis-Menten constant (Km) and maximum velocity (Vmax) in the range 0.00178–0.00767 M and 0.01519–0.05249 mM/s for six different insecticides. Further, GST-based biosensor for the detection of various insecticides showed good linear curve (R2 > 0.98) in the range from 0.01 to 0.5 mg kg− 1 with LOD and LOQ calculated in 0.01 and 0.05 mg kg− 1. Method validation parameters namely linearity, recovery inhibition were calculated 1.83–65.10%, accuracy (71–100%) and precision (RSD < 6.0%), respectively. Among the 20 farmgate and market samples subjected to different techniques, imidacloprid residues were detected in four bitter gourd samples ranging from 0.042 to 0.099 mg kg− 1 in LC-MS and 0.050–0.10 mg kg− 1 for three samples in GST-based biosensor techniques.

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Mathematical Modelling of an Enzyme-Based Biosensor

Cited by 3 publications

References 7 publications

Study the Dynamic Behavior of the Enzyme-Substrate Reaction using Mathematical Modeling

Study the Dynamic Behavior of the Enzyme-Substrate Reaction using Mathematical Modeling

Kinetic model parameter estimation using genetic algorithms of the oxidation of phenol in water catalyzed by the laccase enzyme for the design of a biosensor

Detection of multi-residue insecticides in bitter gourd using Glutathione-S-Transferase enzyme based different analytical techniques

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