Mathematical modeling of glycerol fermentation by Klebsiella pneumoniae: Concerning enzyme-catalytic reductive pathway and transport of glycerol and 1,3-propanediol across cell membrane

Sun, Yu; Qi, Wenchuang; Teng, Hu; Xiu, Zhi Long; Zeng, An‐Ping

doi:10.1016/j.bej.2007.06.002

Cited by 108 publications

(73 citation statements)

References 27 publications

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“…Glycerol metabolism by K. pneumoniae under anaerobic condition has been stoichiometrically analyzed in previous studies [25,30,32]. The physiological mechanism of sequential products synthesis has been studied with 0.65 vvm air sparging [31] and the metabolic fluxes of glycerol in K. pneumoniae have been analyzed in batch and fed-batch fermentations under anaerobic and microaerobic conditions [7,19].…”

Section: Abbreviations C S0mentioning

confidence: 99%

Effect of aeration strategy on the metabolic flux of Klebsiella pneumoniae producing 1,3-propanediol in continuous cultures at different glycerol concentrations

Wang

Teng

Xiu

2010

J Ind Microbiol Biotechnol

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The microbial production of 1,3-propaneidol (1,3-PD) by Klebsiella pneumoniae in continuous fermentation was investigated under low, medium and high glycerol concentrations in the absence and presence of oxygen. The production of 1,3-PD increased with increasing glycerol concentrations, reaching a maximum (266 mmol l⁻¹) under high glycerol concentration (760 mmol l⁻¹) with air sparging at 0.04 vvm. The yield of 1,3-PD, however, decreased gradually with increasing glycerol concentrations, with the highest yield (0.52 mol mol⁻¹) obtained for low glycerol concentration (270 mmol l⁻¹) under anaerobic condition. Enzyme activity assays showed that the specific activity of glycerol dehydratase was highest (0.04 U mg⁻¹) for culture sparged with 0.04 vvm air under high glycerol concentration. The specific activities of glycerol dehydrogenase and 1,3-propanediol oxidoreductase were also improved for all glycerol concentrations and in the presence of oxygen, implying that the dha operon was not repressed under microaerobic conditions. Analysis of metabolic fluxes showed that more carbon flux was shifted to the oxidative pathway with increasing glycerol concentrations, resulting in a reduced flux to 1,3-PD formation. However, the increases in carbon fluxes were not evenly distributed among the oxidative branches of the pathway. Furthermore, ethanol and acetic acid levels were slightly increased whereas 2,3-butanediol and lactic levels were greatly enhanced.

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Section: Abbreviations C S0mentioning

confidence: 99%

Effect of aeration strategy on the metabolic flux of Klebsiella pneumoniae producing 1,3-propanediol in continuous cultures at different glycerol concentrations

Wang

Teng

Xiu

2010

J Ind Microbiol Biotechnol

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“…In this paper, we focus on the batch culture process with glycerol as the substrate. Previous research indicates that this batch culture process is highly promising for producing commercially viable 1,3-PD of high concentration [2][3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Robust Optimal Control of a Microbial Batch Culture Process

Cheng

Wang

Loxton

et al. 2014

J Optim Theory Appl

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This paper considers the microbial batch culture process for producing 1,3-propanediol (1,3-PD) via glycerol fermentation. Our goal was to design an optimal control scheme for this process, with the aim of balancing two (perhaps competing) objectives: (i) the process should yield a sufficiently high concentration of 1,3-PD at the terminal time and (ii) the process should be robust with respect to changes in various uncertain system parameters. Accordingly, we pose an optimal control problem, in which both process yield and process sensitivity are considered in the objective function. The control variables in this problem are the terminal time of the batch culture process and the initial concentrations of biomass and glycerol in the batch reactor. By performing a time-scaling transformation and introducing an auxiliary dynamic system to calculate process sensitivity, we obtain an equivalent optimal control problem Communicated by Mimmo Iannelli. 123J Optim Theory Appl in standard form. We then develop a particle swarm optimization algorithm for solving this equivalent problem. Finally, we explore the trade-off between process efficiency and process robustness via numerical simulations.

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“…(Sun et al, 2008). A formação de biomassa no processo fermentativo depende de duas moléculas transportadoras de energia, o NAD + (dinucleotídeo de adenina nicotinamida) e o ATP (adenosina trifosfato) (Kaur et al, 2012).…”

Section: Introductionunclassified

Estudo Cinético Do Crescimento Do Clostridium Acetobutylicum Atcc 4259 Utilizando Glicerol P.A. Como Substrato

Lacerda¹,

Vinhas²,

Almeida³

2015

Anais Do XX Congresso Brasileiro De Engenharia Química

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RESUMO -A glicerina, subproduto da produção de biodiesel, vem sendo investigada como fonte de carbono em processos microbianos para a obtenção de bioprodutos com alto valor agregado. A avaliação do crescimento do microorganismo responsável por essa bioconversão é uma importante etapa desse processo. O presente trabalho consistiu em avaliar a cinética de crescimento do Clostridium acetobutylicum ATCC 4259 através de uma fermentação em batelada utilizando glicerol P.A. como fonte de carbono. A técnica de peso seco foi utilizada para o calculo da concentração do micro-organismo presente no meio de cultura após a fermentação. A cinética microbiana mostrou que, para as condições experimentais da pesquisa, a fase exponencial de crescimento ocorreu até o tempo de 14 horas, e uma produtividade celular (P) de 0,0405 g/L.h, uma velocidade máxima de crescimento (µ máx ) de 0,0403 h -1 e um tempo de geração (G) de 15,7 h foram alcançados.

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Mathematical modeling of glycerol fermentation by Klebsiella pneumoniae: Concerning enzyme-catalytic reductive pathway and transport of glycerol and 1,3-propanediol across cell membrane

Cited by 108 publications

References 27 publications

Effect of aeration strategy on the metabolic flux of Klebsiella pneumoniae producing 1,3-propanediol in continuous cultures at different glycerol concentrations

Effect of aeration strategy on the metabolic flux of Klebsiella pneumoniae producing 1,3-propanediol in continuous cultures at different glycerol concentrations

Robust Optimal Control of a Microbial Batch Culture Process

Estudo Cinético Do Crescimento Do Clostridium Acetobutylicum Atcc 4259 Utilizando Glicerol P.A. Como Substrato

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