Mathematical modeling of fed-batch fermentation of Schizochytrium sp. FJU-512 growth and DHA production using a shift control strategy

Zhang, Mingliang; Wu, Weibin; Guo, Xiaolei; Weichen, You; Qi, Feng; Jiang, Xianzhang; Huang, Jianzhong

doi:10.1007/s13205-018-1187-1

Cited by 12 publications

(5 citation statements)

References 29 publications

(36 reference statements)

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“…When the rotation speed was 130 rpm, the biomass of Aurantiochytrium.SW1 was all below 10 g/L and when the rotation speed was 270 rpm, the biomass of Aurantiochytrium SW1 was all at a higher level (11.26 -15.32 g/L). This was due to the fact when the oxygen in the medium was insufficient, it was difficult to sustain the growth of Aurantiochytrium SW1.This is consistent with the study of Zhang et al (2018), which also showed that oxygen is an important factor for the growth of Aurantiochytrium SW1. It was found that the addition of a moderate amount of glucose also contributed to the growth of Aurantiochytrium SW1 at lower rotational speeds.…”

Section: Optimization Of Mficroalgal Biomasssupporting

confidence: 86%

Producing Functional Fatty Acid DHA from Corn Steep Liquor by Aurantiochytrium SW1

Ren¹,

Qi²,

Liu³

et al. 2021

American Journal of Biochemistry and Biotechnology

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Thraustochytrium has a relatively high lipid content in the cell and its lipid content can account up to 70% of the dry cell weight. It is an important raw material for the third-generation biodiesel production and the production of functional fatty acid Docosahexaenoic Acid (DHA). However, the high cost of traditional fermentation medium has hindered the industrial production of lipid. Therefore, searching for raw materials that can be used by thraustochytrid with a wide range of sources and low prices has become a research hotspot in the production of lipids by thraustochytrid. In this study, corn steep liquor was applied for lipid production in thraustochytrid Aurantiochytrium SW1, various factors of corn medium were optimized by using Response Surface Methodology (RSM), including glucose concentration, Rotation Speed, Kinetin concentration and dilution rate of corn steep liquor. The optimal conditions were finally obtained as 72.37 g/L glucose, 2.28 mg/L kinetin, 270 rpm of rotation speed and 15 times of corn pulp dilution. The biomass, lipid and DHA yields obtained under the optimized conditions were 13.5, 7.9 and 42% of total fatty acids, respectively. Comparison with the traditional medium, the biomass, lipid and DHA production were promoted by 14.8, 31.6 and 31.3% respectively. The optimized medium using cheap corn steep liquor showed a better production level, which proved the feasibility of culturing Aurantiochytrium SW1 with cheap raw materials.

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Section: Optimization Of Mficroalgal Biomasssupporting

confidence: 86%

Producing Functional Fatty Acid DHA from Corn Steep Liquor by Aurantiochytrium SW1

Ren¹,

Qi²,

Liu³

et al. 2021

American Journal of Biochemistry and Biotechnology

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“…A study conducted by Zhang et al . 38 reported that a higher agitation speed (between the range of 300–350 rpm) was required to achieved high cell densities but moderate agitation speed (200–250 rpm) was optimal for substantial lipid and DHA accumulation in Schizochytrium Sp. FJU-512.…”

Section: Resultsmentioning

confidence: 99%

Optimization of Culture Conditions for Enhanced Growth, Lipid and Docosahexaenoic Acid (DHA) Production of Aurantiochytrium SW1 by Response Surface Methodology

Nazir

Shuib

Kalil

et al. 2018

Sci Rep

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In this study, optimization of growth, lipid and DHA production of Aurantiochytrium SW1 was carried out using response surface methodology (RSM) in optimizing initial fructose concentration, agitation speed and monosodium glutamate (MSG) concentration. Central composite design was applied as the experimental design and analysis of variance (ANOVA) was used to analyze the data. ANOVA analysis revealed that the process which adequately represented by quadratic model was significant (p < 0.0001) for all the response. All the three factors were significant (p < 0.005) in influencing the biomass and lipid data while only two factors (agitation speed and MSG) gave significant effect on DHA production (p < 0.005). The estimated optimal conditions for enhanced growth, lipid and DHA production were 70 g/L fructose, 250 rpm agitation speed and 10 g/L MSG. Consequently, the quadratic model was validated by applying the estimated optimum conditions, which confirmed the model validity where 19.0 g/L biomass, 9.13 g/L lipid and 4.75 g/L of DHA were produced. The growth, lipid and DHA were 28, 36 and 35% respectively higher than that produced in the original medium prior to optimization.

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“…Generally, the product formation was categorized into three categories: growth‐associated product, non‐growth‐associated product, and mixed growth‐associated product 31. In this study, DHA is the product of fermentation which can be categorized in mixed growth associated since DHA production is related to the enhanced lipid and biomass accumulation 32.…”

Section: Resultsmentioning

confidence: 99%

Optimal Feeding Strategy in Fermentation of Docosahexaenoic Acid Production by Schizochytrium sp.

et al. 2022

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Fed-batch fermentation has the potential to increase the production of docosahexaenoic acid (DHA) in Schizochytrium sp. which can provide additional nutrients. The feeding strategy in fed-batch fermentation is one of the important aspects that could help in improving the DHA yield. An enhanced scatter search (eSS) method and the control vector parametrization (CVP) approach were applied to generate the optimal feeding trajectory numerically. Three objective functions were considered, namely, maximizing the DHA concentration, minimizing the final time, and maximizing the economic function. The shortest time and maximum DHA could be determined simultaneously from the optimal feeding trajectory in maximizing the economic function.

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Mathematical modeling of fed-batch fermentation of Schizochytrium sp. FJU-512 growth and DHA production using a shift control strategy

Cited by 12 publications

References 29 publications

Producing Functional Fatty Acid DHA from Corn Steep Liquor by Aurantiochytrium SW1

Producing Functional Fatty Acid DHA from Corn Steep Liquor by Aurantiochytrium SW1

Optimization of Culture Conditions for Enhanced Growth, Lipid and Docosahexaenoic Acid (DHA) Production of Aurantiochytrium SW1 by Response Surface Methodology

Optimal Feeding Strategy in Fermentation of Docosahexaenoic Acid Production by Schizochytrium sp.

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