A bomb calorimeter is an apparatus used for measuring the performance of coal in term of heat of combustion. Recent awareness has been created regarding uncertainty of measurement, due to mainly two reasons. Laboratory accreditation, which has steadily been on the rise, which requires a estimation of uncertainty of measurement particularly in the field of calibration. Second, increased maturity level of the quality system certification as the manufacturing companies looking at the reliability of measurement through correct calibration of inspection, measuring and test equipment. The quality of coal is decided by the various parameters such as ash content, air dried moisture, volatile matter, gross calorific value and sulphur content etc. The grade of coal is decided by the quality of coal which decides the performance of generation of electricity. The uncertainty of measurement in bomb calorimeter gets affected by various parameters. This paper describes the methodology, uncertainty calculations and effects of individual factors on it.