Mathematical analysis of frontal affinity chromatography in particle and membrane configurations

Tejeda-Mansir, Armando; Montesinos, Rosa M.; Guzmán, Roberto

doi:10.1016/s0165-022x(01)00196-8

Cited by 48 publications

(19 citation statements)

References 66 publications

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“…A standard solution of OTA (50 ppb) was employed in frontal chromatography to determine the binding capacity of each and every frit. Frontal chromatography is a well-established measurement technique that takes both surface area and sub-surface porosity into account, yielding one single data (59,60). The capacity (or the selectivity) provided by MIPPy/CNT can be properly attributed to the CNTs (or the molecular imprinting).…”

Section: High Binding Capacity Via Surface Area Enhancementmentioning

confidence: 99%

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Molecularly Imprinted Polymer Nanomaterials for Mycotoxin Extraction

Lai

2009

ACS Symposium Series

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A new electrochemical imprinting technique, in which pyrrole is used as a functional monomer, has great potential to glue nanomaterials together. The molecularly imprinted polypyrrole (MIPPy), using ochratoxin A (OTA) as the template molecule, is electrochemically deposited onto carbon nanotubes (CNTs) or quantum dots (QDs) to make a solid phase of nanoscale for specific extraction of the mycotoxin. This MIPPy nanomaterial is simply structured within the 0.5-μm pores of a stainless steel frit, or inside a syringe needle, to make a novel micro solid phase extraction (µSPE) device. With the nanostructure of CNTs or QDs, the total number of specific binding sites of MIPPy-on-a-frit or MIPPy-in-aneedle is significantly enhanced for binding of OTA at subppb levels even in the presence of abundant red wine matrix components. Molecularly Imprinted PolymersOver the last decade, molecularly imprinted polymers (MIPs) have gained more acceptance regarding their application as synthetic receptors for analytical techniques such as liquid chromatography, capillary electrochromatography, solid phase extraction, immunoassay, and chemical sensors (1). MIPs are solid materials that can be synthesized in the presence of a target compound (the template molecule) through polymerization. After removal of template, specific Downloaded by UCSF LIB CKM RSCS MGMT on November 18, 2014 |

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Section: High Binding Capacity Via Surface Area Enhancementmentioning

confidence: 99%

“…Leakage of OTA from the frit was monitored over storage periods ranging from hours to days. In good laboratory practice, a couple of pulsed elutions can readily clean out any OTA leakage prior to using the frit for MIPPy/CNT-μSPP-HPLC analysis of red wine samples (59).…”

Section: Stability Of Mippy/cnt-modified Stainless Steel Fritsmentioning

confidence: 99%

Molecularly Imprinted Polymer Nanomaterials for Mycotoxin Extraction

Lai

2009

ACS Symposium Series

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“…The lumped parameter model [24][25][26] has been successfully used to describe batch adsorption processes. This model takes an empirical approach to the adsorption process and assumes that all the rate-limiting steps can be represented by a kinetic rate constant.…”

Section: Introductionmentioning

confidence: 99%

“…[26] The development of mathematical models to describe these processes, and the use of these models in computer programs to predict system performance is an engineering approach that can help to successfully attain these bioprocess-engineering tasks.…”

Section: Introductionmentioning

confidence: 99%

Batch Equilibrium and Kinetic Studies of Plasmid pCI Adsorption onto Perfusion Particles

Pérez-Martínez

Montesinos-Cisneros

Guerrero-Germán

et al. 2014

Journal of Liquid Chromatography & Related Technologies

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The demand for efficient production methods of plasmid DNA (pDNA) has increased vastly in response to rapid advances in the use of pDNA for third-generation vaccines. The process development requires conduct basic studies in batch format to determine the characteristic parameters of the biomolecule-adsorbent system. In this work, the equilibrium and kinetic performance of the plasmid pCI adsorption onto ion-exchange perfusive particles POROS 50 HQ was studied. The isotherm obtained was appropriately described by the Langmuir model, with the parameters q m ¼ 29.15 mg PCI=mL of adsorbent and K d ¼ 0.0145 mg de pCI=mL. These values show a greater capacity and more specific adsorption of pCI onto the anion-exchange (AE) perfusive particles then onto AE adsorptive membranes previously studied. The kinetics of adsorption was described adequately by a lumped resistance model using a kinetic constant of k 1 ¼ 0.0067 mL=mg s.

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“…Besides numerous systems for laboratory scale applications, modular systems of membrane adsorbers for a technical scale in pharmaceutical downstream processing have been reported [7,11,12]. Numerous comprehensive reviews discuss the fundamentals and applications of membrane chromatography [13][14][15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Breakthrough performance of linear-DNA on ion-exchange membrane columns

Montesinos-Cisneros

Ortega

Guzmán

et al. 2006

Bioprocess Biosyst Eng

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Breakthrough performance of linear-DNA adsorption on ion-exchange membrane columns was theoretically and experimentally investigated using batch and fixed-bed systems. System dispersion curves showed the absence of flow non-idealities in the experimental arrangement. Breakthrough curves were not significantly affected by flow-rate or inlet solution concentration. In the theoretical analysis a model was integrated by the serial coupling of the membrane transport model and the system dispersion model. A transport model that considers finite kinetic rate and column dispersed flow was used in the study. A simplex optimization routine coupled to the solution of the partial differential model equations was employed to estimate the maximum adsorption capacity constant, the equilibrium desorption constant and the forward interaction rate-constant, which are the parameters of the membrane transport model. Through this approach a good prediction of the adsorption phenomena is obtained for inlet concentrations and flow rates greater than 0.2 mg/ml and 0.16 ml/min.

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Mathematical analysis of frontal affinity chromatography in particle and membrane configurations

Cited by 48 publications

References 66 publications

Molecularly Imprinted Polymer Nanomaterials for Mycotoxin Extraction

Molecularly Imprinted Polymer Nanomaterials for Mycotoxin Extraction

Batch Equilibrium and Kinetic Studies of Plasmid pCI Adsorption onto Perfusion Particles

Breakthrough performance of linear-DNA on ion-exchange membrane columns

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