a b s t r a c tWe study the cohomology groups with Z 2 -coefficients for compact flat Riemannian manifolds of diagonal type M Γ = Γ \ R n by explicit computation of the differentials in the Lyndon-Hochschild-Serre spectral sequence. We obtain expressions for H j (M Γ , Z 2 ), j = 1, 2 and give an effective criterion for the non-vanishing of the second Stiefel-Whitney class w 2 (M Γ ). We apply the results to exhibit isospectral pairs with special cohomological properties; for instance, we give isospectral 5-manifolds with different H 2 (M Γ , Z 2 ), and isospectral 4-manifolds M, M having the same Z 2 -cohomology where w 2 (M) = 0 and w 2 (M ) = 0. We compute the Z 2 -cohomology of all generalized Hantzsche-Wendt n-manifolds for n = 3, 4, 5 and we study H 2 and w 2 for a large n-dimensional family, K n , with explicit computation for a subfamily of examples due to Lee and Szczarba.