Maternal β-hydroxy-β-methylbutyrate (HMB) supplementation during pregnancy affects early folliculogenesis in the ovary of newborn piglets

Hułas-Stasiak, Monika; Jakubowicz-Gil, Joanna; Dobrowolski, Piotr; Tomaszewska, Ewa; Muszyński, Siemowit

doi:10.1016/j.theriogenology.2019.02.003

Cited by 7 publications

(6 citation statements)

References 60 publications

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“…The results obtained from the current study are in accordance with earlier studies and confirm the action of HMB and AKG as metabolism stimulants and energy donors [22,28,30,44]. The effects of HMB gestational supplementation were more abundant and stronger in males than in females, which is also confirmed by other studies which examined the effects of this leucine derivative on organs and body systems [23,43]. Although the HMB effects were less prominent or even inhibitory with regards to certain structural parameters assessed in the intestines of female offspring, the addition of AKG usually ameliorated these effects.…”

Section: Discussionsupporting

confidence: 92%

“…The current study focused on the structural adaptations of the gastrointestinal tract in offspring from primiparous and multiparous minks supplemented with HMB and/or AKG during gestation. Although previous studies carried out over the last two decades, based on the above-mentioned substances, have contributed to a broad knowledge base on the effects of HMB and AKG on the skeletal, gastrointestinal, nervous, integumentary, muscular and reproductive systems, only a few have studied the interactions between HMB and AKG and their long-term effects [3,20,21,23,28,30,31,43,44]. However, in these studies, less attention was placed on the gastrointestinal tract and in particular on the prenatal effects of nutritional manipulations on its postnatal development and structure.…”

Section: Discussionmentioning

confidence: 99%

“…From this perspective feed additives like β-hydroxy-βmethylbutyrate (HMB) and alpha-ketoglutaric acid (AKG) have potential health benefits. Even though their role in prenatal programming has been assessed, not only in livestock, there are still many unknowns [12,[17][18][19][20][21][22][23]. Very limited knowledge about the effects of these amino acid precursors in fur animals exists in literature and there is no information about their effects on the digestive tract of minks.…”

Section: Introductionmentioning

confidence: 99%

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The Effects of Prenatal Supplementation with β-Hydroxy-β-Methylbutyrate and/or Alpha-Ketoglutaric Acid on the Development and Maturation of Mink Intestines Are Dependent on the Number of Pregnancies and the Sex of the Offspring

Dobrowolski

Muszyński

Donaldson

et al. 2021

Animals

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Prenatal and postnatal supplementation with β-hydroxy-β-methylbutyrate (HMB) and alpha-ketoglutaric acid (AKG) affects the development and maturation of offspring. Both substances have the potential to stimulate cell metabolism via different routes. However, parity affects development and may alter the effects of dietary supplementation. This study aimed to evaluate the effect of gestational supplementation with HMB and/or AKG to primiparous and multiparous minks on the structure and maturation of the offspring’s small intestine. Primiparous and multiparous American minks (Neovison vison), of the standard dark brown type, were supplemented daily with HMB (0.02 g/kg b.w.) and/or AKG (0.4 g/kg b.w.) during gestation (n = 7 for each treatment). Supplementation stopped when the minks gave birth. Intestine samples were collected from 8-month-old male and female offspring during autopsy and histology and histomorphometry analysis was conducted (LAEC approval no 64/2015). Gestational supplementation had a long-term effect, improving the structure of the offspring’s intestine toward facilitating absorption and passage of intestinal contents. AKG supplementation affected intestinal absorption (enterocytes, villi and absorptive surface), and HMB affected intestinal peristalsis and secretion (crypts and Goblet cells). These effects were strongly dependent on parity and offspring gender. Present findings have important nutritional implications and should be considered in feeding practices and supplementation plans in animal reproduction.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Effects of Prenatal Supplementation with β-Hydroxy-β-Methylbutyrate and/or Alpha-Ketoglutaric Acid on the Development and Maturation of Mink Intestines Are Dependent on the Number of Pregnancies and the Sex of the Offspring

Dobrowolski

Muszyński

Donaldson

et al. 2021

Animals

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“…; Lonza, Basel, Switzerland, purity ≥99%) was supplemented in one morning pellet between the 13th and 26th day of pregnancy to females from the HMB groups. The dose of HMB used was based on the literature data and previous studies from our own laboratory [ 19 , 34 , 35 , 36 , 37 , 38 , 39 , 40 ]. Females from all experimental groups were euthanized by CO 2 inhalation.…”

Section: Methodsmentioning

confidence: 99%

Femoral µCT Analysis, Mechanical Testing and Immunolocalization of Bone Proteins in β-Hydroxy β-Methylbutyrate (HMB) Supplemented Spiny Mouse in a Model of Pregnancy and Lactation-Associated Osteoporosis

Tomaszewska

Muszyński

Donaldson

et al. 2021

JCM

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A metabolite of leucine, ß-hydroxy-ß-methylbutyrate (HMB), used as a dietary supplement effects muscle tissue gain and bone tissue quality. Since there are no studies on the effects of HMB during pregnancy yet, the aim of the current study was to determine the effects of HMB supplementation during pregnancy on osteoporotic bone quality postpartum and post-lactation using spiny mice (Acomys cahirinus) as the animal models. The six-month-old dams were divided into four groups: pregnant and lactating controls, and pregnant and lactating HMB-treated (during the second trimester of pregnancy) females. The intensity of the immunoreaction of osteocalcin (OC), osteoprotegerin (OPG), bone morphogenetic protein 2 (BMP-2), tissue inhibitor of metalloproteinases 2 (TIMP-2), matrix metalloproteinase 8 and 13 (MMP-8 and MMP-13) and proteins involved in bone turnover, was measured in femoral trabecular and compact bone, as well as in the hyaline and epiphyseal cartilage of the femora. The analysis of the trabecular bone microarchitecture showed that the administration of HMB to pregnant females, by influencing the proteins responsible for bone cell activity and collagen remodeling, can provide protection from bone loss. Based on the results of the current study it can be assumed that HMB administration to pregnant females has a more positive impact on trabecular than compact bone.

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“…For supplementation was used HMB in the form of calcium salts, while pregnant sows from control groups were supplementation with placebo -CaCO 3 at the same dose. This HMB dose was selected based on previous studies that used the same dose of hydroxy-β-methylbutyrate and had a significant effect on offspring development [16,17,27]. In this study we decided to supplement sows between 70 to 90 days of pregnancy, because this is critical moment to prenatal development in piglets [28].…”

Section: Experimental Modelmentioning

confidence: 99%

The effect of maternal HMB supplementation on bone mechanical and geometrical properties, as well as histomorphometry and immunolocalization of VEGF, TIMP2, MMP13, BMP2 in the bone and cartilage tissue of the humerus of their newborn piglets

Tomczyk-Warunek

Blicharski

Jarecki

et al. 2020

Preprint

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The presented experiment focuses on assessing the impact of HMB ( hydroxy-β-methobutyrate ) supplementation of mothers during pregnancy on the development of the skeletal system of their offspring. For this purpose, an experiment was carried out on 12 clinically healthy sows of the Great White Poland breed, which were divided randomly into two groups the control and the HMB group. All animals were kept under standard conditions and received the same feed for pregnant females. In contrast, females from the HMB group between 70 and 90 days were supplemented with 3-hydroxy-3-methylbutyle in the amount of 0.2g/kg b.w/day. Immediately after birth, the piglets were also divided into groups based on: sex, and presence or lack HMB supplementation, and subsequently were euthanized and humerus bones from all piglets were collected. Mother’s HMB supplementation during pregnancy affected the multiple index of their offspring. The higher humerus mass and length was observed with the greater effect in males. Maternal supplementation also influenced on the geometrical and mechanical properties of the humerus as in the case of mass, this effect was higher in males. Also, the collagen structure of the compacted and trabecular bone changed under the HMB addition. Maternal supplementation also affected the expression of selected proteins in growth cartilage and trabecular bone. The obtained results show that the administration to the mother during pregnancy by the HMB significantly affects the development of the humerus in many ways. The obtained results also confirm the utility of such experiments in understanding of the importance of the pregnancy diet as an develop and adaptable factor of offspring organisms and are the base for further research in that area as well as in the protein markers expression area.

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Maternal β-hydroxy-β-methylbutyrate (HMB) supplementation during pregnancy affects early folliculogenesis in the ovary of newborn piglets

Cited by 7 publications

References 60 publications

The Effects of Prenatal Supplementation with β-Hydroxy-β-Methylbutyrate and/or Alpha-Ketoglutaric Acid on the Development and Maturation of Mink Intestines Are Dependent on the Number of Pregnancies and the Sex of the Offspring

The Effects of Prenatal Supplementation with β-Hydroxy-β-Methylbutyrate and/or Alpha-Ketoglutaric Acid on the Development and Maturation of Mink Intestines Are Dependent on the Number of Pregnancies and the Sex of the Offspring

Femoral µCT Analysis, Mechanical Testing and Immunolocalization of Bone Proteins in β-Hydroxy β-Methylbutyrate (HMB) Supplemented Spiny Mouse in a Model of Pregnancy and Lactation-Associated Osteoporosis

The effect of maternal HMB supplementation on bone mechanical and geometrical properties, as well as histomorphometry and immunolocalization of VEGF, TIMP2, MMP13, BMP2 in the bone and cartilage tissue of the humerus of their newborn piglets

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