Maternal transfer and transcriptional onset of immune genes during ontogenesis in Atlantic cod

Seppola, Marit; Johnsen, Hanne; Mennen, Saskia; Myrnes, Bjørnar; Tveiten, Helge

doi:10.1016/j.dci.2009.06.013

Cited by 85 publications

(58 citation statements)

References 53 publications

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“…So far, the LGP2 gene has been functionally characterized only in mammalian species, although functionally uncharacterized sequences have been described in Atlantic cod (EU371924, a partial cDNA) (28), grass carp (27), and Atlantic salmon (BT045378). To elucidate the evolutionary origins and primordial functions of LGP2 as one of the receptors that recognize PAMPs, our studies focused on the discovery of RLR family orthologs in teleosts.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the CARDs of RIG-I inhibited virus replication in Atlantic salmon (25). Other RLRs, such as MDA5 and LGP2, have not been been characterized functionally, although their cDNAs were cloned from grass carp (Ctenopharyngodon idella) and Atlantic cod (Gaudus morhua) (26)(27)(28). Furthermore, the LGP2 and Stat3/5 genes were syntenic in zebrafish (Danio rerio), stickleback (Gasterosteus aculeatus), medaka (Oryzias latipes), and fugu (29) as well as in mouse chromosome 11 (5).…”

Section: Rlrs That Bind To Viral Rnas Contribute To the Antiviral Actmentioning

confidence: 99%

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Evolutional Conservation of Molecular Structure and Antiviral Function of a Viral RNA Receptor, LGP2, in Japanese Flounder, Paralichthys olivaceus

Ohtani

Hikima

Kondo

et al. 2010

The Journal of Immunology

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LGP2 is an important intracellular receptor that recognizes viral RNAs in innate immunity. To understand the mechanism of viral RNA recognition, we cloned an LGP2 cDNA and gene in Japanese flounder (Paralichthys olivaceus). Viral hemorrhagic septicemia virus-induced expressions of LGP2 mRNA were evaluated in vivo and in vitro by quantitative real-time PCR (Q-PCR) using primers based on the clone sequences. The expression of LGP2 mRNA in the kidney dramatically increased at 3 d postinfection. The expression of LGP2 mRNA also increased in the head kidney leukocytes stimulated with artificial dsRNA (polyinosin-polycytidylic acid) in vitro. To evaluate the antiviral activity of the flounder LGP2, three expression constructs containing pcDNA4-LGP2 (full-length), pcDNA4-LGP2ΔRD (regulatory domain deleted), and pcDNA4-Empty (as a negative control) were transfected into the hirame (flounder) natural embryo (hirame natural embryo) cell line. Forty-eight hours after transfection, the transfected cells were infected with ssRNA viruses, viral hemorrhagic septicemia virus, or hirame rhabdovirus. The cytopathic effects of the viruses were delayed by the overexpression of Japanese flounder LGP2. The Q-PCR demonstrated that mRNA expression levels of type I IFN and IFN-inducible genes (Mx and ISG15) in the hirame natural embryo cells overexpressing LGP2 were increased by polyinosin-polycytidylic acid and viral infections. These results suggest that Japanese flounder LGP2 plays an important role in the recognition of both viral ssRNA and dsRNA to induce the antiviral activity by the production of IFN-stimulated proteins.

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Section: Discussionmentioning

confidence: 99%

Section: Rlrs That Bind To Viral Rnas Contribute To the Antiviral Actmentioning

confidence: 99%

Evolutional Conservation of Molecular Structure and Antiviral Function of a Viral RNA Receptor, LGP2, in Japanese Flounder, Paralichthys olivaceus

Ohtani

Hikima

Kondo

et al. 2010

The Journal of Immunology

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“…Both innate and adaptive types of immune factors are transferred from mother to offspring in fishes. Immunoglobulin, as the most important maternal immune factor in vertebrates (Picchietti et al, 2006), and other innate immune factors such as the complement components (Huttenhuis et al, 2006), lectins (Hasan et al, 2009), lysozymes (Magnadóttir et al, 2005), and antimicrobial peptides (Seppola et al, 2009) have been found to be transferred maternally by protein or mRNA in several fish species.…”

Section: Introductionmentioning

confidence: 99%

Effects of dietary β-glucan on maternal immunity and fry quality of rainbow trout (Oncorhynchus mykiss)

et al. 2015

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“…The hepcidin gene is widely expressed in various fish, suggesting that this antimicrobial peptide is a very important component in the innate immune system. During metamorphosis of the Atlantic cod, hepcidin showed a high level of transcriptional up-regulation at 1295 hpf (Seppola et al, 2009). The expression in other fish of this AMP is up regulated in several tissues and organs in response to a bacterial challenge (Shike et al, 2002;Park et al, 2005;Kim et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Many fish larvae have poorly developed immune response and are exposed to pathogens before their lymphoid organs have matured and adaptive immunity has developed (Seppola et al, 2009), even though these larvae may be protected by maternally transferred components, like lectins and antibodies (Bly et al, 1986;Avtalion & Mor, 1992;Seppola et al, 2009). The halibut has been characterized by several defense gene components like immunoglobulin light chain, MHC class I and II, antimicrobial peptide, complement system, CC chemokine and B-cell factor (Park et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Characterization of culturable bacterial flora in yolk sac larvae of Atlantic halibut (Hippoglossus hippoglossus L.) with

Urtubia¹,

Gallardo²,

Lavín³

et al. 2014

lajar

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ABSTRACT.One of the main problems facing Atlantic halibut hatcheries is the high mortality in the early stages of larval development. Several factors could be involved, for example: water quality, diseases or abnormalities, such as deformities occurring in the yolk sac larvae prior to exogenous feeding. The aim of this study was to identify differences in bacterial flora associated with yolk sac larvae with oral deformity. We also aimed to establish whether there is any relationship between bacterial strains and the "gaping jaws" syndrome. During our study, 74 bacterial isolates were obtained using three different nutrient media: Marine Agar, R2A and TCBS. Some of these bacteria were characterized using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and 16S rRNA sequencing. The immune response in larvae exhibiting the "gaping jaws" condition was measured by real time PCR. Our results showed significant differences in bacterial flora between normal and gaping larvae. The gaping yolk sac larvae were predominantly colonized by members of the families Vibrionaceae and Flavobacteriaceae. Bacteria belonging to the Bacillus and Pseudoalteromonas genera were also present but less frequent. It was not possible to associate a type or group of bacteria directly related to "gaping". Strikingly, larvae with gaping jaws had an increase in the expression of two immune related genes, like hepcidin and chemokine (MIP-1ß). These results indicate activation of the immune response in larvae with "gaping jaws" syndrome and this response could be related to bacteria isolated from gaping condition. Keywords: Atlantic halibut, Hippoglossus hippoglossus, bacterial flora, "gaping jaws", immune genes.Caracterización de la flora cultivable y la respuesta inmune en larvas con saco vitelino del halibut del Atlántico (Hippoglossus hippoglossus L.) con el síndrome de "gaping jaws" RESUMEN. Uno de los mayores problemas que enfrentan los criaderos de halibut del Atlántico es la alta mortalidad en estados tempranos del desarrollo larval. Distintos factores pueden estar involucrados, e.g., calidad del agua, enfermedades o anormalidades tales como deformidades que ocurren en las larvas en estado de saco vitelino antes de la alimentación exógena. El objetivo de este estudio fue identificar diferencias en la flora bacteriana asociada a larvas con saco vitelino con deformidad oral. Además se buscó establecer si existió relación entre alguna cepa bacteriana y el síndrome de "gaping jaws". Durante el estudio, 74 cepas bacterianas fueron aisladas usando diferentes medios de cultivo: agar marino, R2A y agar TCBS. Algunas de las bacterias fueron caracterizadas usando reacción en cadena de la polimerasa con análisis del polimorfismo de los fragmentos de restricción (PCR-RFLP) y secuenciación del gen ribosomal 16S. La respuesta inmune de larvas con el síndrome de "gaping jaws" fue medida por PCR en tiempo real. Los resultados evidenciaron diferencias significativas en la flora entre larvas normales y "gaping jaws". Las larvas c...

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Maternal transfer and transcriptional onset of immune genes during ontogenesis in Atlantic cod

Cited by 85 publications

References 53 publications

Evolutional Conservation of Molecular Structure and Antiviral Function of a Viral RNA Receptor, LGP2, in Japanese Flounder, Paralichthys olivaceus

Evolutional Conservation of Molecular Structure and Antiviral Function of a Viral RNA Receptor, LGP2, in Japanese Flounder, Paralichthys olivaceus

Effects of dietary β-glucan on maternal immunity and fry quality of rainbow trout (Oncorhynchus mykiss)

Characterization of culturable bacterial flora in yolk sac larvae of Atlantic halibut (Hippoglossus hippoglossus L.) with

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